Stenotrophomonas maltophilia: a new potential biocontrol agent of Ralstonia solanacearum, causal agent of potato brown rot

Abstract

Stenotrophomonas maltophilia was isolated from the rhizosphere of eggplant in the Nile Delta of Egypt, and its antagonistic potential against Ralstonia solanacearum race 3 biovar 2, the causal agent of potato brown rot, was in vitro evaluated on KB agar medium and in vivo on potato plants. In vitro, four isolates of S. maltophilia (PD3531, PD3532, PD3533, and PD3534) appeared antagonistic. The isolate (PD3533) was screened as the most promising antagonist for the in vivo tests. In the greenhouse, the antagonist was applied directly to soil or by bacterization of potato eyepieces. Stenotrophomonas maltophilia significantly suppressed potato brown rot in Egyptian clay soil but not in Dutch clay soil. Survival of a rifampicin and chloramphenicol-resistant S. maltophilia strain PD4560 was investigated in two pairs of clay soils, conventionally and organically managed, from Egypt and the Netherlands. The survival of S. maltophilia was significantly less in Dutch than in Egyptian soils, while the converse occurred for R. solanacearum. These results are in agreement with those obtained in the in vivo biocontrol tests. In conclusion, S. maltophilia may be useful for control of brown rot in the area where it was originally isolated, the Nile Delta in Egypt.

Pseudomonas corrugata and Pseudomonas marginalis associated with the collapse of tomato plants in rockwool slab hydroponic culture

“…P. synxantha is another fluorescent rhizosphere bacterium which has been recently placed in the P. flurescent group (Anzai et al 2000). Stenotrophomonas malthophilia, previously classified as Pseudomonas maltophilia, is ubiquitous in water, soil and rhizosphere of several cultured plants: cabbage, rape, mustard, maize, beet (Debette & Blondeau 1980) and potato (Messiha et al 2007). S. maltophilia is regarded as the emerging pathogen in human medicine.…”

“…It is used as a biological control agent against pre-and post-harvest plant pathogens (Catara 2007). Similarly, S. maltophilia may be useful for the control of ralstonia solanacearum, causal agent of potato brown rot (Messiha et al 2007).…”

Hygienic characteristics of radishes grown in soil contaminated with Stenotrophomonas maltophilia

“…Furthermore, the differences can also depend on the strainspecific characteristics. A continuous decrease of S. 7 CFU gdw −1 in different soil types with different management regimes at 28 ℃ was reported by Messiha et al [43] who studied the antagonistic effects of S. maltophilia against Ralstonia solanacearum. Those authors stated that the reasons for the differences in survival of S. maltophilia in the various soils were not clear, but supposed a direct effect of nitrate and ammonium contents and pH.…”

Controle biológico da murcha do tomateiro causada por Ralstonia solanacearum e Fusarium oxysporum f. sp. lycopersici por rizobactérias

“…A detecção de antibiose "in vitro" é um método que tem sido usado para seleção inicial de rizobactérias como possíveis agentes de biocontrole contra R. solanacearum (Moura & Romeiro, 1999;Lemessa & Zeller, 2007;Messiha et al, 2007.;Xue et al, 2009). No presente estudo, todas as rizobactérias produziram zona de inibição "in vitro" de R. solanacearum, portanto estes biocontroladores podem estar atuando por antibiose.…”