Stemphones, Novel Potentiators of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Aspergillus sp. FKI-2136

Koyama, Nobuhiro; Nagahiro, Tomoko; Yamaguchi, Yuichi; Masuma, Rokuro; Tomoda, Hiroshi; Ōmura, Satoshi

doi:10.1038/ja.2005.95

Cited by 36 publications

(36 citation statements)

References 20 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…15 Our research group has discovered microbial potentiators, such as stemphones from Aspergillus sp. 4,5 and cyslabdan from Streptomyces sp. 6,7 These potentiators have a polyphenol-or terpene-derived core structure.…”

Section: Discussionmentioning

confidence: 99%

“…7H 2 O, 0.020% KCl, 0.20% NaNO 3 , 0.020% yeast extract and 1.5% agar, adjusted to pH 6.0 before sterilization) was inoculated into a 50-ml tube containing 10 ml of the seed medium (2.0% glucose, 0.50% polypeptone, 0.050% MgSO 4 . 7H 2 O, 0.20% yeast extract, 0.10% KH 2 PO 4 and 0.10% agar, adjusted to pH 6.0 before sterilization).…”

Section: Fermentationmentioning

confidence: 99%

“…During our continuous screening program for microbial potentiators of imipenem activity against MRSA, [4][5][6][7] the culture broth of a fungal strain FKI-3765-2 was found to show potentiating activity. Activity-guided purification led to the discovery of two new compounds, namely designated xanthoradones A and B (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Xanthoradones, new potentiators of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Penicillium radicum FKI-3765-2: I. Taxonomy, fermentation, isolation and biological properties

et al. 2009

Self Cite

View full text Add to dashboard Cite

The fungal strain FKI-3765-2, identified as Penicillium radicum, was found to produce potentiators of imipenem activity against methicillin-resistant Staphylococcus aureus (MRSA). Two new compounds, designated xanthoradones A and B, were isolated from the fermentation broth of the producing strain by solvent extraction, octadecyl silyl column chromatography and preparative HPLC. Xanthoradones A and B potentiated imipenem activity against MRSA by decreasing the MIC value of imipenem from 16 lg ml À1 to 0.060 and 0.030 lg ml À1 , respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Fermentationmentioning

confidence: 99%

See 1 more Smart Citation

Xanthoradones, new potentiators of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Penicillium radicum FKI-3765-2: I. Taxonomy, fermentation, isolation and biological properties

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…16 and Technology of Japan and by a grant from the Uehara Memorial Foundation of Japan (to HT). We thank Dr H Hanaki for providing us with the MRSA K24 clinical strain.…”

Section: Assay Of Antimicrobial Activitymentioning

confidence: 99%

Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae. I: Fermentation, isolation and biological properties

et al. 2010

Self Cite

View full text Add to dashboard Cite

The in vivo-mimic assay system using silkworm larvae was used as a screening tool to discover antibiotics against methicillinresistant Staphylococcus aureus (MRSA). Microbial culture broths were screened in this in vivo-mimic assay system and a culture broth of Streptomyces sp. K04-0144 was selected. New antibiotics, designated nosokomycins A-D, were isolated from the culture broth by HP-20 and ODS column chromatography and HPLC. Nosokomycins inhibited the growth of MRSA with MIC values of 0.125 lg ml À1 using the liquid microdilution method. Furthermore, MRSA-infected silkworms survived when nosokomycin A or B was injected at a dose of 50 lg per larva.

show abstract

“…FKI-2136 [5]. During our continuous screening program, an actinomycete strain K04-0144 was found to produce a strong potentiator of imipenem activity against MRSA.…”

Section: Introductionmentioning

confidence: 99%

Cyslabdan, a New Potentiator of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Streptomyces sp. K04-0144

et al. 2008

Self Cite

View full text Add to dashboard Cite

Cyslabdan, a new potentiator of imipenem activity against methicillin-resistant Staphylococcus aureus, was isolated from the culture broth of Streptomyces sp. K04-0144 by Diaion HP-20 and ODS column chromatographies and preparative HPLC. The structure of cyslabdan was elucidated by spectroscopic analyses including NMR. The compound has a labdane-type diterpene skeleton connecting with an N-acetylcysteine via thioether linkage.Keywords cyslabdan, imipenem potentiator, methicillinresistant Staphylococcus aureus, MRSA, structural elucidation IntroductionMethicillin-resistant Staphylococcus aureus (MRSA) is known as a major nosocomial pathogen which has also developed resistance to many other antibiotics [1]. Moreover, MRSA getting resistant to the last-resort antibiotic, vancomycin, has been reported [2,3]. These facts suggest that MRSA would acquire more resistance to vancomycin in the near future. It is therefore increasingly important and necessary to find new antimicrobial agents and to devise new measures that are effective against MRSA infection.Based on the new concept of "anti-infective drugs" developed by Ō mura [4], a screening system was established to search for microbial potentiators of imipenem activity against MRSA, and new stemphones were recently discovered from the culture broth of Aspergillus sp. FKI-2136 [5]. During our continuous screening program, an actinomycete strain K04-0144 was found to produce a strong potentiator of imipenem activity against MRSA. Activity-guided purification lead to the discovery of a novel compound designated cyslabdan (Fig. 1). From the structural elucidation, the fundamental

show abstract

Stemphones, Novel Potentiators of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Aspergillus sp. FKI-2136

Cited by 36 publications

References 20 publications

Xanthoradones, new potentiators of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Penicillium radicum FKI-3765-2: I. Taxonomy, fermentation, isolation and biological properties

Xanthoradones, new potentiators of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Penicillium radicum FKI-3765-2: I. Taxonomy, fermentation, isolation and biological properties

Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae. I: Fermentation, isolation and biological properties

Cyslabdan, a New Potentiator of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Streptomyces sp. K04-0144

Contact Info

Product

Resources

About