StemCellDB: The Human Pluripotent Stem Cell Database at the National Institutes of Health

Mallon, Barbara S.; Chenoweth, Josh G.; Johnson, Kory R.; Hamilton, Rebecca S.; Tesar, Paul J.; Yavatkar, Amarendra S; Tyson, Leonard J.; Park, Kye-Yoon; Chen, Kevin G.; Fann, Yang C.; McKay, Ronald D.G.

doi:10.1016/j.scr.2012.09.002

Cited by 106 publications

(120 citation statements)

References 16 publications

Supporting

Mentioning

117

Contrasting

Order By: Relevance

“…The growth rates and doubling times for all cell lines are summarized in Table 1. The obtained doubling times are in agreement with previous measurements 26,27 , and vary considerably between hESC lines; H7 and H1 showed the fastest growth, while WA24 was the slowest.…”

Section: Resultssupporting

confidence: 91%

Effect of ionizing radiation on the proliferation of human embryonic stem cells

et al. 2017

View full text Add to dashboard Cite

We studied the effect of ionizing radiation (IR) on continuous growth of seven hESC lines. Cells were exposed to 0, 0.2, or 1 Gy of X-rays, and the growth rates of cell populations were assessed by measuring areas of the same individual colonies versus time. The population doubling times (DT) of sham-irradiated cells varied from 18.9 to 28.7 hours for different cell lines. All cell lines showed similar reaction to IR, i.e. cell populations dropped within 24-48 hours post IR; after that they recovered and grew with the same rate as the sham-irradiated cells. The relative cell survival (RCS), i.e. the ratio of normalized cell population in the irradiated samples to that of the sham-irradiated ones varied from 0.6 to 0.8 after 0.2 Gy, and from 0.1 to 0.2 after 1 Gy IR for different cell lines. We found that the RCS values of hESC lines correlated directly with their DT, i.e. the faster cells grow the more radiosensitive they are. We also found that DT and RCS values of individual colonies varied significantly within all hESC lines. We believe that the method developed herein can be useful for assessing other cytotoxic insults on cultures of hESC.Human embryonic stem cells (hESC) are unique models for studying genotoxic stresses including those produced by ionizing radiation (IR) because of their virtually unlimited proliferation potential 1,2 . In addition, their ability to differentiate into various tissues allows for studying the effects of IR on the developmental processes 3 . Studies of the effects of IR on hESC are also important because of the prospects of using these cells in regenerative medicine, which would require their transplantation or the transplantation of their differentiated products, and the imaging of the transplants with various techniques that may, like positron emission tomography (PET), utilize ionizing radiation 4 . During the past decade considerable knowledge was gained regarding the effects of IR on hESC 5 . It was shown that hESC are very sensitive to IR; the exposure to a relatively low dose of 1 Gy results in death of almost 30% of the cells 6 . The immediate effect of IR exposure of these cells is apoptosis; however, the surviving cells retain their pluripotency markers and ability to form all three embryonic germ layers as was evidenced by teratoma formation assay [6][7][8] . At the same time hESC have enhanced capacities to repair DNA damage as compared to differentiated cells 9 . It was found that these cells lack the G1/S checkpoint and stop their cell cycle progression after IR exposure by G2/M arrest instead [10][11][12] . Repair of DNA double strand breaks in hESC was shown to rely largely on homologous recombination [13][14][15] . A distinct feature of hESC is that in culture they propagate in tightly associated colonies 16 . When dissociated to a single cell suspension and plated back to a culture dish the majority of the cells die. Although treatments with certain factors can increase the plating efficiency of hESC 17,18 , proliferation in a colony is a normal physiological...

show abstract

Section: Resultssupporting

confidence: 91%

Effect of ionizing radiation on the proliferation of human embryonic stem cells

et al. 2017

View full text Add to dashboard Cite

show abstract

“…coriell.org) from patients with Parkinson's disease (AG20443, 71-year-old male; AG08395, 85-year-old female) and expanded as described previously [29]. Human BMSCs were harvested from surgical waste or bone marrow aspirates under institutionally approved protocols (NIH exemption #3113; NIH protocol #94-D-0183, respectively).…”

Section: Human Skin Fibroblast and Bone Marrow Stromal Cell Culturesmentioning

confidence: 99%

“…The two SF lines used in this study were reprogrammed to generate NIHi2 and NIHi7 using individual retroviral vectors (kindly provided by Shinya Yamanaka, Kyoto, Japan) for hOCT4, hSOX2, hKLF4, and hMYC [29] (supplemental online data). BMSCs were reprogrammed using the StemCCA lentiviral reprogramming kit (SCR-531; Millipore, Billerica, MA, http://www.millipore.com) according to the manufacturer's instructions (supplemental online data), and three human iPSC lines (SCUi1, SCUi8, and SCUi9) are reported here and were characterized for their pluripotential nature as described below.…”

Section: Human Ipsc Derivationmentioning

confidence: 99%

Directed Differentiation of Human Induced Pluripotent Stem Cells Toward Bone and Cartilage: In Vitro Versus In Vivo Assays

Phillips

Kuznetsov

Cherman

et al. 2014

Stem Cells Translational Medicine

Self Cite

View full text Add to dashboard Cite

The ability to differentiate induced pluripotent stem cells (iPSCs) into committed skeletal progenitors could allow for an unlimited autologous supply of such cells for therapeutic uses; therefore, we attempted to create novel bone-forming cells from human iPSCs using lines from two distinct tissue sources and methods of differentiation that we previously devised for osteogenic differentiation of human embryonic stem cells, and as suggested by other publications. The resulting cells were assayed using in vitro methods, and the results were compared with those obtained from in vivo transplantation assays. Our results show that true bone was formed in vivo by derivatives of several iPSC lines, but that the successful cell lines and differentiation methodologies were not predicted by the results of the in vitro assays. In addition, bone was formed equally well from iPSCs originating from skin or bone marrow stromal cells (also known as bone marrow-derived mesenchymal stem cells), suggesting that the iPSCs did not retain a "memory" of their previous life. Furthermore, one of the iPSC-derived cell lines formed verifiable cartilage in vivo, which likewise was not predicted by in vitro assays. STEM CELLS

show abstract

“…Moreover, the dose of reprogramming factors appears to affect CNVs [52], indicating a connection between epigenetics and genomic stability. Data on the differential epigenetic regulation of hESCs and iPSCs are somewhat controversial; the most recent comparison did not find any differences between isogenic hESCs and iPSCs [53,54], whereas others reported abnormalities in iPSC epigenomes [13,14,17,55,56]. Differences could also arise from iPSC epigenetic memory [48,57].…”

Section: Discussionmentioning

confidence: 99%

Mutation Frequency Dynamics inHPRTLocus in Culture-Adapted Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Correspond to Their Differentiated Counterparts

Krutá

Šeneklová

Raška

et al. 2014

Stem Cells and Development

View full text Add to dashboard Cite

The genomic destabilization associated with the adaptation of human embryonic stem cells (hESCs) to culture conditions or the reprogramming of induced pluripotent stem cells (iPSCs) increases the risk of tumorigenesis upon the clinical use of these cells and decreases their value as a model for cell biology studies. Base excision repair (BER), a major genomic integrity maintenance mechanism, has been shown to fail during hESC adaptation. Here, we show that the increase in the mutation frequency (MF) caused by the inhibition of BER was similar to that caused by the hESC adaptation process. The increase in MF reflected the failure of DNA maintenance mechanisms and the subsequent increase in MF rather than being due solely to the accumulation of mutants over a prolonged period, as was previously suggested. The increase in the ionizing-radiation-induced MF in adapted hESCs exceeded the induced MF in nonadapted hESCs and differentiated cells. Unlike hESCs, the overall DNA maintenance in iPSCs, which was reflected by the MF, was similar to that in differentiated cells regardless of the time spent in culture and despite the upregulation of several genes responsible for genome maintenance during the reprogramming process. Taken together, our results suggest that the changes in BER activity during the long-term cultivation of hESCs increase the mutagenic burden, whereas neither reprogramming nor long-term propagation in culture changes the MF in iPSCs.

show abstract

StemCellDB: The Human Pluripotent Stem Cell Database at the National Institutes of Health

Cited by 106 publications

References 16 publications

Effect of ionizing radiation on the proliferation of human embryonic stem cells

Effect of ionizing radiation on the proliferation of human embryonic stem cells

Directed Differentiation of Human Induced Pluripotent Stem Cells Toward Bone and Cartilage: In Vitro Versus In Vivo Assays

Mutation Frequency Dynamics inHPRTLocus in Culture-Adapted Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Correspond to Their Differentiated Counterparts

Contact Info

Product

Resources

About