Stem–loop binding protein expressed in growing oocytes is required for accumulation of mRNAs encoding histones H3 and H4 and for early embryonic development in the mouse

Arnold, Daniel; Françon, Patricia; Zhang, James; Martin, Kyle J.; Clarke, Hugh J.

doi:10.1016/j.ydbio.2007.10.032

Cited by 38 publications

(44 citation statements)

References 64 publications

(84 reference statements)

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“…34,82 A disruption of SLBP, a central regulator of histone mRNA metabolism, is deleterious for embryonic development of several organisms, resulting in chromosome condensation defect in Drosophila and C. elegans 83,84 and DNA replication failure in mouse oocytes. 85 Human somatic cells with depleted SLBP are viable, although their growth rate is significantly impaired due to a delayed progression through S phase as a result of inefficient DNA synthesis, [52][53][54] thus producing a similar phenotype as ABCE1 silencing in our study. It is hence tempting to speculate that the involvement in histone synthesis may be one of the vital functions of ABCE1.…”

Section: Discussionsupporting

confidence: 61%

ABCE1 is essential for S phase progression in human cells

et al. 2016

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ABCE1 is a highly conserved protein universally present in eukaryotes and archaea, which is crucial for the viability of different organisms. First identified as RNase L inhibitor, ABCE1 is currently recognized as an essential translation factor involved in several stages of eukaryotic translation and ribosome biogenesis. The nature of vital functions of ABCE1, however, remains unexplained. Here, we study the role of ABCE1 in human cell proliferation and its possible connection to translation. We show that ABCE1 depletion by siRNA results in a decreased rate of cell growth due to accumulation of cells in S phase, which is accompanied by inefficient DNA synthesis and reduced histone mRNA and protein levels. We infer that in addition to the role in general translation, ABCE1 is involved in histone biosynthesis and DNA replication and therefore is essential for normal S phase progression. In addition, we analyze whether ABCE1 is implicated in transcript-specific translation via its association with the eIF3 complex subunits known to control the synthesis of cell proliferation-related proteins. The expression levels of a few such targets regulated by eIF3A, however, were not consistently affected by ABCE1 depletion.

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Section: Discussionsupporting

confidence: 61%

ABCE1 is essential for S phase progression in human cells

et al. 2016

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“…Immunofluorescence analysis was performed as previously described (Arnold et al 2008). Briefly, after washing in PBS, samples were exposed to blocking buffer [3% (w/v) bovine serum albumin (BSA; Sigma) and 0.1% (v/v) Tween-20 (Sigma) in PBS] for 20 minutes at room temperature.…”

Section: Immunocytochemical Analysis Of Histone H3r26 Di-methylationmentioning

confidence: 99%

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

et al. 2015

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In vitro production (IVP) of bovine embryos is not only of great economic importance to the cattle industry, but is also an important model for studying embryo development. The aim of this study was to evaluate the histone modification, H3R26me2 during pre-implantation development of IVP bovine embryos cultured with or without serum supplementation and how these in vitro treatments compared to in vivo embryos at the morula stage. After in vitro maturation and fertilization, bovine embryos were cultured with either 0 or 2.5% fetal bovine serum (FBS). Development was evaluated and embryos were collected and fixed at different stages during development (2-, 4-, 8-, 16-cell, morula and blastocyst). Fixed embryos were then used for immunofluorescence utilizing an antibody for H3R26me2. Images of stained embryos were analyzed as a percentage of total DNA. Embryos cultured with 2.5% FBS developed to blastocysts at a greater rate than 0%FBS groups (34.85±5.43% vs. 23.38±2.93%; P<0.05). Levels of H3R26me2 changed for both groups over development. In the 0%FBS group, the greatest amount of H3R26me2 staining was at the 4-cell (P<0.05), 16-cell (P<0.05) and morula (P<0.05) stages. In the 2.5%FBS group, only 4-cell stage embryos were significantly higher than all other stages (P<0.01). Morula stage in vivo embryos had similar levels as the 0%FBS group, and both were significantly higher than the 2.5%FBS group. These results suggest that the histone modification H3R26me2 is regulated during development of pre-implantation bovine embryos, and that culture conditions greatly alter this regulation.

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“…Primer sequences were obtained as follows: mt-ND1 (Genbank NC_005089.1), Dr L C Smith (Université de Montréal); Polg, Polg2, Tfam, and Nrf1 ; rabbit globin cDNA (Adjaye et al 2007); and Actb (Arnold et al 2008). All were obtained from Sigma.…”

Section: Quantification Of Mtdnamentioning

confidence: 99%

Regulation of mitochondrial DNA accumulation during oocyte growth and meiotic maturation in the mouse

Mahrous¹,

Yang²,

Clarke³

2012

Reproduction

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Oocytes accumulate an enormous quantity of mitochondrial (mt) DNA, and an insufficient amount of mtDNA may underlie some cases of poor oocyte quality leading to infertility. Little is known, however, about the mechanisms that govern the timing and regulation of mtDNA accumulation during oogenesis. We report, through analysis of the mtDNA content of individual oocytes of the mouse, that mtDNA accumulates steadily during oocyte growth to reach a value of w175 000 copies per cell. MtDNA content ceases to increase once oocytes reach full size and remains unchanged during meiotic maturation. To test whether mtDNA accumulation depends on oocyte growth, we inhibited growth in vitro in two ways -by exposing complexes comprising partially grown oocytes enclosed by granulosa cells to a chemical inhibitor of the phosphatidylinositol-3-kinase signaling pathway and by removing the surrounding granulosa cells from partially grown oocytes. Under both conditions, the oocytes fail to grow, but mtDNA accumulation is unaffected, indicating that the two processes can be mechanistically uncoupled. Quantitative analysis of the mRNAs encoding proteins required for mtDNA replication revealed that Polg (Polga) (polymerase-g, a-subunit), Polg2 (Polgb), and Tfam (transcription factor A, mitochondrial) increase during oocyte growth but then decrease after fully grown oocytes become transcriptionally silent as indicated by the non-surrounded nucleolusto-surrounded nucleolus transition. Thus, there is a correlation between the decline in the quantity of mRNAs encoding mtDNA replication factors in fully grown oocytes and the arrest of mtDNA accumulation in these cells, suggesting that the two events may be causally linked.

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Stem–loop binding protein expressed in growing oocytes is required for accumulation of mRNAs encoding histones H3 and H4 and for early embryonic development in the mouse

Cited by 38 publications

References 64 publications

ABCE1 is essential for S phase progression in human cells

ABCE1 is essential for S phase progression in human cells

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

Regulation of mitochondrial DNA accumulation during oocyte growth and meiotic maturation in the mouse

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