Stem Cells Protect the Bronchial Stump in Rat, Increasing Sox6, Col2a1, and Agc1 Expression

Llontop, Pedro; Santana‐Rodríguez, Norberto; Clavo, Bernardino; Quintana, Ardiel; Fiuza, María D.; Camacho, Rafael; Rodriguez, Alfredo; Santana, C.F.S.D.; Ruiz-Caballero, José A.

doi:10.1007/s00408-014-9569-6

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…In another study, stem cell therapy decreased the necrosis and ulcerations of the bronchial stump in a rat model after pneumonectomy using adipose-derived stem cells (ASCs). A phenomenon was found where ASCs significantly increased the expression of COL2A1 after 10 days but decreased after 20 days [52]. A previous study showed that hMSCs produced more ECM on biomaterials' unique surfaces or environments [53].…”

Section: Gene Expression Of Hbmscs On the Hybrid Nanostructuresmentioning

confidence: 85%

“…hBMSCs could be stimulated into pre-chondrocytelike cells on the GlyCVD-BCC surface, but these cells are far from mature [42,43]. COL2 is important in many biological processes such as ECM synthesis, cellular responses to hormones, and mechanical stimulus [49][50][51][52]. In cellular responses to hormones, a previous study revealed reduced COL2 expression in the hypothyroid rat ovary, with a concomitant increase in COL2 degradation [51].…”

Section: Gene Expression Of Hbmscs On the Hybrid Nanostructuresmentioning

confidence: 99%

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Hybrid Surface Nanostructures Using Chemical Vapor Deposition and Colloidal Self-Assembled Patterns for Human Mesenchymal Stem Cell Culture—A Preliminary Study

et al. 2022

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Surface coatings are critical in biomaterials and biomedical devices. Chemical vapor deposition (CVD) is a well-known technology for the generation of thin films on a surface. However, the granular structures produced using CVD are rare. Recently, we used PPX-C, an excellent insulating material, for granular structure coating using CVD. Colloidal self-assembly is also a well-established method to generate granular structures named colloidal self-assembled patterns (cSAPs). In this study, we combined these two technologies to generate hierarchical granular structures and tested the biophysical effect of these hybrid surfaces on human bone marrow mesenchymal stem cells (hBMSCs). Two CVD-derived granular structures were made using water or glycerin droplets (i.e., CVD or GlyCVD surfaces). Water drops generate porous particles, while glycerin drops generate core–shell particles on the surface. These particles were dispersed randomly on the surface with sizes ranging from 1 to 20 μm. These CVD surfaces were hydrophobic (WCA ~ 80–110 degrees). On the other hand, a binary colloidal crystal (BCC), one type of cSAPs, composed of 5 μm Si and 400 nm carboxylated polystyrene (PSC) particles, had a close-packed structure and a hydrophilic surface (WCA ~ 45 degrees). The hybrid surfaces (i.e., CVD-BCC and GlyCVD-BCC) were smooth (Ra ~ 1.1–1.5 μm) and hydrophilic (WCA ~ 50 degrees), indicating a large surface coverage of BCC dominating the surface property. The hybrid surfaces were expected to be slightly negatively charged due to naturally charged CVD particles and negatively charged BCC particles. Cell adhesion was reduced on the hybrid surfaces, leading to an aggregated cell morphology, without reducing cell activity, compared to the flat control after 5 days. qPCR analysis showed that gene expression of type II collagen (COL2) was highly expressed on the GlyCVD-BCC without chemical induction after 3 and 14 days compared to the flat control. This proof-of-concept study demonstrates the potential of combining two technologies to make hybrid structures that can modulate stem cell attachment and differentiation.

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Section: Gene Expression Of Hbmscs On the Hybrid Nanostructuresmentioning

confidence: 85%

Section: Gene Expression Of Hbmscs On the Hybrid Nanostructuresmentioning

confidence: 99%

Hybrid Surface Nanostructures Using Chemical Vapor Deposition and Colloidal Self-Assembled Patterns for Human Mesenchymal Stem Cell Culture—A Preliminary Study

et al. 2022

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“…In contrast, cell therapy can be considered a new simple and minimally invasive method. The local administration of bone marrow stromal cells or adipose-derived stem cells to the bronchial stump increased fibrous connective tissues and the incidence of ulcers and necrosis was reduced in rat model, 11,12 and these cells have also been studied in large animal BPF models and clinically applied in humans. 25,26 Compared with these cells, fibroblasts can be obtained via minimally invasive procedures.…”

Section: Discussionmentioning

confidence: 99%

“…9,10 In some experiments, the local administration of bone marrow stromal cells or adipose-derived stem cells was effective in healing bronchial stumps in rat model. 11,12 A previous study showed that mixed sheets comprising fibroblasts and peripheral blood mononuclear cells secrete a large volume of vascular endothelial growth factor (VEGF) in vitro, and promoted angiogenesis and wound healing in a mouse skin ulcer model and rabbit lower limb ischemia model. 13,14 Furthermore, we developed a method of producing multilayered fibroblast sheets to increase the number of cells and improve ease of handling, and it proved to be effective in a mouse skin ulcer model.…”

Section: Perspective Statementmentioning

confidence: 99%

Autologous Multilayered Fibroblast Sheets Can Reinforce Bronchial Stump in a Rat Model

Yoshimine

Ueno

Murakami

et al. 2022

Seminars in Thoracic and Cardiovascular Surgery

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Adipose tissue-derived stem cells as a therapeutic strategy for enterocutaneous fistula: an experimental model study

Pagotto,

Camargo,

Cáceres

et al. 2023

Acta Cir. Bras.

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Purpose: Enterocutaneous fistula (ECF) is a condition in which there is an abnormal connection between the intestinal tract and the skin. It can lead to high morbidity and mortality rates despite the availability of therapeutic options. Stem cells have emerged as a potential strategy to treat ECF. This study aimed to evaluate the effect of adipose tissue-derived stem cells (ASC) on ECF in an experimental model. Methods: ECF was induced in 21 Wistar rats, and after one month, they were divided into three groups: control group (C), culture medium without ASC group (CM), and allogeneic ASC group (ASC). After 30 days, the animals underwent macroscopic analysis of ECF diameter and histopathological analysis of inflammatory cells, tissue fibrosis, and vascular density. Results: The study found a 55% decrease in the ECF diameter in the ASC group (4.5 ± 1.4 mm) compared to the control group (10.0 ± 2.1 mm, p = 0.001) and a 59.1% decrease in the CM group (11.0 ± 4.3 mm, p = 0.003). The fibrosis score in the ASC group was 20.9% lower than the control group (p = 0.03). There were no significant differences in inflammation scores among the three groups. Conclusions: This study suggests that ASC treatment can reduce ECF diameter, and reduction in tissue fibrosis may be a related mechanism. Further studies are needed to understand the underlying mechanisms fully.

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Stem Cells Protect the Bronchial Stump in Rat, Increasing Sox6, Col2a1, and Agc1 Expression

Abstract: Our findings indicate that local ASCs protected the bronchial stump after pneumonectomy and induced local changes in gene expression related to their protective action. These results could lead to a potential new therapeutic modality for the prevention of BPF.

Cited by 4 publications

References 19 publications

Hybrid Surface Nanostructures Using Chemical Vapor Deposition and Colloidal Self-Assembled Patterns for Human Mesenchymal Stem Cell Culture—A Preliminary Study

Hybrid Surface Nanostructures Using Chemical Vapor Deposition and Colloidal Self-Assembled Patterns for Human Mesenchymal Stem Cell Culture—A Preliminary Study

Autologous Multilayered Fibroblast Sheets Can Reinforce Bronchial Stump in a Rat Model

Adipose tissue-derived stem cells as a therapeutic strategy for enterocutaneous fistula: an experimental model study

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