Stem Cells in the Skin

Effective Labeling of Primary Somatic Stem Cells with BaTiO3 Nanocrystals for Second Harmonic Generation Imaging

“…selectable markers such as fluorescent and luminescent proteins have been widely used to track transplanted stem cells over long periods of time; [1][2][3][4] however, these markers have multiple limitations for intravital imaging, such as limited depth penetration in tissue, pronounced photo bleaching over time, and the presence of autofluorescence in surrounding tissues. [5] In addition, introducing exogenous genes may influence the function and behavior of stem cells.…”

“…[5] In addition, introducing exogenous genes may influence the function and behavior of stem cells. [3] To circumvent these limitations, a variety of inorganic nanoparticles have been proposed as alternative imaging agents for cell tracking. [1][2][3] Despite the wide variety of imaging modalities uti lized, tracking of stem cells in vivo at a high signaltonoise ratio (SNR) typically requires excess nanoparticle loading.…”

“…[3] To circumvent these limitations, a variety of inorganic nanoparticles have been proposed as alternative imaging agents for cell tracking. [1][2][3] Despite the wide variety of imaging modalities uti lized, tracking of stem cells in vivo at a high signaltonoise ratio (SNR) typically requires excess nanoparticle loading. These detection and tracking efforts can influence the stem cell biology or differ entiation potential of labeled cells, eventu ally compromising the outcome of stem cell therapies.…”