Stem cells in the arrangement of bone marrow repopulation and regenerative medicine

Balint, Bela; Stamatović, Dragana; Todorović, Milena; Jevtic, Miodrag; Ostojić, Gordana; Pavlović, Mirjana; Lojpur, Zvezdana; Jocić, Miodrag

doi:10.2298/vsp0707481b

Cited by 21 publications

(31 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This age limitation is regrettable since SC-transplant candidates in multiple myeloma are frequently older than 60 or even 65 years 1 . The earliest SC collections from PB were accomplished in "steady state hematopoiesis" -by numerous procedures since late 1970s, and following cryopreservation was needed 1,2,16 . The cell harvesting is the same for allogeneic donors as for autologous patients.…”

Section: Sem -Standard Error Of the Meanmentioning

confidence: 99%

See 1 more Smart Citation

Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Balint¹,

Stanojević

Todorović

et al. 2017

VOJNOSANIT PREGL

Self Cite

View full text Add to dashboard Cite

Background/Aim. Stem cells (SCs) guarantee complete/long-term bone marrow (BM) repopulation after SCtransplants. The aim of the study was to evaluate absolute count of total SCs (determined by ISHAGE-sequential-gating protocol -SC ish ) and relative frequency of immature CD34 + /CD90 + (CD90 + SC ish ) subset in peripheral blood (PB) as predictive factors of mobilization and apheresis product (AP) quality. Methods. Mobilization included chemotherapy and granulocyte-growth-factor (G-CSF). Harvesting was performed by Spectra-Optia-IDL-system. The SCs ish were determined as a constitutional part of CD34 + cells in the "stemcell-region" using FC-500 flow-cytometer. In this study, the original ISHAGE-sequential-gating protocol was modified by introduction of anti-CD90-PE monoclonal-antibody into the analysis of CD90 expression on SC ish (CD90 + SC ish ). The results were presented as a percentage of SC ish per nucleatedcell count, absolute SC ish count in μL of the PB or the AP, percentage of the CD90 + SC ish expressed to SC ish and absolute CD90 + SC ish count in μL of the PB or the AP. Results. The absolute count of total SC ish and CD90 + SC ish was significantly higher (p = 0.0007 and p = 0.0266, respectively) in the AP than in the PB samples. The CD90 + SC ish /total SC ish indexes from PB were higher than indexes from the AP (p = 0.039). Key words: stem cells; hematopoietic stem cell transplantation; bone marrow; flow cytometry; multiple myeloma; antineoplastic combined chemotherapy protocols.Apstrakt Uvod/Cilj. Matične ćelije (MĆ) obezbeđuju kompletnu/dugotrajnu repopulaciju kostne srži (KS) posle trans-plantacije. Cilj ove studije bila je procena apsolutnog broja ukupnih MĆ (utvrđena protokolom "ISHAGE-sequentialgating" -Stem-Cell ish [SC ish ]) i relativne učestalosti primitivnih podipova CD34 + /CD90 + (CD90 + SC ish ) u perifernoj krvi (PK) Ključne reči: ćelije, matične; transplantacija hematopoeznih matičnih ćelija; kostna srž; citometrija, protočna; multipli mijelom; lečenje kombinovanjem antineoplastika, protokoli.

show abstract

Section: Sem -Standard Error Of the Meanmentioning

confidence: 99%

“…High-dose chemotherapy followed by allogeneic or autologous SC-transplants is considered as standard treatment for some malignant and few immune-mediated diseases (e.g. multiple sclerosis) [1][2][3] . The use of SCs for organ repair (damaged myocardium, liver, pancreas, etc) opens new perspectives in regenerative medicine 1,3 .…”

Section: Introductionmentioning

confidence: 99%

Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Balint¹,

Stanojević

Todorović

et al. 2017

VOJNOSANIT PREGL

Self Cite

View full text Add to dashboard Cite

show abstract

“…Generally, SCs can be divided into embryonic and adult cell compartment, but several adult SCs have a similar high-level ("unlimited") biological potential to embryonic cells [2,4]. Thus, some adult SCs are able to develop into a variety of somatic cells by using the features of "plasticity" or "trans-differentiation" [5][6][7][8][9]. Although the term "plasticity" has become very popular, some studies have suggested that BM might contain different types of SCs that can produce nonhematopoietic (somatic) cells.…”

Section: Sažetakmentioning

confidence: 99%

“…Although the term "plasticity" has become very popular, some studies have suggested that BM might contain different types of SCs that can produce nonhematopoietic (somatic) cells. For example, mesenchymal SCs (MSCs)/pericytes (that is contractile cells that wrap around endothelial cells) from BM can switch to osteocytes, chondrocytes, adipocytes and skeletal muscle cells [2,4,8]. Consequently, these cells are applicable in the field of regenerative medicine, i.e.…”

Section: Sažetakmentioning

confidence: 99%

Stem cell transplant: From cell harvesting to cryopreservation

Balint¹,

Todorović

Urošević

et al. 2017

Med pregl

Self Cite

View full text Add to dashboard Cite

Stem cells could be defined as cells capable for self-renewal with high proliferative capacity and extensive potential to differentiate into blood cells or some somatic cell types - ?plasticity? due to ?trans-differentiation? - such as osteocytes, chondrocytes, hepatocytes, myocytes, cardiomyocytes and even endothelial cells. Recent increasing clinical use of various cell-mediated therapeutic approaches has resulted in amplified needs for both stem cells and operating procedures to get a minimized cell damages during collection, purification and cryopreservation. The aim of cell harvesting procedures is to obtain the best stem cells yield, high purity and good viability/clonogenicity. The goal of optimized cryoinvestigation protocols is to minimize cell injuries during the freeze/thaw process (cryoinjury). Despite the fact that different stem cells collection protocols and cell freezing practice are already in routine use, a lot of questions related to the optimal blood stem cells harvesting, purification and cryopreservation are still unresolved.

show abstract

“…Regenerative medicine is a recently developed multidisciplinary discipline aimed to replace or restore normal function of cells, tissues, and organs that are damaged by disease [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Unlike conventional surgical therapies, which, although including some element of reconstruction, remain predominantly ablative for most diseases, regenerative medicine incorporates the fields of tissue engineering, cell biology, nuclear transfer and materials science to allow a truly regenerative approach, by reconstructing, repairing, or replacing missing or damaged tissue to a state as close as possible to its native architecture and function.…”

Section: Introductionmentioning

confidence: 99%

Amniotic Fluid Stem Cells: a Promising Therapeutic Resource for Cell-Based Regenerative Therapy

Antonucci

Pantalone

Tetè

et al. 2012

CPD

View full text Add to dashboard Cite

Stem cells have been proposed as a powerful tool in the treatment of several human diseases, both for their ability to represent a source of new cells to replace those lost due to tissue injuries or degenerative diseases, and for the ability of produce trophic molecules able to minimize damage and promote recovery in the injured tissue. Different cell types, such as embryonic, fetal or adult stem cells, human fetal tissues and genetically engineered cell lines, have been tested for their ability to replace damaged cells and to restore the tissue function after transplantation. Amniotic fluid -derived Stem cells (AFS) are considered a novel resource for cell transplantation therapy, due to their high renewal capacity, the "in vitro" expression of embryonic cell lineage markers, and the ability to differentiate in tissues derived from all the three embryonic layers. Moreover, AFS do not produce teratomas when transplanted into animals and are characterized by a low antigenicity, which could represent an advantage for cell transplantation or cell replacement therapy. The present review focuses on the biological features of AFS, and on their potential use in the treatment of pathological conditions such as ischemic brain injury and bone damages.

show abstract

Stem cells in the arrangement of bone marrow repopulation and regenerative medicine

Cited by 21 publications

References 21 publications

Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Stem cell transplant: From cell harvesting to cryopreservation

Amniotic Fluid Stem Cells: a Promising Therapeutic Resource for Cell-Based Regenerative Therapy

Contact Info

Product

Resources

About