Stem Cells in Skeletal Tissue Engineering: Technologies and Models

Langhans, Mark T.; Yu, Shuting; Tuan, Rocky S.

doi:10.2174/1574888x10666151001115248

Cited by 14 publications

(10 citation statements)

References 280 publications

(285 reference statements)

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“…Stem cells and tissue-derived stromal cells stimulate the repair of degenerated and injured tissues, motivating a growing number of cell-based therapies in the musculoskeletal field [ 1 , 2 ]. Mesenchymal stem cells (MSCs) showed a good self-renew ability and were capable of differentiating into the progeny of several lineages, including osteoblasts, chondrocytes, adipocytes, fibroblasts, tenocytes, and myoblasts [ 3 – 7 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells

Zhang

Chen

et al. 2020

Stem Cells International

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Current cell-based therapies on musculoskeletal tissue regeneration were mostly determined in rodent models. However, a direct translation of those promising cell-based therapies to humans exists a significant hurdle. For solving this problem, canine has been developed as a new large animal model to bridge the gap from rodents to humans. In this study, we reported the isolation and characterization of urine-derived stem cells (USCs) from mature healthy beagle dogs. The isolated cells showed fibroblast-like morphology and had good clonogenicity and proliferation. Meanwhile, these cells positively expressed multiple markers of MSCs (CD29, CD44, CD90, and CD73), but negatively expressed for hematopoietic antigens (CD11b, CD34, and CD45). Additionally, after induction culturing, the isolated cells can be differentiated into osteogenic, adipogenic, chondrogenic, and tenogenic lineages. The successful isolation and verification of USCs from canine were useful for studying cell-based therapies and developing new treatments for musculoskeletal injuries using the preclinical canine model.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells

Zhang

Chen

et al. 2020

Stem Cells International

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“…In order to develop a clinical practice using MSCs, expansion of MSC populations in vitro is critical to achieving a therapeutically relevant number of active cells [4]. In cases where the body cannot heal itself via natural stem cell recruiting pathways the cause is often a defect in stem cell induction and signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

Lawrence

Cottrill

Valluri

et al. 2019

IJMS

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Lack of standardization of clinically compliant culture protocols of mesenchymal stem cells for re-implantation in humans have hindered clinical progress in the field of tissue regeneration to repair maxillofacial and orthopedic defects. The goal of this study was to establish a clinically relevant osteogenic protocol for collection and expansion of autologous stem cells to be used at Marshall University for re-implantation and repair of maxillofacial and orthopedic conditions. Human bone marrow (hBM) samples were collected from patients undergoing intramedullary nail fixation for closed femoral fractures. hBM mesenchymal cells were expanded by growing them first in Petri dishes for two weeks, followed by a week of culture using Perfecta 3D Hanging Drop Plates®. Various scaffold materials were tested and analyzed for cellular integration, vitality, and differentiation capacity of harvested hBM-MSCs including: 60/40 blend of hydroxyapatite biomatrix; Acellular bone composite discs; Allowash®, cancellous bone cubes; PLGA (poly lactic-co-glycolic acid); and Woven chitin derived fiber. We found that the 3D spheroid culture allowed production of hBM mesenchymal cells that retained osteoblast differentiation capacity over a monolayer culture of hBM-MSCs without the need to use chemical or hormonal modulation. We also observed that hydroxyapatite and Allowash cancellous bone scaffolds allowed better cell integration and viability properties as compared to other materials tested in this study. In conclusion, the multimodal culture methodology we developed creates actively differentiating stem-cell spheroids that can then be readily utilized in clinical practices to improve the regeneration of tissues of the head and the body.

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“…The graft with the biomaterial used in this study promoted osteoconduction which, in turn, refers to the ability of a surface to be favorable to adequate bone growth, usually through pores, channels or tubes. The biomaterial allows the recruitment and stimulation of pluripotent and undifferentiated cells into the form of bone-forming cells, such as pre-osteoblasts, osteoblasts, and ultimately osteocytes 29 .…”

Section: ■ Discussionmentioning

confidence: 99%