Stem cells in pathobiology and regenerative medicine

Alison,

doi:10.1002/path.2497

Cited by 10 publications

(7 citation statements)

References 25 publications

(23 reference statements)

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“…The research on the potential therapeutic use of LPCs has accelerated significantly in recent years giving rise to a vast amount of data on the power of regeneration of the liver driven by hepatocytes and LPCs [4,17,36,86] (Fig. 1).…”

Section: Hepatocyte Mediated Liver Regenerationmentioning

confidence: 98%

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The quest for liver progenitor cells: A practical point of view

Dollé

Best

Mei

et al. 2010

Journal of Hepatology

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Many chronic liver diseases can lead to hepatic dysfunction with organ failure. At present, orthotopic liver transplantation represents the benchmark therapy of terminal liver disease. However this practice is limited by shortage of donor grafts, the need for lifelong immunosuppression and very demanding state-of-the-art surgery. For this reason, new therapies have been developed to restore liver function, primarily in the form of hepatocyte transplantation and artificial liver support devices. While already offered in very specialized centers, both of these modalities still remain experimental. Recently, liver progenitor cells have shown great promise for cell therapy, and consequently they have attracted a lot of attention as an alternative or supportive tool for liver transplantation. These liver progenitor cells are quiescent in the healthy liver and become activated in certain liver diseases in which the regenerative capacity of mature hepatocytes and/or cholangiocytes is impaired. Although reports describing liver progenitor cells are numerous, they have not led to a consensus on the identity of the liver progenitor cell. In this review, we will discuss some of the characteristics of these cells and the different ways that have been used to obtain these from rodents. We will also highlight the challenges that researchers are facing in their quest to identify and use liver progenitor cells.

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Section: Hepatocyte Mediated Liver Regenerationmentioning

confidence: 98%

“…This review does not address the controversial issue of the hepatocytic potential of bone marrow-derived stem cells, nor discusses the challenges encountered in cell culture; these topics have been covered extensively in other recent reviews [4,61,104,148].…”

Section: Introductionmentioning

confidence: 99%

The quest for liver progenitor cells: A practical point of view

Dollé

Best

Mei

et al. 2010

Journal of Hepatology

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“…Stem cells are defined by their distinctive capability to self‐renew and produce differentiated progeny during development and throughout the entire life of an organism. Owing to their unique abilities, stem cells have rapidly been identified as an unprecedented source of clinically relevant differentiated cells for application in tissue engineering and regenerative medicine [1] and as in vitro (disease) models for drug discovery and trials [2]. Despite extensive research and our ever‐growing knowledge in stem cell biology, the field is still confronted by a lack of reproducible and reliable methods to control stem cell behavior.…”

Section: Introductionmentioning

confidence: 99%

Concise Review: Tailoring Bioengineered Scaffolds for Stem Cell Applications in Tissue Engineering and Regenerative Medicine

Cosson

Otte

Hezaveh

et al. 2015

Stem Cells Translational Medicine

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The potential for the clinical application of stem cells in tissue regeneration is clearly significant. However, this potential has remained largely unrealized owing to the persistent challenges in reproducibly, with tight quality criteria, and expanding and controlling the fate of stem cells in vitro and in vivo. Tissue engineering approaches that rely on reformatting traditional Food and Drug Administrationapproved biomedical polymers from fixation devices to porous scaffolds have been shown to lack the complexity required for in vitro stem cell culture models or translation to in vivo applications with high efficacy. This realization has spurred the development of advanced mimetic biomaterials and scaffolds to increasingly enhance our ability to control the cellular microenvironment and, consequently, stem cell fate. New insights into the biology of stem cells are expected to eventuate from these advances in material science, in particular, from synthetic hydrogels that display physicochemical properties reminiscent of the natural cell microenvironment and that can be engineered to display or encode essential biological cues. Merging these advanced biomaterials with high-throughput methods to systematically, and in an unbiased manner, probe the role of scaffold biophysical and biochemical elements on stem cell fate will permit the identification of novel key stem cell behavioral effectors, allow improved in vitro replication of requisite in vivo niche functions, and, ultimately, have a profound impact on our understanding of stem cell biology and unlock their clinical potential in tissue engineering and regenerative medicine. STEM CELLS TRANSLATIONAL MEDICINE 2015;4:156-164

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“…Stem and progenitor cells have been used in experimental and clinical trials to treat a wide range of diseases 23, 24. However, the underlying mechanisms remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Secreted proteome of the murine multipotent hematopoietic progenitor cell line DKmix

Luecke

Templin

Muetzelburg

et al. 2010

Rapid Comm Mass Spectrometry

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Administration of the multipotent hematopoietic progenitor cell (HPC) line DKmix improved cardiac function after myocardial infarction and accelerated dermal wound healing due to paracrine mechanisms. The aim of this study was to analyse the secreted proteins of DKmix cells in order to identify the responsible paracrine factors and assess their relevance to the wide spectrum of therapeutic effects. A mass spectrometry (MS)-based approach was used to identify secreted proteins of DKmix cells. Serum free culture supernatants of DKmix-conditioned medium were collected and the proteins present were separated, digested by trypsin and the resulting peptides were then analyzed by matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) MS. Overall 95 different proteins were identified. Among them, secretory proteins galectin-3 and gelsolin were identified. These proteins are known to stimulate cell migration and influence wound healing and cardiac remodelling. The remaining proteins originate from intracellular compartments like cytoplasm (69%), nucleus (12%), mitochondria (4%), and cytoplasmic membrane (3%) indicating permeable or leaky DKmix cells in the conditioned medium. Additionally, a sandwich immunoassay was used to detect and quantify cytokines and chemokines. Interleukin-6 (IL-6), interleukin-13 (IL-13), monocyte-chemoattractant protein-1 (MCP-1), monocyte-chemoattractant protein-3 (MCP-3), monocyte-chemoattractant protein-1alpha (MIP-1alpha) and monocyte-chemoattractant protein-1beta (MIP-1beta) were detected in low concentrations. This study identified a subset of proteins present in the DKmix-conditioned medium that act as paracrine modulators of tissue repair. Moreover, it suggests that DKmix-derived conditioned medium might have therapeutic potency by promoting tissue regeneration.

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Stem cells in pathobiology and regenerative medicine

Cited by 10 publications

References 25 publications

The quest for liver progenitor cells: A practical point of view

The quest for liver progenitor cells: A practical point of view

Concise Review: Tailoring Bioengineered Scaffolds for Stem Cell Applications in Tissue Engineering and Regenerative Medicine

Secreted proteome of the murine multipotent hematopoietic progenitor cell line DKmix

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