Stem Cells in Lung Repair and Regeneration

Lane, S.; Rippon, Helen J.; Bishop, Anne E.

doi:10.2217/17460751.2.4.407

Cited by 25 publications

(11 citation statements)

References 66 publications

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“…These cells may be dormant within tissue but would proliferate under circumstances of injury and repair [3,4,5]. The dynamics of tissue stem cells or progenitor cells varies from tissue to tissue; for example, in bone marrow, liver, lung, and gut, stem cells regularly proliferate to supplement cells during normal turnover or injury [6,7,8,9], while in the pancreas, the heart, or the nervous system they proliferate to replace damaged cells following injury [10,11,12,13,14]. …”

Section: Introductionmentioning

confidence: 99%

Introduction to Stem Cells and Regenerative Medicine

Kolios

Moodley²

2012

Respiration

369

259

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Stem cells are a population of undifferentiated cells characterized by the ability to extensively proliferate (self-renewal), usually arise from a single cell (clonal), and differentiate into different types of cells and tissue (potent). There are several sources of stem cells with varying potencies. Pluripotent cells are embryonic stem cells derived from the inner cell mass of the embryo and induced pluripotent cells are formed following reprogramming of somatic cells. Pluripotent cells can differentiate into tissue from all 3 germ layers (endoderm, mesoderm, and ectoderm). Multipotent stem cells may differentiate into tissue derived from a single germ layer such as mesenchymal stem cells which form adipose tissue, bone, and cartilage. Tissue-resident stem cells are oligopotent since they can form terminally differentiated cells of a specific tissue. Stem cells can be used in cellular therapy to replace damaged cells or to regenerate organs. In addition, stem cells have expanded our understanding of development as well as the pathogenesis of disease. Disease-specific cell lines can also be propagated and used in drug development. Despite the significant advances in stem cell biology, issues such as ethical controversies with embryonic stem cells, tumor formation, and rejection limit their utility. However, many of these limitations are being bypassed and this could lead to major advances in the management of disease. This review is an introduction to the world of stem cells and discusses their definition, origin, and classification, as well as applications of these cells in regenerative medicine.

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Section: Introductionmentioning

confidence: 99%

Introduction to Stem Cells and Regenerative Medicine

Kolios

Moodley²

2012

Respiration

369

259

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“…Based on prior studies [20], we used mice foetal pulmonary cells (FPCs) as seeding cells to reconstruct lung tissues in vitro. [34][35][36]. The AE2 cell has parallel board of layered structures, known as the osmiophilic lamellar body (the main component of the small body of phospholipids).…”

Section: Discussionmentioning

confidence: 99%

The reconstruction of lung alveolus-like structure in collagen-matrigel/microcapsules scaffolds in vitro

Zhang

Lin

Zhang

et al. 2011

Journal of Cellular and Molecular Medicine

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This study attempted to use collagen–Matrigel as extracellular matrix (ECM) to supply cells with three-dimensional (3D) culture condition and employ alginate-poly-l-lysine-alginate (APA) microcapsules to control the formation of alveolus-like structure in vitro. We tested mice foetal pulmonary cells (FPCs) by immunohistochemistry after 2D culture. The alveolus-like structure was reconstructed by seeding FPCs in collagen–Matrigel mixed with APA microcapsules 1.5 ml. A self-made mould was used to keep the structure from contraction. Meanwhile, it provided static stretch to the structure. After 7, 14 and 21 days of culture, the alveolus-like structure was analysed histologically and immunohistochemically, or by scanning transmission electron microscopy (TEM). We also observed these structures under inverted phase contrast microscope. The expression of pro-surfactant protein C (SpC) was detected by reverse transcription-polymerase chain reaction (RT-PCR). We obtained fibroblasts, epithelial cells and alveolar type II (AE2) cells in FPCs. In the reconstructed structure, seeding cells surrounding the APA microcapsules constructed alveolus-like structures, the size of them ranges from 200 to 300 μm. In each reconstructed lung tissue sheet, microcapsules had integrity. Pan-cytokeratin, vimentin and SpC positive cells were observed in 7- and 14-day cultured structures. TEM showed lamellar bodies of AE2 cells in the reconstructed tissues whereas RT-PCR expressed SpC gene. Primary mice FPCs could form alveolus-like structures in collagen–Matrigel/APA microcapsules engineered scaffolds, which could maintain a differentiated state of AE2 cells.

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“…Stem cells and progenitor cells are of obvious interest on the basis of their ability to expand in culture and differentiate into multiple cell lineages, including ATII, bronchial epithelial, or pulmonary endothelial cells (18,36,52,97,160). However, the use of stem cells requires extensive knowledge about the spatiotemporal mechanisms underlying lung development, which is relatively well understood in mouse models and to a lesser extent in humans (90,223), raising the question about the approach to be taken in the use of human-derived stem cells to induce lung cell lineages.…”

Section: Recellularization In Lung Bioengineeringmentioning

confidence: 99%