Stem Cells from Healthy and Tendinopathic Human Tendons: Morphology, Collagen and Cytokines Expression and Their Response to T3 Thyroid Hormone

Ciardulli, Maria Camilla; Scala, Pasqualina; Giudice, Valentina; Selleri, Carmine; Oliva, Francesco; Maffulli, Nicola; Porta, Giovanna Della

doi:10.3390/cells11162545

Cited by 7 publications

(6 citation statements)

References 66 publications

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“…Increasing TNF-α and CXCL-12 also prove NF-κB pathway involvement either the classical pathway and non-classical pathway, respectively. In accordance with our results, previous study performed in University of Salemo, using tendinopathy induced human tendon tissue (18), also showed increasing inflammatory marker such as TNF-α, IL-1β, and IL-6. Interestingly, TGF-β (tumor growth factor β) also increased in this model, indicating tissue recovery.…”

Section: Discussionsupporting

confidence: 93%

“…There are several changes occurred such as increasing inflammatory immune responses, collagen fiber degradation, and cellular transformation (10,17). Dysregulation of matrix metalloproteinase 7 and matrix metalloproteinase 3 also has been proposed as pathogenesis of Achilles tendinopathy (18). Some study also mentioned transformation of tenocyte in Achilles tendinopathy where the tenocyte tends be to more ovoid, produces more proteoglycans, and seems like chondrocyte (18).…”

Section: Discussionmentioning

confidence: 99%

“…Dysregulation of matrix metalloproteinase 7 and matrix metalloproteinase 3 also has been proposed as pathogenesis of Achilles tendinopathy (18). Some study also mentioned transformation of tenocyte in Achilles tendinopathy where the tenocyte tends be to more ovoid, produces more proteoglycans, and seems like chondrocyte (18). Therefore, more advanced and deeper study must perform especially in vivo.…”

Section: Discussionmentioning

confidence: 99%

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Epidemiology of Shoulder Arthroplasty in a Southern-Italy Region: A Three-Year Registry Study

Handoyo¹,

Hidayat²,

Sardjono³

et al. 2023

Muscle Ligaments and Tendons J

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Background. As a common ailment suffered by runners and athletes, Achilles tendinopathy pathogenesis is not well understood. To reveal its cellular and molecular changes, universal and standardized method to induce Achilles tendinopathy in animal model (in vivo) study should be established. There are 2 major guidelines to induce Achilles tendinopathy: run the rats on treadmill without inclination and run the rats on treadmill with 10° inclination. Objective. To compare available Achilles tendinopathy induction procedure hence determining the most effective procedure. Methods. Laboratory experimental animal study (in vivo) with post-test only control group approach. 2 procedures to induce Achilles tendinopathy on Wistar rats were performed: running at 15 m/min for 180 minutes, and running at 25 m/min for 15 minutes with 10° inclination for 60 minutes. Histological changes of Achilles tendon were converted into Bonar score while several inflammatory markers such as extracellular high mobility group box-1 (HMGB-1), tumor necrosis factor-alpha (TNF-α), C-X-C motif chemokine ligand-12 (CXCL-12), and macrophage-2 (M2) were measured. Results. Highest Bonar score was obtained from rats that run at 25 m/min for 15 minutes with 10° inclination for 60 minutes. Highest level of extracellular HMGB-1, TNF-α, and CXCL-12 was also obtained from similar group. This group also showed widest M2 coverage area. Conclusions. Running the rats at 25 m/min for 15 minutes with 10° inclination for 60 minutes is more effective than running the rats at 15 m/min for 180 minutes without inclination to induce Achilles tendinopathy.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Epidemiology of Shoulder Arthroplasty in a Southern-Italy Region: A Three-Year Registry Study

Handoyo¹,

Hidayat²,

Sardjono³

et al. 2023

Muscle Ligaments and Tendons J

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“…Statistical analysis was performed using ordinary one-way analysis of variance (ANOVA) test for independent groups, after checking data normality and homogeneity. p values less than 0.05 were accepted as significant [ 53 , 54 , 55 , 56 ]. All statistical analysis was conducted using GraphPad Prism software (6.0 for Windows, LLC, San Diego, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Activity and Selectivity of Novel Chemical Metallic Complexes with Potential Anticancer Effects on Melanoma Cells

et al. 2023

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Human malignant melanoma cells from lymph node metastatic site (MeWo) were selected for testing several synthesized and purified silver(I) and gold(I) complexes stabilized by unsymmetrically substituted N-heterocyclic carbene (NHC) ligands, called L20 (N-methyl, N′-[2-hydroxy ethylphenyl]imidazol-2-ylide) and M1 (4,5-dichloro, N-methyl, N′-[2-hydroxy ethylphenyl]imidazol-2-ylide), having halogenide (Cl− or I−) or aminoacyl (Gly=N-(tert-Butoxycarbonyl)glycinate or Phe=(S)-N-(tert-Butoxycarbonyl)phenylalaninate) counterion. For AgL20, AuL20, AgM1 and AuM1, the Half-Maximal Inhibitory Concentration (IC50) values were measured, and all complexes seemed to reduce cell viability more effectively than Cisplatin, selected as control. The complex named AuM1 was the most active just after 8 h of treatment at 5 μM, identified as effective growth inhibition concentration. AuM1 also showed a linear dose and time-dependent effect. Moreover, AuM1 and AgM1 modified the phosphorylation levels of proteins associated with DNA lesions (H2AX) and cell cycle progression (ERK). Further screening of complex aminoacyl derivatives indicated that the most powerful were those indicated with the acronyms: GlyAg, PheAg, AgL20Gly, AgM1Gly, AuM1Gly, AgL20Phe, AgM1Phe, AuM1Phe. Indeed, the presence of Boc-Glycine (Gly) and Boc-L-Phenylalanine (Phe) showed an improved efficacy of Ag main complexes, as well as that of AuM1 derivatives. Selectivity was further checked on a non-cancerous cell line, a spontaneously transformed aneuploid immortal keratinocyte from adult human skin (HaCaT). In such a case, AuM1 and PheAg complexes resulted as the most selective allowing HaCaT viability at 70 and 40%, respectively, after 48 h of treatment at 5 μM. The same complexes tested on 3D MeWo static culture induced partial spheroid disaggregation after 24 h of culture, with almost half of the cells dead.

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“… 93 – 96 They are released by the tendon stroma or immunoregulatory cells in response to mechanical stress or tissue injury, alter the cellular phenotype and induce changes in matrix production. 97 , 98 Adequate mechanical stimuli play an essential role in tendon homeostasis, regular function, tenocyte survival and tendon healing and mechanical factors influence tendon cytokine profile. 99 An increased amount of pro-inflammatory cytokines such as IL-1a, IL-1b, TNF-α and IFN-γ was demonstrated in inflamed native equine tendon.…”

Section: Tendon Mechanical Propertiesmentioning

confidence: 99%

Tendon tissue engineering: An overview of biologics to promote tendon healing and repair

Citro,

Clerici,

Boccaccini

et al. 2023

J Tissue Eng

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Tendons are dense connective tissues with a hierarchical polarized structure that respond to and adapt to the transmission of muscle contraction forces to the skeleton, enabling motion and maintaining posture. Tendon injuries, also known as tendinopathies, are becoming more common as populations age and participation in sports/leisure activities increases. The tendon has a poor ability to self-heal and regenerate given its intrinsic, constrained vascular supply and exposure to frequent, severe loading. There is a lack of understanding of the underlying pathophysiology, and it is not surprising that disorder-targeted medicines have only been partially effective at best. Recent tissue engineering approaches have emerged as a potential tool to drive tendon regeneration and healing. In this review, we investigated the physiochemical factors involved in tendon ontogeny and discussed their potential application in vitro to reproduce functional and self-renewing tendon tissue. We sought to understand whether stem cells are capable of forming tendons, how they can be directed towards the tenogenic lineage, and how their growth is regulated and monitored during the entire differentiation path. Finally, we showed recent developments in tendon tissue engineering, specifically the use of mesenchymal stem cells (MSCs), which can differentiate into tendon cells, as well as the potential role of extracellular vesicles (EVs) in tendon regeneration and their potential for use in accelerating the healing response after injury.

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Stem Cells from Healthy and Tendinopathic Human Tendons: Morphology, Collagen and Cytokines Expression and Their Response to T3 Thyroid Hormone

Cited by 7 publications

References 66 publications

Epidemiology of Shoulder Arthroplasty in a Southern-Italy Region: A Three-Year Registry Study

Epidemiology of Shoulder Arthroplasty in a Southern-Italy Region: A Three-Year Registry Study

Activity and Selectivity of Novel Chemical Metallic Complexes with Potential Anticancer Effects on Melanoma Cells

Tendon tissue engineering: An overview of biologics to promote tendon healing and repair

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