Stem cells from adipose tissue

Witkowska-Zimny, Małgorzata; Walenko, Katarzyna

doi:10.2478/s11658-011-0005-0

Cited by 83 publications

(78 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Adipose tissue stem cells can be easily harvested from adipose tissue and expanded, with capacity of differentiation on multiple lineages in vitro 21 . Many adipose tissue stem cells isolation methods have been developed since Rodbell 28 , who demonstrated that adipocytes can be isolated from the stromal vascular fraction.…”

Section: Discussionmentioning

confidence: 99%

Characterization of human adipose-derived stem cells

Gaiba¹,

França²,

França³

et al. 2012

Acta Cir. Bras.

View full text Add to dashboard Cite

PURPOSE:There is a growing scientific interest in the plasticity and therapeutic potential of adipose-derived stem cells (ASCs), which are multipotent and abundant in adipose tissue and can differentiate in vitro into multiple lineages, including adipocytes, chondrocytes, osteoblasts, neural cells, endothelial cells and cardiomyocytes. The aim of this study was to isolate, cultivate and identify ASCs. METHODS: Human adipose precursor cells were obtained from subcutaneous abdominal tissue. Recently dispersed cells were separated by density centrifugation gradient, cultured and then analyzed. RESULTS: Human ASCs were able to replicate in our culture conditions. The cells maintained their phenotypes throughout the studied period on different passages confirming they suitability for in vitro cultivation. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. Flow cytometry results showed that these cells expressed CD73, CD90 and CD105, (mesenchymal stem-cells markers), contrasting with the lack of expression of CD16, CD34 and CD45 (hematopoietic cells markers). RESUMO OBJETIVO:Há um interesse científico crescente na plasticidade e potencial terapêutico das células-tronco do tecido adiposo humano, células multipotentes e abundantes no tecido adiposo que podem se diferenciar in vitro em múltiplas linhagens celulares, incluindo adipócitos, condrócitos, osteoblastos, células neurais, endoteliais e cardiomiócitos. O objetivo deste estudo foi isolar, cultivar e identificar células-tronco do tecido adiposo humano. MÉTODOS: Células precursoras humanas do tecido adiposo foram obtidas de tecido abdominal subcutâneo. As células recém-dispersas foram separadas por gradiente de centrifugação por densidade, cultivadas e então analisadas. RESULTADOS: As células-tronco do tecido adiposo humano foram capazes de se replicar nas nossas condições de cultivo e mantiveram seu fenótipo em diferentes passagens durante o estudo, confirmando sua adequabilidade para cultivo in vitro. A diferenciação adipogênica, osteogênica e condrogênica também foi induzida, confirmando seu potencial de células-tronco mesenquimais in vitro. Os resultados de citometria de fluxo evidenciaram a expressão dos marcadores de células-tronco mesenquimais CD73, CD90 e CD105, contrastando com a falta de expressão dos marcadores de células hematopoiéticas CD16, CD34 e CD45. CONCLUSÃO: Foi possível isolar células-tronco do tecido adiposo humano por cultivo in vitro sem indução adipogênica, mantendo sua integridade funcional e altos níveis de proliferação. As células demonstraram potencial de diferenciação adipogênico, osteogênico e condrogênico in vitro. Descritores: Adipócitos. Células-Tronco Adultas. Células-Tronco Mesenquimais. Células-Tronco. Tecido Adiposo.Gaiba S et al.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of human adipose-derived stem cells

Gaiba¹,

França²,

França³

et al. 2012

Acta Cir. Bras.

View full text Add to dashboard Cite

show abstract

“…Adipose tissue is easily accessible via liposuction or abdominoplasty and has been shown to contain a higher number of stem cells/progenitors than bone marrow tissue [10]. The extracted cells also expand more rapidly than those from other tissues [11]. We and other groups have shown that rat ASC can be differentiated toward a Schwann cell-like phenotype which promotes neurite outgrowth [12][13][14][15][16] and myelination [16][17][18].…”

mentioning

confidence: 99%

Stimulating the Neurotrophic and Angiogenic Properties of Human Adipose-Derived Stem Cells Enhances Nerve Repair

Kingham

Kolar

Novikova

et al. 2014

Stem Cells and Development

184

147

View full text Add to dashboard Cite

show abstract

“…WAT specializes in energy storage and the production and decomposition of fats, thus acting as the body's metabolic reserve [5,8]. Adipose tissue is currently the focus of considerable research because it has been recognized that its stromal-vascular cell fraction (SVF) can be an abundant source of multipotent and pluripotent stem cells and adipose-derived stem cells, also called adipose tissue-derived stromal cells (ADSCs) [14,15]. WAT is a better material for the isolation of ADSCs for several reasons.…”

Section: Adipose Tissue As An Easily Accessible and Abundant Source Omentioning

confidence: 99%

Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications

Harasymiak-Krzyżanowska

Niedojadło

Karwat

et al. 2013

Cellular and Molecular Biology Letters

View full text Add to dashboard Cite

The stromal-vascular cell fraction (SVF) of adipose tissue can be an abundant source of both multipotent and pluripotent stem cells, known as adipose-derived stem cells or adipose tissue-derived stromal cells (ADSCs). The SVF also contains vascular cells, targeted progenitor cells, and preadipocytes. Stromal cells isolated from adipose tissue express common surface antigens, show the ability to adhere to plastic, and produce forms that resemble fibroblasts. They are characterized by a high proliferation potential and the ability to differentiate into cells of meso-, ecto- and endodermal origin. Although stem cells obtained from an adult organism have smaller capabilities for differentiation in comparison to embryonic and induced pluripotent stem cells (iPSs), the cost of obtaining them is significantly lower. The 40 years of research that mainly focused on the potential of bone marrow stem cells (BMSCs) revealed a number of negative factors: the painful sampling procedure, frequent complications, and small cell yield. The number of stem cells in adipose tissue is relatively large, and obtaining them is less invasive. Sampling through simple procedures such as liposuction performed under local anesthesia is less painful, ensuring patient comfort. The isolated cells are easily grown in culture, and they retain their properties over many passages. That is why adipose tissue has recently been treated as an attractive alternative source of stem cells. Essential aspects of ADSC biology and their use in regenerative medicine will be analyzed in this article.

show abstract

Stem cells from adipose tissue

Cited by 83 publications

References 106 publications

Characterization of human adipose-derived stem cells

Characterization of human adipose-derived stem cells

Stimulating the Neurotrophic and Angiogenic Properties of Human Adipose-Derived Stem Cells Enhances Nerve Repair

Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications

Contact Info

Product

Resources

About