Stem Cell Transplantation and Cell-Free Treatment for Periodontal Regeneration

Iwasaki, Kengo; Peng, Yihao; Kanda, Ryuhei; Umeda, Makoto; Ishikawa, Isao

doi:10.3390/ijms23031011

Cited by 23 publications

(17 citation statements)

References 84 publications

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“…DFSCs have showed considerable advantages in promoting periodontal tissue regeneration [ 16 , 34 , 58 ]. However, there are still many difficulties in the clinical application of stem cells, especially possible utility, safety, and ethical issues [ 59 ]. Molecular drugs may be relatively more convenient, safe, and stable [ 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Dental Follicle Stem Cells Promote Periodontal Regeneration through Periostin-Mediated Macrophage Infiltration and Reprogramming in an Inflammatory Microenvironment

Wei

Guo

Liu

et al. 2023

IJMS

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Dental follicle stem cells (DFSCs) have been verified to promote periodontal regeneration in an inflammatory microenvironment. When coping with inflammatory stimulation, DFSCs highly express periostin, a bioactive molecule closely related to periodontal homeostasis. It is worth exploring whether and how periostin plays a role in the promotion of periodontal regeneration by DFSCs. By tracking the fate of DFSCs, it was found that DFSCs significantly contributed to periodontal regeneration in rat periodontal defects while they had a low survival rate. They highly expressed periostin and improved the immune microenvironment in the defect area, especially via the recruitment and reprogramming of macrophages. Silencing periostin attenuated the effects of DFSCs in promoting periodontal regeneration and regulating macrophages. Recombinant human periostin (rhPeriostin) could not only directly promote macrophage reprogramming through the integrin αM/phosphorylated extracellular signal-regulated kinase (p-Erk)/Erk signaling pathway, but it also exhibited the potential to promote periodontal regeneration in rats when loaded in a collagen matrix. These results indicated that periostin is actively involved in the process by which DFSCs promote periodontal regeneration through the regulation of macrophages and is a promising molecular agent to promote periodontal regeneration. This study provides new insight into the mechanism by which DFSCs promote periodontal regeneration and suggests a new approach for periodontal regeneration therapy.

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Section: Discussionmentioning

confidence: 99%

Dental Follicle Stem Cells Promote Periodontal Regeneration through Periostin-Mediated Macrophage Infiltration and Reprogramming in an Inflammatory Microenvironment

Wei

Guo

Liu

et al. 2023

IJMS

View full text Add to dashboard Cite

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“…Various stem cell transplantation techniques have been used, such as direct injection of stem cells grown in culture medium at the site of the periodontal defect [36], using a sponge impregnated with stem cells, or injecting the culture medium with enriched stem cells [79]. The use of carriers to implant stem cells in the place of a periodontal defect reduces the viability and proliferation of these cells; therefore, the direct injection method is preferred [80].…”

Section: Induced Pluripotent Stem Cells (Ipscs)mentioning

confidence: 99%

The Role and Involvement of Stem Cells in Periodontology

et al. 2023

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Periodontitis is a widespread inflammatory condition, characterized by a progressive deterioration of the supporting structures of the teeth. Due to the complexity of periodontal tissue and the surrounding inflammatory microenvironment, the repair of lesions at this level represents a continuous challenge. The regeneration of periodontal tissues is considered a promising strategy. Stem cells have remarkable properties, such as immunomodulatory potential, proliferation, migration, and multilineage differentiation. Thus, they can be used to repair tissue damage and reduce inflammation, potentially leading to periodontal regeneration. Among the stem cells used for periodontal regeneration, we studied dental mesenchymal stem cells (DMSCs), non-dental stem cells, and induced pluripotent stem cells (IPSCs). Although these cells have well documented important physiological characteristics, their use in contemporary practice to repair the affected periodontium is still a challenge.

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“…Since then, numerous studies have shown that MSCs can be cultured, and MSCs can differentiate beyond mesenchymal lineages, such as bone, cartilage, and fat, to mesodermal tissues, including muscle, in vitro and ex vivo . Several preclinical studies and clinical trials have also shown the efficacy of MSC transplantation [23] , [24] , [25] . However, unlike hematopoietic stem cells, which are more strictly defined and can form the entire hematopoietic system by single cells, MSCs are heterogeneous, and single cells do not form entire tissues.…”

Section: Stem Cell Populations In Periodontal Tissuementioning

confidence: 99%