Stem Cell Therapy in Autoimmune Rheumatic Diseases: a Comprehensive Review

Liu, Bin; Shu, Shang-An; Kenny, Thomas P.; Chang, Christopher; Leung, Patrick S.C.

doi:10.1007/s12016-014-8445-8

Cited by 13 publications

(5 citation statements)

References 128 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TLR2 is activated upon recognition of PAMPs, which bind to the intracellular adaptor protein MyD88 to activate a number of signaling pathways that lead to the expression of pro-inflammatory cytokines (24). To investigate if OV could inhibit TLR2 signaling by interfering with TLR2-MyD88 interaction, a Co-IP assay was performed with lysates from MPC5 cells with or without OV treatment and LPS stimulation using an anti-MyD88 antibody.…”

Section: Resultsmentioning

confidence: 99%

Treatment with toll‑like receptor 2 inhibitor ortho‑vanillin alleviates lipopolysaccharide‑induced acute kidney injury in mice

et al. 2019

View full text Add to dashboard Cite

Reducing inflammation is a promising approach for the prevention and treatment of septic acute kidney injury (AKI), since AKI is characterized by excessive inflammation in the kidney. Previous studies have demonstrated that toll-like receptor 2 (TLR2) is overstimulated, which promotes inflammation by activating the NF-κB signaling pathway, in a lipopolysaccharide (LPS)-induced model of AKI mice. For the present study, it was hypothesized that TLR2 inhibition could reduce inflammation and consequently prevent septic AKI. Therefore, the potential renal protective effects of ortho-vanillin (OV), an inhibitor of TLR2, were investigated in the present study in vitro and in vivo. In vitro treatment with OV on LPS-stimulated mouse podocyte cell line MPC5 did not affect TLR2 expression but interrupted the interaction between TLR2 and its downstream adaptor MyD88, resulting in the reduction of inflammatory cytokines IL-6 and TNF-α expression. In vivo OV treatment in an LPS-challenged mouse model effectively alleviated LPS-induced kidney injury as indicated by histology analysis and the significantly reduced blood urea nitrogen and serum creatinine levels. Additionally, inflammatory cytokines TNF-α, IL-6 and IL-1β expression were also significantly reduced in mice with OV treatment. Signaling pathway analysis further demonstrated that OV treatment did not affect the expression of TLR2 and p65 but suppressed p65 phosphorylation. Taken together, data from the present study demonstrated that OV was effective in protecting renal function against LPS-induced AKI through the inhibition of TLR2/NF-κB signaling and subsequent inflammatory cytokine production. These findings indicated that OV or targeting TLR2 signaling in general, represents a novel therapeutic approach for use in the prevention and treatment of AKI.

show abstract

Section: Resultsmentioning

confidence: 99%

Treatment with toll‑like receptor 2 inhibitor ortho‑vanillin alleviates lipopolysaccharide‑induced acute kidney injury in mice

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Mesenchymal stem cells (MSCs) represent an optimal tool for tissue regeneration in a clinical setting due to their multipotency, being able to differentiate into various cells (bone, cartilage, fat, muscle, and tendon) under appropriate conditions. This particular property together with their low immunogenicity and the ability to secrete bioactive factors (cytokines, chemokines, and growth factors) in response to local microenvironmental cues, makes them an ideal candidate for the treatment of different pathologies (Dalal, Gandy, & Domen, ; Gnecchi, Danieli, & Cervio, ; Liu, Shu, Kenny, Chang, & Leung, ; Park, Kim, Lee, Park, & An, ).…”

Section: Introductionmentioning

confidence: 99%

Autocrine signals increase ovine mesenchymal stem cells migration through Aquaporin‐1 and CXCR4 overexpression

Pelagalli¹,

Nardelli

Lucarelli

et al. 2018

Journal Cellular Physiology

View full text Add to dashboard Cite

Sheep is a relevant large animal model that is frequently used to test innovative tissue engineering (TE) approaches especially for bone reconstruction. Mesenchymal stem cells (MSCs) are used in TE applications because they represent key component of adult tissue repair. Importantly, MSCs from different species show similar characteristics, which facilitated their application in translational studies using animal models. Nowadays, many researches are focusing on the use of ovine mesenchymal stem cells (oMSCs) in orthopedic preclinical settings for regenerative medicine purposes. Therefore, there is a need to amplify our knowledge on the mechanisms underlying the behaviour of these cells. Recently, several studies have shown that MSC function is largely dependent on factors that MSCs release in the environment, as well as, in conditioned medium (CM). It has been demonstrated that MSCs through autocrine and paracrine signals are able to stimulate proliferation, migration, and differentiation of different type of cells including themselves. In this study, we investigated the effects of the CM produced by oMSCs on oMSCs themselves and we explored the signal pathways involved. We observed that CM caused an enhancement of oMSC migration. Furthermore, we found that CM increased levels of two membrane proteins involved in cell migration, Aquaporin 1 (AQP1), and C-X-C chemokine receptor type 4 (CXCR4), and activated Akt and Erk intracellular signal pathways. In conclusion, taken together our results suggest the high potential of autologous CM as a promising tool to modulate behaviour of MSCs thus improving their use in therapeutically approaches.

show abstract

“…Supported by animal models of antigen‐induced autoimmunity, the primary goal of this therapeutic approach is to achieve drug‐free remission by elimination of the disease‐mediating immune cells and re‐establishment of immune regulation, thereby restoring self‐tolerance. Numerous phase I‐II studies have tested autologous HSCT in diseases, such as multiple sclerosis, systemic sclerosis, systemic lupus erythematosus, rheumatoid arthritis and juvenile idiopathic arthritis [reviewed in (Liu et al , ; Openshaw et al , )]. Most commonly, the graft is T‐cell depleted, because lympho‐depletion of disease‐causing cells is vital.…”

Section: Future Directionsmentioning

confidence: 99%

The evolution of T‐cell depletion in haploidentical stem‐cell transplantation

Or-Geva

Reisner

2015

Br J Haematol

View full text Add to dashboard Cite

T-cell depletion (TCD) can prevent the onset of graft-versus-host disease (GvHD) in animal models of bone marrow transplantation; this manipulation enabled the successful application in the 1980s of T-cell depleted bone marrow (BM) for the treatment of babies with severe combined immune deficiency (SCID). However, in leukaemia patients, implementation of T-cell depletion has been more difficult, especially due to high rate of graft-rejection, leukaemia relapse and delayed immune reconstitution. These hurdles were gradually overcome by modifying the cell composition of the graft, and by reducing the toxicities associated with conditioning protocols. Although no 'gold standard' TCD method exists, T-cell depletion in its modern forms could offer clinical benefit, even for patients with a matched sibling donor.

show abstract

Stem Cell Therapy in Autoimmune Rheumatic Diseases: a Comprehensive Review

Cited by 13 publications

References 128 publications

Treatment with toll‑like receptor 2 inhibitor ortho‑vanillin alleviates lipopolysaccharide‑induced acute kidney injury in mice

Treatment with toll‑like receptor 2 inhibitor ortho‑vanillin alleviates lipopolysaccharide‑induced acute kidney injury in mice

Autocrine signals increase ovine mesenchymal stem cells migration through Aquaporin‐1 and CXCR4 overexpression

The evolution of T‐cell depletion in haploidentical stem‐cell transplantation

Contact Info

Product

Resources

About