Stem cell therapy for central nerve system injuries: glial cells hold the key

Xiao, Li; Saiki, Chikako; Ide, Ryoji

doi:10.4103/1673-5374.137570

Cited by 34 publications

(19 citation statements)

References 126 publications

(134 reference statements)

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“…Additionally, the pore size of the electrospun PLLA MTAM was sufficiently small to prevent the infiltration of astrocytes (cultured on the outer surfaces of the electrospun PLLA MTAM) while allowing signaling molecules critical for cross-talk between astrocytes and nerve stem cells. Ultimately, this cross-talk will promote proliferation and growth of the cells as evident in work by other groups [Xiao et al, 2014].…”

Section: Discussionmentioning

confidence: 92%

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

Tseng¹,

Chew²,

Huang³

et al. 2017

Cells Tissues Organs

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Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. In this study, the interaction between rat fetal neural stem cells (NSC) and astrocytes was examined by comparing the outcome of a typical Transwell-based coculture system and that of an electrospun PLLA MTAM-based coculture system. Compared to tissue culture polystyrene (TCP) and Transwell coculture inserts, a superior cell viability of NSC was observed when cultured in lumens of electrospun PLLA MTAM (with supportive immunostaining images). Reverse transcription polymerase chain reaction revealed a strong interaction between astrocytes and NSC through a higher expression of doublecortin and a lower expression of nestin. These data demonstrate that MTAM is clearly a better coculture platform than the traditional Transwell system.

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Section: Discussionmentioning

confidence: 92%

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

Tseng¹,

Chew²,

Huang³

et al. 2017

Cells Tissues Organs

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“…The adult mammalian central nerve system (CNS) presents inherent difficulties for its effective regeneration following traumatic injuries or neurodegenerative diseases, such as spinal cord injury, stroke and Alzheimer’s disease [ 1 ]. The greatest problem in overcoming neuronal damage and achieving successful CNS recovery is the difficulty in regenerating functional neurons in the CNS [ 2 , 3 , 4 ]. Exogenous cellular replacement and endogenous cell stimulation were considered the fundamental approaches for neuroregeneration.…”

Section: Introductionmentioning

confidence: 99%

Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro

Xiao

Ide

Saiki

et al. 2017

IJMS

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The adult mammalian central nerve system has fundamental difficulties regarding effective neuroregeneration. The aim of this study is to investigate whether human dental pulp cells (DPCs) can promote neuroregeneration by (i) being differentiated toward neuronal cells and/or (ii) stimulating local neurogenesis in the adult hippocampus. Using immunostaining, we demonstrated that adult human dental pulp contains multipotent DPCs, including STRO-1, CD146 and P75-positive stem cells. DPC-formed spheroids were able to differentiate into neuronal, vascular, osteogenic and cartilaginous lineages under osteogenic induction. However, under neuronal inductive conditions, cells in the DPC-formed spheroids differentiated toward neuronal rather than other lineages. Electrophysiological study showed that these cells consistently exhibit the capacity to produce action potentials, suggesting that they have a functional feature in neuronal cells. We further co-cultivated DPCs with adult mouse hippocampal slices on matrigel in vitro. Immunostaining and presto blue assay showed that DPCs were able to stimulate the growth of neuronal cells (especially neurons) in both the CA1 zone and the edges of the hippocampal slices. Brain-derived neurotrophic factor (BDNF), was expressed in co-cultivated DPCs. In conclusion, our data demonstrated that DPCs are well-suited to differentiate into the neuronal lineage. They are able to stimulate neurogenesis in the adult mouse hippocampus through neurotrophic support in vitro.

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“…We performed bioinformatic analysis on the Hispanic-Latino (H) and Asian (A) replicate lines H3.1.1/H3.3.3 and A2.2.2/A2.1.1 as contractile/non-contractile pairs. Since cardiac differentiation is driven through regulation of TGF-β signaling mediated by NODAL, Activin and BMP ligands38394950 we evaluated genes in this pathway. In the A2.2.2/A2.1.1 contractile/non-contractile pair, NODAL (2.0X) and BMP10 (8X) were upregulated in ED-iPSCs for contractile cells.…”

Section: Discussionmentioning

confidence: 99%

Distinct and Shared Determinants of Cardiomyocyte Contractility in Multi-Lineage Competent Ethnically Diverse Human iPSCs

Tomov

Olmsted

Dogan

et al. 2016

Sci Rep

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The realization of personalized medicine through human induced pluripotent stem cell (iPSC) technology can be advanced by transcriptomics, epigenomics, and bioinformatics that inform on genetic pathways directing tissue development and function. When possible, population diversity should be included in new studies as resources become available. Previously we derived replicate iPSC lines of African American, Hispanic-Latino and Asian self-designated ethnically diverse (ED) origins with normal karyotype, verified teratoma formation, pluripotency biomarkers, and tri-lineage in vitro commitment. Here we perform bioinformatics of RNA-Seq and ChIP-seq pluripotency data sets for two replicate Asian and Hispanic-Latino ED-iPSC lines that reveal differences in generation of contractile cardiomyocytes but similar and robust differentiation to multiple neural, pancreatic, and smooth muscle cell types. We identify shared and distinct genes and contributing pathways in the replicate ED-iPSC lines to enhance our ability to understand how reprogramming to iPSC impacts genes and pathways contributing to cardiomyocyte contractility potential.

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Stem cell therapy for central nerve system injuries: glial cells hold the key

Cited by 34 publications

References 126 publications

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro

Distinct and Shared Determinants of Cardiomyocyte Contractility in Multi-Lineage Competent Ethnically Diverse Human iPSCs

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