Stem Cell Therapy and Exercise for Treatment of Intervertebral Disc Degeneration

Li, Bo; Yang, Yunmei; Wang, Lan; Liu, Guanghua

doi:10.1155/2021/7982333

Cited by 19 publications

(13 citation statements)

References 95 publications

(93 reference statements)

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“…Our increasing understanding of IDD pathology has led to more therapeutic approaches. Current treatment covers almost all scales of disc tissue from macro to micro levels, such as gene therapy, cell replenishment and surgical treatment ( Debono et al, 2018 ; Li et al, 2021 ; Roh et al, 2021 ). Limited by many factors such as ethics, biocompatibility and persistence, IDD therapy, which regulates the metabolism and repairment of cells and tissues, appears to be more popular.…”

Section: The Application Of Different Transmission Systemsmentioning

confidence: 99%

Application of single and cooperative different delivery systems for the treatment of intervertebral disc degeneration

Liu

2022

Front. Bioeng. Biotechnol.

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Intervertebral disc (IVD) degeneration (IDD) is the most universal pathogenesis of low back pain (LBP), a prevalent and costly medical problem across the world. Persistent low back pain can seriously affect a patient’s quality of life and even lead to disability. Furthermore, the corresponding medical expenses create a serious economic burden to both individuals and society. Intervertebral disc degeneration is commonly thought to be related to age, injury, obesity, genetic susceptibility, and other risk factors. Nonetheless, its specific pathological process has not been completely elucidated; the current mainstream view considers that this condition arises from the interaction of multiple mechanisms. With the development of medical concepts and technology, clinicians and scientists tend to intervene in the early or middle stages of intervertebral disc degeneration to avoid further aggravation. However, with the aid of modern delivery systems, it is now possible to intervene in the process of intervertebral disc at the cellular and molecular levels. This review aims to provide an overview of the main mechanisms associated with intervertebral disc degeneration and the delivery systems that can help us to improve the efficacy of intervertebral disc degeneration treatment.

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Section: The Application Of Different Transmission Systemsmentioning

confidence: 99%

Application of single and cooperative different delivery systems for the treatment of intervertebral disc degeneration

Liu

2022

Front. Bioeng. Biotechnol.

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“…Clinically, low back pain is one of the most medical problems in spine surgery affecting 70%–85% of the population worldwide ( 1 ). Many causes could lead to low back pain, while intervertebral disk degeneration (IVDD) is considered as the most common reason ( 2 ). Currently, the main clinical therapeutic approaches regarding IVDD include conservative treatment and surgery.…”

Section: Introductionmentioning

confidence: 99%

Identification of a potential novel biomarker in intervertebral disk degeneration by bioinformatics analysis and experimental validation

Yang

Yuan

2023

Front. Immunol.

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BackgroundIntervertebral disk degeneration (IVDD) is a major cause of low back pain and one of the most common health problems all over the world. However, the early diagnosis of IVDD is still restricted. The purpose of this study is to identify and validate the key characteristic gene of IVDD and analyze its correlation with immune cell infiltration.Methods3 IVDD-related gene expression profiles were downloaded from the Gene Expression Omnibus database to screen for differentially expressed genes (DEGs). Gene Ontology (GO) and gene set enrichment analysis (GSEA) were conducted to explore the biological functions. Two machine learning algorithms were used to identify characteristic genes, which were tested to further find the key characteristic gene. The receiver operating characteristic curve was performed to estimate the clinical diagnostic value of the key characteristic gene. The excised human intervertebral disks were obtained, and the normal nucleus pulposus (NP) and degenerative NP were carefully separated and cultured in vitro. The expression of the key characteristic gene was validated by real-time quantitative PCR (qRT-PCR). The related protein expression in NP cells was detected by Western blot. Finally, the correlation was investigated between the key characteristic gene and immune cell infiltration.ResultsA total of 5 DEGs, including 3 upregulated genes and 2 downregulated genes, were screened between IVDD and control samples. GO enrichment analysis showed that DEGs were enriched to 4 items in BP, 6 items in CC, and 13 items in MF. They mainly included the regulation of ion transmembrane transport, transporter complex, and channel activity. GSEA suggested that the cell cycle, DNA replication, graft versus host disease, and nucleotide excision repair were enriched in control samples, while complement and coagulation cascades, Fc γ R–mediated phagocytosis, neuroactive ligand–receptor interaction, the NOD-like receptor signaling pathway, gap junctions, etc., were enriched in IVDD samples. Furthermore, ZNF542P was identified and tested as key characteristic gene in IVDD samples through machine learning algorithms and showed a good diagnostic value. The results of qRT-PCR showed that compared with normal NP cells, the expression of ZNF542P gene was decreased in degenerated NP cells. The results of Western blot suggested that compared with normal NP cells, the expression of NLRP3 and pro Caspase-1 was increased in degenerated NP cells. Finally, we found that the expression of ZNF542P was positively related to the proportions of T cells gamma delta (γδT cells).ConclusionZNF542P is a potential biomarker in the early diagnosis of IVDD and may be associated with the NOD-like receptor signaling pathway and the infiltration of γδT cells.

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“…In terms of cells, physical exercise could effectively promote the cell proliferation of NP and AF, thereby promoting the repair of IVDs [15][16][17] . Currently, most studies explain the benefits of physical exercise on IVDD in terms of mechanical stimulation 17 , muscle strength 18,19 , and nutritional metabolism 20,21 . However, the molecular mechanism underlying the beneficial effect of physical exercise on IVDD has yet to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Exercise-induced FNDC5/irisin protects nucleus pulposus cells against senescence and apoptosis by activating autophagy

et al. 2022

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Intervertebral disc degeneration (IVDD) is a major cause of low back pain (LBP), and excessive senescence and apoptosis of nucleus pulposus (NP) cells are major pathological changes in IVDD. Physical exercise could effectively delay the process of intervertebral disc degeneration; however, its mechanism is still largely unknown. Irisin is an exercise-induced myokine released upon cleavage of the membrane-bound precursor protein fibronectin type III domain-containing protein 5 (FNDC5), and its levels increase after physical exercise. Here, we show that after physical exercise, FNDC5/irisin levels increase in the circulation and NP, senescence and apoptosis are reduced, autophagy is activated in NP tissue, and the progression of IVDD is delayed. Conversely, after knocking out FNDC5, the benefits of physical exercise are compromised. Moreover, the overexpression of FNDC5 in NP tissue effectively alleviated the degeneration of the intervertebral disc (IVD) in rats. By showing that FNDC5/irisin is an important mediator of the beneficial effects of physical exercise in the IVDD model, the study proposes FNDC5/irisin as a novel agent capable of activating autophagy and protecting NP from senescence and apoptosis.

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Stem Cell Therapy and Exercise for Treatment of Intervertebral Disc Degeneration

Cited by 19 publications

References 95 publications

Application of single and cooperative different delivery systems for the treatment of intervertebral disc degeneration

Application of single and cooperative different delivery systems for the treatment of intervertebral disc degeneration

Identification of a potential novel biomarker in intervertebral disk degeneration by bioinformatics analysis and experimental validation

Exercise-induced FNDC5/irisin protects nucleus pulposus cells against senescence and apoptosis by activating autophagy

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