Stem Cell Therapies for Male Infertility: Where Are We Now and Where Are We Going?

Valli, Hanna; Gassei, Kathrin; Orwig, Kyle E.

doi:10.1007/978-3-319-17849-3_3

Cited by 14 publications

(14 citation statements)

References 164 publications

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“…Studies showed that testicular sperm count, motility, and testicular weight were significantly decreased in diabetic rats (Valli et al, 2015). Diabetes induced testicular damage is associated with low seminiferous tubular diameter (STD) which may be due to a low level of gonadotropins and testosterone (Castro et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Allium cepa (Onion) Seeds (AC) Extract on Histopathology of Testis in STZ-Induced Male Rats

Fallah

Mahabadi

et al. 2017

Int. J. Morphol.

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FALLAH, V.; MAHABADI, J. A.; MAHABADI, M. Y.; KASHANI, H. H. & NIKZAD, N.Protective effect of Allium cepa (onion) seeds (AC) extract on histopathology of testis in stz-induced male rats. Int. J. Morphol., 35(4):1517Morphol., 35(4): -1524Morphol., 35(4): , 2017. SUMMARY:The aim of the present study was to evaluate the effect of the extract of Allium cepa (Onion) seeds (AC) on morphometric and histology of testis and biochemical parameters in STZ-induced male rats. Forty adult male Wistar rats (2 month old) were allocated into four groups of control, diabetic control, diabetic treated with 200 or 400 mg/kg/day of onion seed extract. Diabetes mellitus was induced using 60 mg/kg body weight of Streptozotocin as a single intraperitoneal injection. The extract was administered by stomach gavage for 28 days. The morphometric and histological structure of the testis, biochemical factors like glucose and testosterone levels were assessed. All analyses were done at the end of the four week study period. Data were compared by using Kruskal Wallis Test, Dunnett T3 and the degree of significance was set at P < 0.05 and P < 0.01. In diabetic+200 rats, the numbers of primary spermatocytes were significantly increased. In diabetic+400 rats, seminiferous tubular diameter was significantly increased and the level of testosterone hormone and testis weight was decreased significantly. In diabetic+200 and 400 rats, the numbers of spermatid, FBS and lumen diameter were significantly increased and the numbers of spermatozoa cells, body weight and volume density (VD) % lumen were decreased. Also, the numbers of spermatid in control diabetic rats was decreased. Our finding indicated that onion seed extract might be useful as a supplementary protective agent against adverse effects of diabetes on reproductive system in diabetic men.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Allium cepa (Onion) Seeds (AC) Extract on Histopathology of Testis in STZ-Induced Male Rats

Fallah

Mahabadi

et al. 2017

Int. J. Morphol.

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“…As indicated above, homologous species stem cell transplants have now been performed in several large animal models 42–47 , including our report that nonhuman primate SSCs could be frozen, thawed and transplanted to regenerate spermatogenesis with functional sperm 48 . Furthermore, we estimate from published reports and personal communications that testicular tissues or cells have been cryopreserved for several hundred patients worldwide 14,20,59 . Therefore, translation of the SSC transplantation technique to the clinic appears imminent.…”

Section: Stem Cell Therapies For Male Infertilitymentioning

confidence: 99%

Section: Stem Cell Therapies For Male Infertilitymentioning

confidence: 99%

“…2) 22,23,25,26,20,59 . Biopsied testicular tissues are typically cut into small pieces (1–9 mm 3 ); suspended in a DMSO based freezing medium and frozen at a controlled slow rate using a programmable freezing machine 64,22,61,23,25,20,59,27 . Some centers have reported using ethylene glycol based freezing medium instead of DMSO 57,60,65 and some centers have reported that viability of vitrified testicular tissue is similar to tissue frozen at a controlled slow rate 66–69 .…”

Section: Stem Cell Therapies For Male Infertilitymentioning

confidence: 99%

“…Each group reporting human SSC culture used different methods to isolate cells for culture, different feeder or matrix substrates, different growth factor cocktails and different methods to assess progress. To date, no human SSC culture method has be independently replicated by another group and this needs to happen to move the field forward (reviewed in Valli et al, 2015) 59 . Furthermore, while transplantation to regenerate spermatogenesis with functional sperm and offspring is the gold standard assay for rodent SSCs, there is not equivalent assay of human SSCs.…”

Section: Stem Cell Therapies For Male Infertilitymentioning

confidence: 99%

See 2 more Smart Citations

Experimental methods to preserve male fertility and treat male factor infertility

Gassei

Orwig

2016

Fertility and Sterility

Self Cite

114

103

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Infertility is a prevalent condition that has insidious impacts on the infertile individuals, their families and society that extend far beyond the inability to have a biological child. Lifestyle changes, fertility treatments and assisted reproductive technologies are available to help many infertile couples achieve their reproductive goals. All of these technologies require that the infertile individual is able to produce at least a small number of functional gametes (eggs or sperm). It is not possible for a person who does not produce gametes to have a biological child. This review focuses on the infertile man and describes several stem cell-based methods and gene therapy approaches that are in the research pipeline and may lead to new fertility treatment options for azoospermic men.

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Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells

Baert

Rombaut

Goossens

2017

Methods in Molecular Biology

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Organoid systems take advantage of the self-organizing capabilities of cells to create diverse multi-cellular tissue surrogates that constitute a powerful novel class of biological models. Clearly, the formation of a testicular organoid (TO) in which human spermatogenesis can proceed from a single-cell suspension would exert a tremendous impact on research and development, clinical treatment of infertility, and screening of potential drugs and toxic agents. Recently, we showed that primary adult and pubertal human testicular cells auto-assembled in TOs either with or without the support of a natural testis scaffold. These mini-tissues harboured both the spermatogonial stem cells and their important niche cells, which retained certain specific functions during long-term culture. As such, human TOs might advance the development of a system allowing human in vitro spermatogenesis. Here we describe the methodology to make scaffold-based and scaffold-free TOs.

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Stem Cell Therapies for Male Infertility: Where Are We Now and Where Are We Going?

Cited by 14 publications

References 164 publications

Protective Effect of Allium cepa (Onion) Seeds (AC) Extract on Histopathology of Testis in STZ-Induced Male Rats

Protective Effect of Allium cepa (Onion) Seeds (AC) Extract on Histopathology of Testis in STZ-Induced Male Rats

Experimental methods to preserve male fertility and treat male factor infertility

Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells

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