Stem Cell Niche-Radiobiological Response

Olivos, David J.; Sellamuthu, Rajendran; Singh, Pratibha; Vemula, Sasidhar; Pelus, Louis M.; Orschell, Christie M.; Kacena, Melissa A.

doi:10.1007/978-3-319-21702-4_5

Cited by 1 publication

(1 citation statement)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(31) described the deleterious effects of radiation on the BM vasculature in rodents as early as 1961 and Olivos et al . recently summarized the current state of knowledge in this regard (32). Also of importance is that formal analysis of the effects of acute radiation exposure on the human BM vasculature or human BM ECs has not been performed.…”

Section: Discussionmentioning

confidence: 99%

A Molecular Profile of the Endothelial Cell Response to Ionizing Radiation

Himburg¹,

Sasine²,

Yan³

et al. 2016

Radiation Research

View full text Add to dashboard Cite

Ionizing radiation exposure can cause acute radiation sickness (ARS) by damaging the hematopoietic compartment. Radiation damages quiescent hematopoietic stem cells (HSCs) and proliferating hematopoietic cells, resulting in neutropenia, thrombocytopenia and increased risk for long-term hematopoietic dysfunction and myelodysplasia. While some aspects of the hematopoietic response to radiation injury are intrinsic to hematopoietic cells, the recovery of the HSC pool and overall hematopoiesis is also dependent on signals from bone marrow endothelial cells (BM ECs) within the HSC vascular niche. The precise mechanisms through which BM ECs regulate HSC regeneration remain unclear. Characterization of the altered EC gene expression that occurs in response to radiation could provide a roadmap to the discovery of EC-derived mechanisms that regulate hematopoietic regeneration. Here, we show that 5 Gy total-body irradiation substantially alters the expression of numerous genes in BM ECs within 24 h and this molecular response largely resolves by day 14 postirradiation. Several unique and nonannotated genes, which encode secreted proteins were upregulated and downregulated in ECs in response to radiation. These results highlight the complexity of the molecular response of BM ECs to ionizing radiation and identify several candidate mechanisms that should be prioritized for functional analysis in models of hematopoietic injury and regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%