Stem cell impregnated nanofiber stent sleeve for on-stent production and intravascular delivery of paracrine factors

Hwang, Chao Wei; Johnston, Peter V.; Gerstenblith, Gary; Weiss, Robert G.; Tomaselli, Gordon F.; Bogdan, Virginia; Panigrahi, Asmi; Leszczynska, Aleksandra; Xia, Zhiyong

doi:10.1016/j.biomaterials.2015.02.047

Cited by 27 publications

(14 citation statements)

References 40 publications

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“…However, although a recent approach proposed the use of a nanofiber sleeve built on a stent, this is involved in the electro‐spun synthetic PLGA and mesenchymal stem cells (MSCs). [ 49 ] To our knowledge, there have not been any cases dealing with naturally derived resources for the EPC delivery as in ours, thus our approach is the first in which a cell‐derived extracellular matrix bound on a solid substrate was employed for the intravascular delivery of EPCs.…”

Section: Resultsmentioning

confidence: 99%

A Robustly Supported Extracellular Matrix Improves the Intravascular Delivery Efficacy of Endothelial Progenitor Cells

Park

Jeong

et al. 2021

Adv Funct Materials

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A major barrier in the multi‐purpose use of implantable materials is an incomplete integration with surrounding tissues, causing foreign body reactions, such as fibrous encapsulation and chronic inflammation. From its viewpoint, a universal method is suggested for cloaking any kind of substrate in specific cell‐made extracellular matrices (ECMs) via robust linkages for delivery of therapeutic cells. In addition, the feasibility of ECM‐coated substrates as endothelial progenitor cells (EPCs)‐laden coronary stent platform for endovascular implantation is investigated. Characteristics and stability of cell‐secreted ECMs anchored on a substrate are evaluated, and structural and componential features are revealed similar to those of native ECMs, with enough strength to enable practical uses. The in vitro experiments demonstrated that the ECM coating effectively supports the adhesion, proliferation, and viability of EPCs and has selectively opposite effects on smooth muscle cells. The in vivo experiments using a porcine coronary model supports that ECM‐coated stents enable endothelial regeneration and inhibit neointimal growth, and the cell‐laden form is more effective than other stents. These results will allow the preparation of tissue‐integrating materials containing cellular microenvironments and safely coat surfaces with cells to enable long‐term implantation and the continuous managing of chronic diseases.

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Section: Resultsmentioning

confidence: 99%

A Robustly Supported Extracellular Matrix Improves the Intravascular Delivery Efficacy of Endothelial Progenitor Cells

Park

Jeong

et al. 2021

Adv Funct Materials

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“…Hwang et al constructed a mesh-like nanofiber (NF) stent sleeve to harness stem cells on the stent to create an "intravascular paracrine factor factory" for paracrine factors (PFs) production. [87] In vitro experiment revealed that the NF sleeve could protect cells from washout and host immune response. Further study found that after coating with mesenchymal stem cells (MSCs), NF sleeves did not hamper MSCs, continued to secrete PFs and induced tubulogenesis in human endothelial cells.…”

Section: Endovascular Treatmentmentioning

confidence: 99%

Nanomaterials in Cerebrovascular Disease Diagnose and Treatment

Luo

Wang

2021

Part & Part Syst Charact

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The targeted drug systems based on nanomaterials include initiative targeting and passive targeting systems. [4] Passive targeting methods refer to the nanomaterial-targeted drug delivery systems after intravenous injection into the blood circulation. The nanomaterials will locate in a specific organ or disease site according to the physiological mechanism. Passive targeting is closely related to monocytes and macrophages in reticuloendothelial system. Active targeting is the selective delivery of drugs to specific sites by the high affinity between ligands and receptors on the surface of target cells. Compared with passive targeting, active targeting can improve specific targeting and reduce damage to normal tissues.Drug delivery is one of the important nanomaterials applications in CVD treatment. In the field of cerebral protection, numerous studies have reported very promising neuroprotective agents including antioxidants, calcium, and glutamate. [5] All of the agents have failed at clinical trials because of safety issues or low efficacy. [6] As the barrier between plasma and cerebrospinal fluid, blood brain barrier (BBB) is a major obstacle to prevent most drugs into brain. Application of nanomaterials could assist the drugs across BBB by targeted delivery systems.The BBB mainly contains two parts including the barrier between brain cells and plasma (formed by brain microvascular endothelial cells and glial cells) and the barrier between plasma formed by choroid plexus and cerebrospinal fluid. [7] These barriers can prevent certain harmful substances from the blood to enter brain tissue. At the same time, BBB can also prevent some useful brain-targeted drugs into brain lesions area. Nanomaterials were found to be alternative strategy to solve this problem. The probable mechanisms of nanomaterials across BBB involved endocytosis of brain microvascular endothelial cells (BMVECs), passive diffusion, transmembrane transport, and increment of solubility and fluidity of BMVEC membrane lipid. [8] In the field of CVD treatment, besides targeted drug delivery systems across BBB, there are still other directions for nanomaterials application. One of the major directions is thrombolytic drug delivery, such as delivering tissue-type plasminogen activator (t-PA) for thrombolysis. [9] The method has the advantage of accumulating drugs at the site of thrombus with high selectivity and biocompatibility which leading to minimal side effects. Another important application of nanomaterials is Cerebrovascular diseases (CVDs) are among the most serious diseases with high mortality and disability rates. The prevalent diagnosis and treatment methods of CVDs include imaging and interventional therapy. With the development of nanotechnology, large numbers of nanomaterials have been applied to the diagnosis and treatment of CVDs, mainly including carbon nanotubes, quantum dots, fullerenes, and dendrimers. In this review, the applications of nanomaterials in the field of diagnosis and treatment of CVDs, mainly including drug target de...

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“…Bae et al [142] made a three-dimensional bioprinting of an artificial trachea carrying in vitro cultured rabbit BM-MSCs and respiratory epithelial cells. In addition, in order to solve the problems of the cell washout and immune attack during MSC administration, Hwang et al [143] created an “intravascular paracrine factor (PF) factory” by harnessing stem cells on a stent using a nanofiber (NF) stent sleeve, thus providing a sheltered milieu for cells to continuously produce PFs on-stent. NF stent sleeves were created by covering stents with electrospun poly-lactic-co-glycolic acid nanofibers and were then uniformly coated with MSCs.…”

Section: Conclusion Questions and Perspectivesmentioning

confidence: 99%

Mesenchymal Stem Cells in Renal Fibrosis: The Flame of Cytotherapy

Zhuang

Yin

et al. 2019

Stem Cells International

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Renal fibrosis, as the fundamental pathological process of chronic kidney disease (CKD), is a pathologic extension of the normal wound healing process characterized by endothelium injury, myofibroblast activation, macrophage migration, inflammatory signaling stimulation, matrix deposition, and remodelling. Yet, the current method of treating renal fibrosis is fairly limited, including angiotensin-converting enzyme inhibition, angiotensin receptor blockade, optimal blood pressure control, and sodium bicarbonate for metabolic acidosis. MSCs are pluripotent adult stem cells that can differentiate into various types of tissue lineages, such as the cartilage (chondrocytes), bone (osteoblasts), fat (adipocytes), and muscle (myocytes). Because of their many advantages like ubiquitous sources, convenient procurement and collection, low immunogenicity, and low adverse effects, with their special identification markers, mesenchymal stem MSC-based therapy is getting more and more attention. Based on the mechanism of renal fibrosis, MSCs mostly participate throughout the renal fibrotic process. According to the latest and overall literature reviews, we aim to elucidate the antifibrotic mechanisms and effects of diverse sources of MSCs on renal fibrosis, assess their efficacy and safety in preliminarily clinical application, answer the controversial questions, and provide novel ideas into the MSC cellular therapy of renal fibrosis.

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Stem cell impregnated nanofiber stent sleeve for on-stent production and intravascular delivery of paracrine factors

Cited by 27 publications

References 40 publications

A Robustly Supported Extracellular Matrix Improves the Intravascular Delivery Efficacy of Endothelial Progenitor Cells

A Robustly Supported Extracellular Matrix Improves the Intravascular Delivery Efficacy of Endothelial Progenitor Cells

Nanomaterials in Cerebrovascular Disease Diagnose and Treatment

Mesenchymal Stem Cells in Renal Fibrosis: The Flame of Cytotherapy

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