Stem cell harvesting protocol research in autologous transplantation setting: Large volume vs. conventional cytapheresis

Balint, Bela; Ljubenov, Marika; Stamatović, Dragana; Todorović, Milena; Pavlović, Mirjana; Ostojić, Gordana; Jocić, Miodrag; Trkuljic, Miroljub

doi:10.2298/vsp0807545b

Cited by 18 publications

(40 citation statements)

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“…Finally, cells were cryopreserved using our original controlled-rate freezing procedure by optimized dimethyl sulfoxide (10% DMSO) and stored at -140 ± 5 °C (mechanical freezer) or at -196 °C (liquid nitrogen) and thawed immediately prior clinical use in a water bath at 37 ± 3 °C, as described previously 13,14 . The CD34 + cell quantity in harvest was determined with a flow cytometer (Beckman-Coulter, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Ova studija obuhvatila je ukupno 210 bolesnika (90 HL/120 MM) koji su bili lečeni primenom ATMĆ. Mobilizacija matičnih ćelija (MĆ) izvedena je pomoću stimulišućeg faktora granulocitnih kolonija (G-CSF) [10][11][12][13][14][15][16] μg/kg telesne mase (tm)] posle hemioterapije. Za dokazane "slabe-mobilizatore" izvedena je dodatna mobilizacija upotrebom G-CSF (16 μg/kgtm) uz dodatak Plerixafora (24 ili 48 mg).…”

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Influence of applied CD34+ cell dose on the survival of Hodgkin's lymphoma and multiple myeloma patients following autologous stem cell transplants

et al. 2020

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Background/Aim. Autologous stem cell transplants (ASCTs) improve the rate of overall survival (OS) in patients with hematological malignancies such as multiple myeloma (MM) after induction chemotherapy, aggressive non-Hodgkin's lymphomas (NHL), and relapsed, chemotherapy-sensitive Hodgkin's lymphoma (HL). The study aim was to evaluate influence of applied CD34 + cell quantity on clinical outcome, as well as early post-transplant and overall survival (OS) of HL and MM patients following ASCT. Methods. This study included a total of 210 patients (90 HL/120 MM) who underwent ASCT. Stem cell (SC) mobilization was accomplished by granulocyte-colony stimulating factor (G-CSF) 10-16 μg/kg body mass (bm) following chemotherapy. For proven poor mobilizers, mobilization with G-CSF (16 μg/kgbm) and Plerixafor (24 or 48 mg) was performed. To our best knowledge, it was the first usage of the Plerixafor in our country in the ASCT-setting. Harvesting was initiated merely at "cut-off-value" of CD34 + cells ≥ 20 × 10 6 /L in peripheral blood with "target-dose" of CD34 + cells ≥ 5 × 10 6 /kgbm in harvest. The CD34 + cell count and viability was determined using flow cytometry. Results. The majority of HL patients (76.7%) were infused with > 5.0 × 10 6 /kgbm CD34 + cells, while 68.3% of MM patients were treated by approximately 4.0-5.4 × 10 6 /kgbm CD34 + dose, respectively. Beneficial response (complete/partial remission) was achieved in 83.3% (HL) and 94.2% (MM) patients. Among parameters that influenced survival of HL patients with positive response to the therapy, multivariate analysis (pre-ASCT performance status, CD34 + cell quantity applied, rapid hematopoietic, i.e. lymphocyte and platelet recovery) indicated that higher CD34 + cell dose used, along with pre-ASCT performance status correlated with superior event-free survival (EFS) and OS following ASCT. In MM patients, multivariate analysis (renal impairment, infused CD34 + cell quantity, early platelet recovery) indicated that the number of CD34 + cells infused was the most important parameter that influenced both EFS and OS after ASCT. Conclusion. Data obtained in this study undoubtedly confirmed that CD34 + cell dose applied is an independent factor that may contribute to superior clinical outcome and OS of HL and MM patients following ASCT.

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Section: Methodsmentioning

confidence: 99%

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Influence of applied CD34+ cell dose on the survival of Hodgkin's lymphoma and multiple myeloma patients following autologous stem cell transplants

et al. 2020

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“…For autologous SC-transplants, cryopreservation was performed according to our original five-step controlled-rate freezing protocol (with compensation of the released fusion heat), using dimethyl sulfoxide (DMSO; 10% final concentration) by Planer 560-16 equipment (Planer Products Ltd, UK), as it was earlier described [13][14][15] .…”

Section: Cell Quantifications Techniquesmentioning

confidence: 99%

Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Balint¹,

Stanojević

Todorović

et al. 2017

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Background/Aim. Stem cells (SCs) guarantee complete/long-term bone marrow (BM) repopulation after SCtransplants. The aim of the study was to evaluate absolute count of total SCs (determined by ISHAGE-sequential-gating protocol -SC ish ) and relative frequency of immature CD34 + /CD90 + (CD90 + SC ish ) subset in peripheral blood (PB) as predictive factors of mobilization and apheresis product (AP) quality. Methods. Mobilization included chemotherapy and granulocyte-growth-factor (G-CSF). Harvesting was performed by Spectra-Optia-IDL-system. The SCs ish were determined as a constitutional part of CD34 + cells in the "stemcell-region" using FC-500 flow-cytometer. In this study, the original ISHAGE-sequential-gating protocol was modified by introduction of anti-CD90-PE monoclonal-antibody into the analysis of CD90 expression on SC ish (CD90 + SC ish ). The results were presented as a percentage of SC ish per nucleatedcell count, absolute SC ish count in μL of the PB or the AP, percentage of the CD90 + SC ish expressed to SC ish and absolute CD90 + SC ish count in μL of the PB or the AP. Results. The absolute count of total SC ish and CD90 + SC ish was significantly higher (p = 0.0007 and p = 0.0266, respectively) in the AP than in the PB samples. The CD90 + SC ish /total SC ish indexes from PB were higher than indexes from the AP (p = 0.039). Key words: stem cells; hematopoietic stem cell transplantation; bone marrow; flow cytometry; multiple myeloma; antineoplastic combined chemotherapy protocols.Apstrakt Uvod/Cilj. Matične ćelije (MĆ) obezbeđuju kompletnu/dugotrajnu repopulaciju kostne srži (KS) posle trans-plantacije. Cilj ove studije bila je procena apsolutnog broja ukupnih MĆ (utvrđena protokolom "ISHAGE-sequentialgating" -Stem-Cell ish [SC ish ]) i relativne učestalosti primitivnih podipova CD34 + /CD90 + (CD90 + SC ish ) u perifernoj krvi (PK) Ključne reči: ćelije, matične; transplantacija hematopoeznih matičnih ćelija; kostna srž; citometrija, protočna; multipli mijelom; lečenje kombinovanjem antineoplastika, protokoli.

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“…Hematological diseases have so far been the most common indication of this treatment modality; it has been less often used for nonmalignant disorders. Nowadays BM and peripheral blood (PB) derived SC transplants are more common in adult allogeneic or autologous setting [2,[6][7][8]. Umbilical cord blood (UCB) transplants have provided hopeful results in pediatric setting mainly when a matched unrelated SC donor is not obtainable [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Stem Cell-Based (Auto)Grafting: From Innovative Research Toward Clinical Use in Regenerative Medicine

Balint¹,

Obradović²,

Todorović³

et al. 2013

Stem Cell Biology in Normal Life and Diseases

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Stem cell harvesting protocol research in autologous transplantation setting: Large volume vs. conventional cytapheresis

Cited by 18 publications

References 17 publications

Influence of applied CD34+ cell dose on the survival of Hodgkin's lymphoma and multiple myeloma patients following autologous stem cell transplants

Influence of applied CD34+ cell dose on the survival of Hodgkin's lymphoma and multiple myeloma patients following autologous stem cell transplants

Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Stem Cell-Based (Auto)Grafting: From Innovative Research Toward Clinical Use in Regenerative Medicine

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