Stem cell factor and IL-6 do not promote complete maturation of human cultured mast cells from umbilical cord blood cells: an ultrastructural study

Matsushima, Yoichiro; Ishikawa, Osamu; Kurosawa, Motohiro

doi:10.1016/s0923-1811(00)00076-1

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…Interestingly, the study showed the expression of functional CXCR2 on the mast cells after 4 weeks of differentiation. As the ultrastructural investigation at our laboratory [35]showed SCF and IL-6 may not promote complete maturation of cultured human mast cells from umbilical cord blood cells, the mast cells used in the present study might be analogous to differentiating progenitors rather than fully matured mast cells. Therefore, it would be of interest to examine a time course of CXCR1 and CXCR2 expression on the cultured mast cells.…”

Section: Discussionmentioning

confidence: 93%

Expression of the Interleukin-8 Receptors CXCR1 and CXCR2 on Cord-Blood-Derived Cultured Human Mast Cells

Inamura¹,

Kurosawa²,

Okano³

et al. 2002

Int Arch Allergy Immunol

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Background: An increase in mast cell number at sites of inflamed tissues has been observed. However, the expression of CXC chemokine receptors on human mast cells is poorly understood. Methods: Cultured human mast cells were raised from human umbilical cord blood cells in the presence of stem cell factor and interleukin-6 (IL-6). The expression of surface chemokine receptors on the mast cells was analyzed by flow cytometry and that of mRNA was examined by the method of reverse transcriptase-polymerase chain reaction (RT-PCR). As functional assays for the receptors, mast cell migration was examined by a microchemotaxis assay and changes in the cytosolic free intracellular calcium concentrations ([Ca²⁺]_i) was measured using fura-2-loaded mast cells, respectively. Results: Expression of IL-8 receptors CXCR1 and CXCR2 was demonstrated by flow cytometry and of both mRNA by RT-PCR; however, CC chemokine receptors including CCR3 were not expressed on cord-blood-derived cultured human mast cells. IL-8 and its homologues showed chemotactic activity toward them in a dose-dependent manner, and IL-8 induced a dose-dependent rapid and transient increase in [Ca²⁺]_i in the mast cells. Conclusions: Our results suggest the surface expression of functional CXCR1 and CXCR2 on cord-blood-derived cultured human mast cells.

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Section: Discussionmentioning

confidence: 93%

Expression of the Interleukin-8 Receptors CXCR1 and CXCR2 on Cord-Blood-Derived Cultured Human Mast Cells

Inamura¹,

Kurosawa²,

Okano³

et al. 2002

Int Arch Allergy Immunol

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“…1d). This is in contrast to the cells derived from cord blood, which retain the morphological characteristics of immature mast cells, including multilobate nuclei, even after 16 -20 weeks in culture (27). Culturing HuMCs from CD34 ϩ progenitor cells has distinct advantages over previously described systems as the cells are not exposed to digestive enzymes and do not require purification steps.…”

Section: Models For Fcri-dependent Signaling In Human Mast Cellsmentioning

confidence: 93%

FcεRI-Dependent Signaling Pathways in Human Mast Cells

Tkaczyk

Gilfillan

2001

Clinical Immunology

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“…The combination of hyper-IL-6, SCF and GM-CSF could promote the differentiation of dendritic cells, thus stimulating the resting T cells against the processed antigen (Bernhard et al, 2000). Furthermore, IL-6 and SCF could partly promote the maturation of human cultured mast cells, and other factors may be involved in this process (Matsushima et al, 2000). In this paper, IL-6 and SCF had the same up-regulated trends, when lncRNA CRNG was overexpressed.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine

Huang

et al. 2019

Front. Pharmacol.

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Our previous study has showed that a novel gene is differentially expressed in the liver of cyadox-fed piglets, but its sequence and function are unknown. Here, rapid amplification of cDNA ends (RACE) and bioinformatics analysis showed that the novel gene is 953 bp without protein-coding ability and locates in chromosome 11. Hence, we identified the novel gene as long non-coding RNA (lncRNA) and named it cyadox-related novel gene (CRNG). Fluorescence in situ hybridization (FISH) showed that CRNG mainly distributes in cytoplasm. Moreover, microarray assay in combination with CRNG interference and overexpression showed that the differential genes such as ANPEP, KITLG, STAT5A, FOXP3, miR-451, IL-2, IL-10, IL-6, and TNF-α are mainly involved in viral and pathogens infection and the immune-inflammatory responses in PK-15 cells. This work reveals that CRNG might play a role in preventing the host from being infected by pathogens and viruses and exerting immune regulatory effects in the cytoplasm, which may be involved in prophylaxis of cyadox in piglets.

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Stem cell factor and IL-6 do not promote complete maturation of human cultured mast cells from umbilical cord blood cells: an ultrastructural study

Cited by 6 publications

References 16 publications

Expression of the Interleukin-8 Receptors CXCR1 and CXCR2 on Cord-Blood-Derived Cultured Human Mast Cells

Expression of the Interleukin-8 Receptors CXCR1 and CXCR2 on Cord-Blood-Derived Cultured Human Mast Cells

FcεRI-Dependent Signaling Pathways in Human Mast Cells

Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine

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