Stem Cell Differentiation into Cardiomyocytes: Current Methods and Emerging Approaches

Afjeh-Dana, Elham; Naserzadeh, Parvaneh; Moradi, Elham; Hosseini, Nasrin; Seifalian, Alexander M.; Ashtari, Behnaz

doi:10.1007/s12015-021-10280-1

Cited by 15 publications

(17 citation statements)

References 215 publications

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“…They have several advantages over bone marrow MSCs, such as higher proliferation, easy harvesting, decreased risk of unsuccessful grafting, enhancement of angiogenesis, and anti-fibrotic effects. Moreover, they possess low immunogenicity ( Fonoudi et al, 2015 ; Afjeh-Dana et al, 2022 ). Guo and others transplanted UC-SCs sheets post-MI in a mouse model.…”

Section: The Shortening Gap Between Preclinical Research and Clinical...mentioning

confidence: 99%

Advances in the design, generation, and application of tissue-engineered myocardial equivalents

Bernava,

Iop

2023

Front. Bioeng. Biotechnol.

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Due to the limited regenerative ability of cardiomyocytes, the disabling irreversible condition of myocardial failure can only be treated with conservative and temporary therapeutic approaches, not able to repair the damage directly, or with organ transplantation. Among the regenerative strategies, intramyocardial cell injection or intravascular cell infusion should attenuate damage to the myocardium and reduce the risk of heart failure. However, these cell delivery-based therapies suffer from significant drawbacks and have a low success rate. Indeed, cardiac tissue engineering efforts are directed to repair, replace, and regenerate native myocardial tissue function. In a regenerative strategy, biomaterials and biomimetic stimuli play a key role in promoting cell adhesion, proliferation, differentiation, and neo-tissue formation. Thus, appropriate biochemical and biophysical cues should be combined with scaffolds emulating extracellular matrix in order to support cell growth and prompt favorable cardiac microenvironment and tissue regeneration. In this review, we provide an overview of recent developments that occurred in the biomimetic design and fabrication of cardiac scaffolds and patches. Furthermore, we sift in vitro and in situ strategies in several preclinical and clinical applications. Finally, we evaluate the possible use of bioengineered cardiac tissue equivalents as in vitro models for disease studies and drug tests.

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Section: The Shortening Gap Between Preclinical Research and Clinical...mentioning

confidence: 99%

Advances in the design, generation, and application of tissue-engineered myocardial equivalents

Bernava,

Iop

2023

Front. Bioeng. Biotechnol.

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“…Umbilical cord stem cells (UCB-SCs) have several advantages over the BM-MSCs, such as higher proliferation, easy harvesting, decreased risk of unsuccessful grafting, enhancement of angiogenesis, and anti-fibrotic effects. Moreover, they have lower immunogenicity [ 71 , 72 ]. Therefore, it is not surprising that UCB-SCs are considered one of the potential cell sources for MI therapy.…”

Section: Cell-based Therapies For MImentioning

confidence: 99%

Cell-Based and Selected Cell-Free Therapies for Myocardial Infarction: How Do They Compare to the Current Treatment Options?

Csöbönyeiová

Beerová

Klein

et al. 2022

IJMS

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Because of cardiomyocyte death or dysfunction frequently caused by myocardial infarction (MI), heart failure is a leading cause of morbidity and mortality in modern society. Paradoxically, only limited and non-curative therapies for heart failure or MI are currently available. As a result, over the past two decades research has focused on developing cell-based approaches promoting the regeneration of infarcted tissue. Cell-based therapies for myocardial regeneration include powerful candidates, such as multipotent stem cells (mesenchymal stem cells (MSCs), bone-marrow-derived stem cells, endothelial progenitor cells, and hematopoietic stem cells) and induced pluripotent stem cells (iPSCs). These possess unique properties, such as potency to differentiate into desired cell types, proliferation capacity, and patient specificity. Preclinical and clinical studies have demonstrated modest improvement in the myocardial regeneration and reduced infarcted areas upon transplantation of pluripotent or multipotent stem cells. Another cell population that need to be considered as a potential source for cardiac regeneration are telocytes found in different organs, including the heart. Their therapeutic effect has been studied in various heart pathologies, such as MI, arrhythmias, or atrial amyloidosis. The most recent cell-free therapeutic tool relies on the cardioprotective effect of complex cargo carried by small membrane-bound vesicles—exosomes—released from stem cells via exocytosis. The MSC/iPSC-derived exosomes could be considered a novel exosome-based therapy for cardiovascular diseases thanks to their unique content. There are also other cell-free approaches, e.g., gene therapy, or acellular cardiac patches. Therefore, our review provides the most recent insights into the novel strategies for myocardial repair based on the regenerative potential of different cell types and cell-free approaches.

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“…1 illustrates the classification of SCs based on their potency and origin. ESCs are among the pluripotent SCs that are extracted from 3-5-day-old blastocyst embryos and are found in the inner cell mass before implantation [ [3] , [4] , [5] , [6] ]. Umbilical cord SCs are extracted from the umbilical cord of the fetus.…”

Section: Introductionmentioning

confidence: 99%

The impact of acute and chronic aerobic and resistance exercise on stem cell mobilization: A review of effects in healthy and diseased individuals across different age groups

Li,

Chen,

Mohammad Sajadi

et al. 2024

Regenerative Therapy

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Stem Cell Differentiation into Cardiomyocytes: Current Methods and Emerging Approaches

Cited by 15 publications

References 215 publications

Advances in the design, generation, and application of tissue-engineered myocardial equivalents

Advances in the design, generation, and application of tissue-engineered myocardial equivalents

Cell-Based and Selected Cell-Free Therapies for Myocardial Infarction: How Do They Compare to the Current Treatment Options?

The impact of acute and chronic aerobic and resistance exercise on stem cell mobilization: A review of effects in healthy and diseased individuals across different age groups

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