Stem cell ageing of the root apical meristem of Arabidopsis thaliana

Wein, Annika; Gac, Anne-Laure Le; Laux, Thomas

doi:10.1016/j.mad.2020.111313

Cited by 8 publications

(5 citation statements)

References 52 publications

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“…QC daughter cells usually become CSCs (Cruz-Ramirez et al ., 2013); however, clonal analysis shows that QC cells can potentially replace all stem cells in the meristem ( Kidner et al , 2000 ). Cell divisions in the QC accelerate upon meristem aging, disturbing the stem cell niche structure ( Timilsina et al , 2019 ; Wein et al , 2020 ). It is unclear whether meristem aging is part of an inherent developmental program or is the consequence of accumulating stress.…”

Section: Introductionroot Stem Cell Niche Structure and Dynamicsmentioning

confidence: 99%

Mechanisms of stress response in the root stem cell niche

Ubogoeva

Zemlyanskaya

et al. 2021

Journal of Experimental Botany

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As plants are sessile organisms unable to escape from environmental hazards, they need to adapt for survival. The stem cell niche in the root apical meristem is particularly sensitive to DNA damage induced by environmental stresses such as chilling, flooding, wounding, UV, and irradiation. DNA damage has been proven to cause stem cell death, with stele stem cells being the most vulnerable. Stress also induces the division of quiescent center cells. Both reactions disturb the structure and activity of the root stem cell niche temporally; however, this preserves root meristem integrity and functioning long-term. Plants have evolved many mechanisms that ensure stem cell niche maintenance, recovery, and acclimation, allowing them to survive in a changing environment. Here, we give an overview of the cellular and molecular aspects of stress responses in the root stem cell niche.

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Section: Introductionroot Stem Cell Niche Structure and Dynamicsmentioning

confidence: 99%

Mechanisms of stress response in the root stem cell niche

Ubogoeva

Zemlyanskaya

et al. 2021

Journal of Experimental Botany

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“…We found that genes involved in maintaining the quiescent central niche were included in this pattern, such as PLT1/5 , and WOX5 (Fig. 3h), indicating that these 17 genes might promote the acquisition of cell pluripotency during the redifferentiation process 49,50 . Taken together, here we demonstrate that the 75 canonical regeneration-related genes were specifically expressed at certain stages during shoot regeneration, and these findings shed light on the shoot regeneration mechanisms.…”

Section: Resultsmentioning

confidence: 85%

Single-cell transcriptome reveals the redifferentiation trajectories of the early stage of de novo shoot regeneration in Arabidopsis thaliana

Liu

et al. 2022

Preprint

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De novo shoot regeneration from a callus plays a crucial role in both plant biotechnology and the fundamental research of plant cell totipotency. Recent studies have revealed many regulatory factors involved in this developmental process. However. our knowledge of the cell heterogeneity and cell fate transition during de novo shoot regeneration is still limited. Here, we performed time-series single-cell transcriptome experiments to reveal the cell heterogeneity and redifferentiation trajectories during the early stage of de novo shoot regeneration. Based on the single-cell transcriptome data of 35,669 cells at five-time points, we successfully determined seven major cell populations in this developmental process and reconstructed the redifferentiation trajectories. We found that all cell populations resembled root identities and undergone gradual cell-fate transitions. In detail, the totipotent callus cells differentiated into pluripotent QC-like cells and then gradually developed into less differentiated cells that have multiple root-like cell identities, such as pericycle-like cells. According to the reconstructed redifferentiation trajectories, we discovered that the canonical regeneration-related genes were dynamically expressed at certain stages of the redifferentiation process. Moreover, we also explored potential transcription factors and regulatory networks that might be involved in this process. The transcription factors detected at the initial stage, QC-like cells, and the end stage provided a valuable resource for future functional verifications. Overall, this dataset offers a unique glimpse into the early stages of de novo shoot regeneration, providing a foundation for a comprehensive analysis of the mechanism of de novo shoot regeneration.

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“…This stability and the normal development of roots hinge on the consistent arrangement of QC cells and surrounding stem cells in the root apical meristem. While other plant species often exhibit more complex root structures than Arabidopsis thaliana , the straightforward organization of Arabidopsis roots has made it an ideal model for plant stem cell research ( Timilsina et al., 2019 ; Wein et al., 2020 ).…”

Section: Stem Cells Of the Root Apical Meristemmentioning

confidence: 99%

Maintenance of stem cell activity in plant development and stress responses

Zhang,

Mu,

Zhang

et al. 2023

Front. Plant Sci.

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Stem cells residing in plant apical meristems play an important role during postembryonic development. These stem cells are the wellspring from which tissues and organs of the plant emerge. The shoot apical meristem (SAM) governs the aboveground portions of a plant, while the root apical meristem (RAM) orchestrates the subterranean root system. In their sessile existence, plants are inextricably bound to their environment and must adapt to various abiotic stresses, including osmotic stress, drought, temperature fluctuations, salinity, ultraviolet radiation, and exposure to heavy metal ions. These environmental challenges exert profound effects on stem cells, potentially causing severe DNA damage and disrupting the equilibrium of reactive oxygen species (ROS) and Ca2+ signaling in these vital cells, jeopardizing their integrity and survival. In response to these challenges, plants have evolved mechanisms to ensure the preservation, restoration, and adaptation of the meristematic stem cell niche. This enduring response allows plants to thrive in their habitats over extended periods. Here, we presented a comprehensive overview of the cellular and molecular intricacies surrounding the initiation and maintenance of the meristematic stem cell niche. We also delved into the mechanisms employed by stem cells to withstand and respond to abiotic stressors.

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Stem cell ageing of the root apical meristem of Arabidopsis thaliana

Cited by 8 publications

References 52 publications

Mechanisms of stress response in the root stem cell niche

Mechanisms of stress response in the root stem cell niche

Single-cell transcriptome reveals the redifferentiation trajectories of the early stage of de novo shoot regeneration in Arabidopsis thaliana

Maintenance of stem cell activity in plant development and stress responses

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