Stem cell activity of porcine c-kit+ hematopoietic cells

Guern, Annie C Le; Giovino, Maria; Abe, Masahiro; Theodore, Pierre; Qi, Jin; Down, Julian D.; Sachs, David H.; Sykes, Megan; Yang, Yong‐Guang

doi:10.1016/s0301-472x(03)00197-8

Cited by 14 publications

(21 citation statements)

References 43 publications

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“…In the swine, the stem cell activity of CD117 + cells have been previously evaluated by using SCF to activate their stem cell potential to differentiate into various cellular lineages (20,21). Although a swine CD117 Mab has been developed and characterized (24), the species specificity of the Mab and xenotransplantation of CD117 + cells have not been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, they may have similar characteristics to the human and potentially could be used in experimental transplantation models for studying the pathogenesis and treatments of some human diseases. In this regard, Le Guern et al (20) reported on the effect of long-term engraftment of swine stem cell factor (SCF)-positive cells in immunodeficient mice. However, because there were no species-specific Mabs readily available against swine CD117, researchers have tended to use the stem cell factor as a detectable stem cell differentiation marker and not CD117 (21).…”

Section: Introductionmentioning

confidence: 99%

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Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody

Ohshima¹,

Mori²,

Shigenari³

et al. 2014

BST

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody

Ohshima¹,

Mori²,

Shigenari³

et al. 2014

BST

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“…Although CD34 is a widely used marker for HSC in rodents and humans, there is not yet a commercially available anti-CD34 antibody for pigs. However, c-kit+ expression is known to have a strong correlation with stem cell activity and can therefore be reliably used as a stem cell marker in pigs [Le Guern et al, 2003;Dor et al, 2004]. By using both BM and spleen as sources of HSC, it has been possible to compare the results, not only between these two sources, but also between splenic HSC and the MSC described by others [Makino et al, 1999;Tomita et al, 1999;Liu et al, 2003;Rangappa et al, 2003].…”

Section: Discussionmentioning

confidence: 99%

“…The cells were resuspended in culture medium consisting of RPMI 1640, 15% fetal bovine serum, penicillin (1 U/ml), streptomycin (1 mg/ml), gentamicin (0.05 mg/ ml), amphotericin B (0.34 g/ml) (all from Gibco), Hepes buffer (10 m M; Mediatech) and glutamine (2 m M; Gibco). The cells were incubated with 1: 40 porcine biotinylated recombinant porcine stem cell factor (c-kit ligand) (BioTransplant, Charlestown, Mass., USA) [Le Guern et al, 2003], then with magnetic cell separation (MACS) anti-biotin magnetic microbeads (MiltenyiBiotec, Auburn, Calif., USA), and separated over MACS LS columns (MiltenyiBiotec) with the negative fraction being retained for use in the c-kit-depleted (c-kit-) cultures. The c-kit+ cells were then eluted for use as HSC.…”

Section: Primary Culture Of Porcine C-kit Cellsmentioning

confidence: 99%

“…Although CD34 is often used as a marker of the earliest HSC, it is also known that, in the sequence of events associated with hematopoietic differentiation, porcine cells expressing c-kit (c-kit+) contain pluripotent HSC as well [Le Guern et al, 2003]. Through the use of the cobblestone area-forming cell (CAFC) assay, an assay that defi nes the presence of early HSC [Ploemacher et al, 1989;Breems et al, 1994], we document-ed an equal frequency of these earliest stem cells in BM and spleen [Dor et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

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Lack of Cardiac Differentiation in c-kit-Enriched Porcine Bone Marrow and Spleen Hematopoietic Cell Cultures Using 5-Azacytidine

Ramirez

McMorrow

Sanderson

et al. 2005

Cells Tissues Organs

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The adult spleen is a source of early hematopoietic stem cells (HSC). We therefore studied whether culturing spleen or bone marrow (BM) HSC in medium containing 5-azacytidine could induce a cardiac phenotype. c-kit enrichment and depletion of adult pig spleen and BM mononuclear cells were obtained by magnetic bead separation using biotinylated pig stem cell factor (c-kit ligand). Cells were incubated with 5-azacytidine for 24 h and refreshed with 5-azacytidine-free medium every 48 h. Western blot was used to detect cardiac troponin and myosin heavy chains. Although 5-azacytidine treatment led to the formation of ball-like cell clusters in both c-kit-enriched populations, these clusters showed no rhythmic contractions (beating), as observed by others. Furthermore, neither cardiac troponin nor myosin was detected in cells derived from either source. Our methodology and treatment with 5-azacytidine did not induce cardiac gene expression in porcine HSC derived from either pig spleen or BM.

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Application of xenogeneic stem cells for induction of transplantation tolerance: present state and future directions

Yang

2004

Springer Semin Immun

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Xenotransplantation using pig organs provides a possible solution to the severe shortage of allogeneic organ donors, one of the major limiting factors in clinical transplantation. However, because of the greater antigenic differences that exist between different species than within a species, the immune response to xenografts is much more vigorous than to allografts. Thus, tolerance induction is essential to the success of clinical xenotransplantation. Tolerance induced by mixed hematopoietic chimerism across the MHC barrier is remarkably robust, but its ability to induce tolerance across highly disparate xenogeneic barriers remains poorly studied. None of the current available regimens of host conditioning, which permit hematopoietic stem cell engraftment and chimerism induction in allogeneic or closely related (concordant) xenogeneic combinations, has been demonstrated to be effective in establishing porcine hematopoietic chimerism in a discordant xenogeneic species. Unlike bone marrow transplantation within the same species, the innate immune system and the species specificity of cytokines and adhesion molecules essential to hematopoiesis pose formidable obstacles to the establishment of donor hematopoiesis across discordant xenogeneic barriers. The genetic incompatibility between species may also impede xenograft tolerance induction by mixed chimerism. While we remain far from achieving tolerance in clinical xenotransplantation, recent studies using a transgenic mouse model have proven the principle that mixed hematopoietic chimerism may induce mouse and human T cell tolerance to porcine xenografts. This review article focuses on the barriers to porcine hematopoietic engraftment in highly disparate xenogeneic species and the possible application of mixed hematopoietic chimerism to xenograft tolerance induction.

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Stem cell activity of porcine c-kit+ hematopoietic cells

Cited by 14 publications

References 43 publications

Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody

Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody

Lack of Cardiac Differentiation in c-kit-Enriched Porcine Bone Marrow and Spleen Hematopoietic Cell Cultures Using 5-Azacytidine

Application of xenogeneic stem cells for induction of transplantation tolerance: present state and future directions

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