In this paper, we obtain monotonicity of Steklov eigenvalues on graphs which as a special case on trees extends the results of He-Hua [arXiv: 2103.07696] to higher Steklov eigenvalues and gives affirmative answers to two problems proposed in He-Hua [arXiv: 2103.07696]. As applications of the monotonicity of Steklov eigenvalues, we obtain some estimates for Steklov eigenvalues on trees generalizing the isodiametric estimate for the first positive Steklov eigenvalues on trees in He-Hua [arXiv:2011.11014].