Steinernema puertoricensis n. sp. (Rhabditida: Steinernematidae), una nueva especie de nematodo entomopatógeno de Puerto Rico

Román, Jessé; Figueroa, Wilfredo

doi:10.46429/jaupr.v78i3-4.4285

Cited by 27 publications

(4 citation statements)

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“…Indeed, in the Steinernema genus, the species grouping seems to be already well established. Among the 17 Steinernema species described (Poinar, 1990;Doucet & Doucet, 1990;Nguyen & Smart, 1992;Cabanillas et al 1994;Mracek, 1994;Mracek et al 1994;Roman & Figueroa, 1994), we have tested 9 of them (Grenier et al 1995) and our results are completely in agreement with this current grouping.…”

Section: Discussionsupporting

confidence: 87%

“…At this level, species identification has been a formidable and a time-consuming task -especially for Heterorhabditis -even for well-qualified taxonomists. For this reason, only 6 species of Heterorhabditis have been clearly recognized (Poinar, 1990;Poinar et al 1992;Stock, 1993;Gardner, Stock & Kaya, 1994), while 17 species have yet been described in the genus Steinernema (Poinar, 1990;Doucet & Doucet, 1990;Nguyen & Smart, 1992;Mracek et al 1994;Roman & Figueroa, 1994), including Steinernema kraussei (Mracek, 1994), Steinernema longicaudum and Steinernema serratum (Cabanillas, Poinar & Raulston, 1994). Still now, many isolates remain unassigned at the species level and the different strains inside a particular species cannot be distinguished.…”

Section: Introductionmentioning

confidence: 99%

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Use of species-specific satellite DNAs as diagnostic probes in the identification of Steinernematidae and Heterorhabditidae entomopathogenic nematodes

Grenier¹,

Bonifassi²,

Abad³

et al. 1996

Parasitology

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Three satellite DNAs previously isolated from the entomopathogenic nematodes Steinernema carpocapsae, Heterorhabditis bacteriophora and Heterorhabditis indicus give hybridization signals only with the S. carpocapsae, H. bacteriophora and H. indicus populations tested, indicating that these satellite sequences are species-specific. Because of their reiteration and their variabilities, we have shown that these sequences are able to discriminate at the interspecific level between the Steinernema and Heterorhabditis species, but also at the intraspecific level between S. carpocapsae strains. Furthermore, in simple squashed nematode experiments, we are able to unambiguously identify S. carpocapsae, H. bacteriophora and H. indicus populations. This last procedure is effective even on a single infective juvenile, with the main advantage that it avoids time-consuming DNA extractions.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Use of species-specific satellite DNAs as diagnostic probes in the identification of Steinernematidae and Heterorhabditidae entomopathogenic nematodes

Grenier¹,

Bonifassi²,

Abad³

et al. 1996

Parasitology

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“…montkolum Stock, Choo & Kaya, 1997S. neocurtillae Nguyen & Smart, 1992 S. oregonense Liu & Berry, 1996 S. puertoricense Roman & Figueroa, 1994S. rarum (de Doucet, 1986) Mamiya, 1988 syn.…”

Section: Family Steinernematidae Chitwood and Chitwood 1937mentioning

confidence: 99%

“…1.6 (1.4-1.6) Poinar, 1985Kozodoi, 1984Nguyen & Smart, 1990Roman & Figueroa, 1994Nguyen & Smart, 1992aShen & Wang, 1992Jian et al, 1997Stock et al, 1997Liu & Berry, 1996bPoinar, 1988Poinar, 1979de Doucet & Doucet, 1990Cabanillas et al, 1994Nguyen & Smart, 1992aTallosi et al, 1995Mamiya, 1988Mracek et al, 1994Nguyen & Smart, 1992aMracek, 1994 de Doucet, 1986Nguyen & Smart, 1994 Figures show means and range (measurements in jtm). D = distance from anterior end to excretory pore divided by oesophagus length; SW = spicule length divided by anal body width; GS = gubernaculum length divided by spicule length; MUC = mucron: a = absent, p = present, N = number of specimens measured;data not available.…”

Section: Key To Heterorhabditis Species (Devised By P Stock)mentioning

confidence: 99%

Biosystematics of entomopathogenic nematodes: current status, protocols and definitions

Hominick¹,

Briscoe²,

et al. 1997

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COST Action 819: Entomopathogenic nematodes, supercedes Action 812: Cold active lines of insect parasitic nematodes in Agriculture and Biotechnology. It functions in the field of Agriculture and Biotechnology and began in July 1994 and will end in May, 1999. The main objective is to combine interrelated European expertise to increase the use of entomopathogenic nematodes (EPNs) in integrated pest management and to reduce the need for chemical control. Coordination of the Action is the responsibility of a management committee in accordance with a Memorandum of Understanding, which has been signed by representatives of 17 countries: Austria, Belgium, Czech Republic, Finland, France, Germany, Hungary, Ireland, Italy, The Netherlands, Norway, Poland, Portugal, Spain, Sweden, Switzerland and the UK. A research institute in Israel is also participating. Over 40 research institutions and 10 commercial companies participate in Action 819. The EU funding meets the costs related to coordination including the scientific secretariat, workshops, management committee and working group meetings, publications, short term scientific missions and evaluations.

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Steinernema costaricense n. sp. and S. puntauvense n. sp. (Rhabditida: Steinernematidae), two new entomopathogenic nematodes from Costa Rica

2007

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Steinernema costaricense n. sp. and S. puntauvense n. sp. were recovered during a survey for native entomopathogenic nematodes in Costa Rica. Morphological data, molecular (28S rDNA sequence data) studies and cross-hybridisation tests were used for diagnostic and identification purposes. Additionally, 28S rDNA sequence data were used to assess the evolutionary relationships of the new species with other Steinernema spp. Morphological diagnostic features for S. costaricense n. sp. include: the body size of the infective juvenile (av. 1,696); the presence of protruding 'horn-like' cephalic papillae; the position of the excretory pore in the infective juvenile (av. 77 microm) and the first generation male (av. 117 microm); the D% value of the infective juvenile (av. 53) and the first generation male (av. 56); the E% value of the infective juvenile (av. 85); and the morphology of the spicules and gubernaculum of the first generation male. Diagnostic traits for S. puntauvense n. sp. are: the position of the excretory pore in the infective juveniles (av. 25 microm); the shape and size of the spicules and gubernaculum of the first generation male; and the shape of the tail of the first generation female. In addition to these traits, 28S rDNA sequences analysis and hybridisation tests showed that both new Steinernema species are distinct and unique entities.

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Steinernema puertoricensis n. sp. (Rhabditida: Steinernematidae), una nueva especie de nematodo entomopatógeno de Puerto Rico

Cited by 27 publications

References 0 publications

Use of species-specific satellite DNAs as diagnostic probes in the identification of Steinernematidae and Heterorhabditidae entomopathogenic nematodes

Use of species-specific satellite DNAs as diagnostic probes in the identification of Steinernematidae and Heterorhabditidae entomopathogenic nematodes

Biosystematics of entomopathogenic nematodes: current status, protocols and definitions

Steinernema costaricense n. sp. and S. puntauvense n. sp. (Rhabditida: Steinernematidae), two new entomopathogenic nematodes from Costa Rica

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