Steinernema lamjungense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Lamjung district, Nepal

Abstract

A new species of entomopathogenic nematode, Steinernema lamjungense n. sp., was recovered from soil samples collected from Tarku, Lamjung district of Nepal, during a survey in 2007. The analysis of ITS-rDNA sequence placed S. lamjungense n. sp. in the arenarium-glatseri-karii-longicaudum group. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of different stages and by characterisations and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. For infective juveniles, the new species can be recognised by medium-long body length of 832 (690-950) mu m, pharynx length of 127 (110-142) mu m, H% = 61(55-69) and E% = 79 (69-97). The lateral field pattern is 2, 6, 7, 8, 3, 2. The males have well curved, yellowish-brown spicules with a prominent velum. The spicule terminus is blunt with a longitudinal ventral notch. Spicule length is 87 (81-94) mu m and gubernaculum length is 57 (50-66) mu m in the first generation males. A tail mucron is absent in first but present in second generation males. First generation males have a lateral field with a single ridge. A post-anal swelling is not developed in first generation females but is well developed in second generation females. Three other isolates of the same species were recovered from the neighbourhood of the type locality and yet another isolate was collected from Swarketari, Syangja district

Steinernema jeffreyensen. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa

“…The IJ of S. jeffreyense n. sp. has a mean body length of 926 μm, which is shorter than that of molecularly closely related species in Clade V (Spiridonov et al , 2004), except for Steinernema lamjungense Khatri-Chhetri, Waeyenberge, Spiridonov, Manadhar & Moens, 2011, with an average length of 832 (690–950) μm (table 4). Phylogenetic analysis confirmed the close relationship of S. jeffreyense n. sp.…”

“…The excretory pore of the IJ of S. jeffreyense n. sp. is more posteriorly located than is that of S. lamjungense , being 87 (78–107) μm vs. 68 (61–73) μm, respectively (Khatri-Chhetri et al , 2011), with differences in the values of D % being 66 (57–85) vs. 54 (48–63), and in those of E %, being 109 (86–169) vs. 79 (69–97) (table 4). Males of the new species differ from those of S. lamjungense , in terms of the values of D % 47 (34–68) vs. 61 (44–91), and with the SW% being 215 (171–295) vs. 173 (151–193) (table 5).…”

Steinernema innovationin. sp. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode species from South Africa

“…in clade V (as depicted by Spiridinov et al , 2004) which encompasses taxa of the ‘ glaseri -group’. Within this clade, the new species is closely related to the four described Steinernema species: S. khoisanae Nguyen et al , 2006, from South Africa and three Asian species: S. longicaudum Shen & Wang, 1992, S. lamjungense Khatri-Chhetri et al , 2011 and S. guangdongense Qiu et al , 2004. However, the novel species can also be distinguished from these taxa by several morphological and morphometric characters, which are listed below.…”

Steinernema shorin. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from India

“…2016). Steinernema nepalense Khatri-Chhetri, Waeyenberge, Spiridonov, Manandhar and Moens, 2011 and S. scapterisci Nguyen and Smart 1990 were used as outgroup taxa. The Minimum Evolution tree was searched using the Close-Neighbour-Interchange (CNI) algorithm (Nei & Kumar 2000).…”