Steel slag amendment impacts on soil microbial communities and activities of rice (Oryza sativa L.)

Abstract

With the increase in iron/steel production, the higher volume of by-products (slag) generated necessitates its efficient recycling. Because the Linz-Donawitz (LD) slag is rich in silicon (Si) and other fertilizer components, we aim to evaluate the impact of the LD slag amendment on soil quality (by measuring soil physicochemical and biological properties), plant nutrient uptake, and strengthens correlations between nutrient uptake and soil bacterial communities. We used 16 S rRNA illumine sequencing to study soil bacterial community and APIZYM assay to study soil enzymes involved in C, N, and P cycling. The LD slag was applied at 2 Mg ha −1 to Japonica and indica rice cultivated under flooded conditions. The LD slag amendment significantly improved soil pH, plant photosynthesis, soil nutrient availability, and the crop yield, irrespective of cultivars. It significantly increased N, P, and Si uptake of rice straw. The slag amendment enhanced soil microbial biomass, soil enzyme activities and enriched certain bacterial taxa featuring copiotrophic lifestyles and having the potential role for ecosystem services provided to the benefit of the plant. The study evidenced that the short-term LD slag amendment in rice cropping systems is useful to improve soil physicochemical and biological status, and the crop yield. Soil enzyme activities.Labile carbon degrading enzymes such as α-glucosidase, β-glucosidase, α-galactosidase, β-galactosidase, α-mannosidase, and α-fucosidase which can degrade maltose, cellobiose, melibiose, lactose, mannose, and fructose, respectively, markedly increased in the slag amendment treatments in comparison to the unamended treatments, irrespective of rice cultivars (Fig. 1). Likewise, recalcitrant carbon degrading enzymes such as esterase, lipase, and N-acetyl-β-glucosaminidase which can degrade hemicelluloses and polysaccharide respectively, increased by the slag amendment. Notably, the increase was more in labile carbon degrading enzymes (mostly β-glucosidase, and β-galactosidase) compared to the recalcitrant carbon degrading enzymes. The LD slag amendment also noticeably increased soil enzyme activities involved in nitrogen cycling (i.e., leucine-aminopeptidase and trypsin) and phosphorus cycling (i.e., alkaline phosphomonoesterase and phosphohydrolase). Notably, the increase in soil enzyme activities was more prominent in Indica in comparison to Japonica rice variety. Among the C cycling enzyme, β-glucosidase, and β-galactosidase were dominant, whereas among the N cycling enzyme, aminopeptidase and among the P cycling enzyme, alkaline phosphomonoesterase were dominant in the paddy soil (Fig. 1).Relative abundance and diversity of rhizosphere bacterial community. The most abundant (≥ 1.0%) bacterial phyla across treatments were Proteobacteria (34-48%), Fermicutes (15-17%), Actinobacteria (7-18%), Acidobacteria (3-7%), Bacteroidetes (2-6%), Nitrospirae (2-3%), and Chloroflexi (1-4%). The LD slag amendment significantly (P < 0.05) increased the relative abundance of Proteobacteria by 28.9 and 2...

Tres fuentes de silicio en la producción de biomasa del arroz (Oryza sativa L.) bajo condiciones controladas

“…This could be explained because of the detrimental soil pH conditions (Table 4) for those two treatments which made it impossible the normal growth of plants. Contrary to this, Das et al (2020) found that slag treatments improved soil fertility as it enhanced microbiota activity, however dozes tested in that research work were smaller. According to Yoshida (1981) and Heros (2012), Nitrogen is the main element playing a role during the tillering stage, and all experimental units got the same Nitrogen fertilization.…”

Effect of Steel Slag on Soil Fertility and Plant Growth

“…However, a positive effect on N-circulation activity was observed. This effect may be caused by the activation of ammonia and nitrite oxidizing bacteria in soil [24]. The effective addition of steel slag should consider the balance between P-circulation and N-circulation activities.…”

Individual and mixed effects of slag and biochar on soil aggregates and their carbon concentration and on fungal community structure in subtropical early‐ and late‐cropping paddy fields

“…The slag has a strong adsorption capacity, and small slag particles have large specific surface areas and large areas of contact with water, which further enhances adsorption (Piatak et al, 2015) to prevent the dispersion of clay and the destruction of aggregates (Enders et al, 2012;Jien & Wang, 2013). Each treatment significantly increased the soil carbon concentrations in the 0-15 cm layer in the paddy fields (Table 2), perhaps because the high carbon concentrations of the biochar and slag increased the soil carbon concentration (Das et al, 2020;Enders et al, 2012). Carbon was mainly focused on microaggregates (Figure 2a-d).…”