Stearoyl-CoA desaturases sustain cholinergic excitation and copulatory robustness in metabolically aging C. elegansmales

Goncalves, Jimmy; Wan, Yufeng; García, L. René

doi:10.1016/j.isci.2022.104082

Cited by 2 publications

(4 citation statements)

References 108 publications

(186 reference statements)

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“…ELO facilitates the elongation of saturated and unsaturated fatty acids [ 33 ]. DGAT and SCD catalyze the rate-limiting step of monounsaturated fatty acid synthesis [ 34 ] and triacylglycerol synthesis [ 35 ], respectively. As shown in Figure 7 A, the expression of key genes in fatty acid synthesis, elo-2 , elo-6 , fat-5 , fat-6 and fat-7 , was significantly down-regulated in C. elegans treated with astaxanthin.…”

Section: Resultsmentioning

confidence: 99%

Astaxanthin Induces Transcriptomic Responses Associated with Lifespan Extension in Caenorhabditis elegans

Ding

Zhao

2022

Antioxidants

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Astaxanthin is a marine xanthophyll carotenoid which effectively prevents intracellular oxidative stress and has beneficial effects against various human diseases. It has been shown that astaxanthin protects Caenorhabditis elegans (C. elegans) from oxidative damages and extends the lifespan of C. elegans possibly by modulating genes involved in insulin/insulin-like growth factor (IGF) signaling (IIS) and the oxidoreductase system, although the exact mechanisms remain elusive. In this study, RNA sequencing analyses were employed to identify the differentially expressed genes in C. elegans in response to astaxanthin treatment. A total of 190 mRNAs and 6 microRNAs (miRNAs) were significantly changed by astaxanthin treatment in C. elegans. Gene ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that the mRNAs and miRNAs significantly altered by astaxanthin mainly function in innate immunity, lipid metabolism and stress responses, a significant portion of which are related to lifespan regulation in C. elegans. The study revealed novel mRNA and miRNA targets of astaxanthin, providing new insights for understanding the anti-aging mechanisms and the biological function of astaxanthin.

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Section: Resultsmentioning

confidence: 99%

Astaxanthin Induces Transcriptomic Responses Associated with Lifespan Extension in Caenorhabditis elegans

Ding

Zhao

2022

Antioxidants

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“…Considering prior studies which have indicated a connection between nutrient status and behavioral prioritization, abnormal macronutrient storage or nutrient signaling of ogt-1 mutants may shift their behavior towards food seeking, resulting in less engagement in mating. Similar to the ogt-1 foodleaving phenotype, males starved overnight were less likely to leave food in the food-leaving assay [3,65], and the lipid-depleted fat-6(lf);fat-7(lf) males exhibited a behavioral preference for feeding over mating [61]. Unlike our phenotype, starving wild-type males and young adult fat-6(lf);fat-7(lf) males were fertile [61,66], suggesting the ogt-1 fertility phenotype is more complex than starvation alone.…”

Section: Plos Geneticsmentioning

confidence: 94%

“…Similar to the ogt-1 foodleaving phenotype, males starved overnight were less likely to leave food in the food-leaving assay [3,65], and the lipid-depleted fat-6(lf);fat-7(lf) males exhibited a behavioral preference for feeding over mating [61]. Unlike our phenotype, starving wild-type males and young adult fat-6(lf);fat-7(lf) males were fertile [61,66], suggesting the ogt-1 fertility phenotype is more complex than starvation alone. Future studies could assess the interactions of ogt-1 and nutrient sensing pathways such as insulin/IGF signaling, AMPK, and mTOR in the context of C. elegans male fertility.…”

Section: Plos Geneticsmentioning

confidence: 94%

“…A recent study compared the gene expression profiles of adult C. elegans tissues with human tissues and interestingly found the C. elegans hypodermis to be most similar to the human liver, a correlation driven by the hypodermal expression of metabolic enzymes and regulators [60]. The metabolic functions of the hypodermis have previously been shown to play key roles in male mating by storing and mobilizing energy to fuel neurons and muscles directly involved in mating [61,62]. Highlighting the connection between the hypodermis and behavioral prioritization, the hypodermal rescue line showed comparable food-leaving behavior to wild-type (Fig 1B) in addition to rescuing the brood size.…”

Section: Plos Geneticsmentioning

confidence: 99%

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O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility

et al. 2022

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Animal behavior is influenced by the competing drives to maintain energy and to reproduce. The balance between these evolutionary pressures and how nutrient signaling pathways intersect with mating remains unclear. The nutrient sensor O-GlcNAc transferase, which post-translationally modifies intracellular proteins with a single monosaccharide, is responsive to cellular nutrient status and regulates diverse biological processes. Though essential in most metazoans, O-GlcNAc transferase (ogt-1) is dispensable in Caenorhabditis elegans, allowing genetic analysis of its physiological roles. Compared to control, ogt-1 males had a four-fold reduction in mean offspring, with nearly two thirds producing zero progeny. Interestingly, we found that ogt-1 males transferred sperm less often, and virgin males had reduced sperm count. ogt-1 males were also less likely to engage in mate-searching and mate-response behaviors. Surprisingly, we found normal fertility for males with hypodermal expression of ogt-1 and for ogt-1 strains with catalytic-dead mutations. This suggests OGT-1 serves a non-catalytic function in the hypodermis impacting male fertility and mating behavior. This study builds upon research on the nutrient sensor O-GlcNAc transferase and demonstrates a role it plays in the interplay between the evolutionary drives for reproduction and survival.

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Stearoyl-CoA desaturases sustain cholinergic excitation and copulatory robustness in metabolically aging C. elegansmales

Cited by 2 publications

References 108 publications

Astaxanthin Induces Transcriptomic Responses Associated with Lifespan Extension in Caenorhabditis elegans

Astaxanthin Induces Transcriptomic Responses Associated with Lifespan Extension in Caenorhabditis elegans

O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility

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