Steam volatile amines of Latakia tobacco leaf

Irvine, W. J.; Saxby, M.J.

doi:10.1016/s0031-9422(00)85450-3

Cited by 31 publications

(8 citation statements)

References 5 publications

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“…In an intensive study of the steam-volatile amines in cured tobacco leaves, a large range of primary, secondary and tertiary amines has been demonstrated (Neurath, Ehmke & Miiller, 1964;Yasumatsu & Akaike, 1965;Neurath, Krull, Pirmann & Wandrey, 1966;Irvine & Saxby, 1969;Bush, Sims & Atkinson, 1970). Aliphatic amines have also been found in the seed and seedlings of Viau faba L. and Zea mays L. (Preusser, 1966) and in the tubers of Solanum tuberosum L. (Schormiiller & Weder, I 966).…”

Section: T a Smithmentioning

confidence: 99%

The Occurrence, Metabolism and Functions of Amines in Plants

Smith

1971

Biological Reviews

140

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Section: T a Smithmentioning

confidence: 99%

The Occurrence, Metabolism and Functions of Amines in Plants

Smith

1971

Biological Reviews

140

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“…The pipecolic acid, tyramine, phenethylamine, isoamylamine, and homospermidine were tentatively identified by high‐accuracy protonated molecules [M+H] + at m/z 300.1443, 308.1496, 292.1544, 258.1711, 670.3546 with mass error of 1.33, 0.65, 1.71, 2.32, 0.75 ppm (Supporting Information Fig. S1–S5 and ).…”

Section: Resultsmentioning

confidence: 99%

Free amino acids, biogenic amines, and ammonium profiling in tobacco from different geographical origins using microwave‐assisted extraction followed by ultra high performance liquid chromatography

Cai¹,

Xiang²,

et al. 2017

J of Separation Science

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This work describes a rapid, stable, and accurate method for determining the free amino acids, biogenic amines, and ammonium in tobacco. The target analytes were extracted with microwave-assisted extraction and then derivatized with diethyl ethoxymethylenemalonate, followed by ultra high performance liquid chromatography analysis. The experimental design used to optimize the microwave-assisted extraction conditions showed that the optimal extraction time was 10 min with a temperature of 60°C. The stability of aminoenone derivatives was improved by keeping the pH near 9.0, and there was no obvious degradation during the 80°C heating and room temperature storage. Under optimal conditions, this method showed good linearity (R > 0.999) and sensitivity (limits of detection 0.010-0.081 μg/mL). The extraction recoveries were between 88.4 and 106.5%, while the repeatability and reproducibility ranged from 0.48 to 5.12% and from 1.56 to 6.52%, respectively. The newly developed method was employed to analyze the tobacco from different geographical origins. Principal component analysis showed that four geographical origins of tobacco could be clearly distinguished and that each had their characteristic components. The proposed method also showed great potential for further investigations on nitrogen metabolism in plants.

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“…The aliphatic amines present in tobacco and its smoke [1][2][3][4] are supposed to originate from a breakdown of proteinaceous material and/or amino acids during processing as a result of microbial or enzymatic action or certain circumstances when relatively high temperatures are encountered [5]. Nicotine and other alkaloids are also the sources of aliphatic amines [6].…”

Section: Introductionmentioning

confidence: 99%

Development of single‐drop microextraction and simultaneous derivatization followed by GC‐MS for the determination of aliphatic amines in tobacco

Sha

Meng

Lin

et al. 2010

J of Separation Science

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In this work, for the first time, headspace (HS) single-drop microextraction and simultaneous derivatization followed by GC-MS was developed to determine the aliphatic amines in tobacco samples. In the HS extraction procedure, the mixture of derivatization reagent and organic solvent was employed as the extraction solvent for HS single-drop microextraction and in situ derivatization of aliphatic amine in the samples. Fast extraction and simultaneous derivatization of the analytes were performed in a single step, and the obtained derivatives in the microdrop extraction solvent were analyzed by GC-MS. The optimized experiment conditions were: sample preparation temperature of 80 degrees C and time of 30 min, HS extraction solvent (the mixture of benzyl alcohol and 2,3,4,5,6-pentafluorobenzaldehyde) volume of 2.0 microL, extraction time of 90 s. With the optimal conditions, the method validations were also studied. The method has good linearity (R(2) more than 0.99), accepted precision (RSD less than 13%), good recovery (98-104%) and low limit of detection (0.11-0.97 microg/g). Finally, the proposed technique was successfully applied to the analyses of aliphatic amines in tobacco samples of seven different brands. It was further demonstrated that the proposed method offered a simple, low-cost and reliable approach to determine aliphatic amines in tobacco samples.

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Steam volatile amines of Latakia tobacco leaf

Cited by 31 publications

References 5 publications

The Occurrence, Metabolism and Functions of Amines in Plants

The Occurrence, Metabolism and Functions of Amines in Plants

Free amino acids, biogenic amines, and ammonium profiling in tobacco from different geographical origins using microwave‐assisted extraction followed by ultra high performance liquid chromatography

Development of single‐drop microextraction and simultaneous derivatization followed by GC‐MS for the determination of aliphatic amines in tobacco

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