Steady state peripheral blood provides cells with functional and metabolic characteristics of real hematopoietic stem cells

Bourdieu, Antonin; Avalon, Maryse; Lapostolle, Veronique; Ismail, Sadek; Mombled, Margaux; Debeissat, Christelle; Guérinet, Marianne; Duchez, Pascale; Chevaleyre, J.; Vlaski‐Lafarge, Marija; Villacreces, Arnaud; Perreten, Vincent; Ivanović, Zoran; Grange, Philippe Brunet de la

doi:10.1002/jcp.25881

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…Murine studies demonstrated HSC migration to ectopic niches via the circulation (Christensen et al, 2004;Wright et al, 2001). HSCs were detected in the blood of 12-to 18-week-old human fetuses (Gallacher et al, 2000), and long-term repopulating HSCs were demonstrated in adult human steady-state peripheral blood (Bourdieu et al, 2017;Brunet de la Grange et al, 2013). We now demonstrate the turnover of ''ectopic'' niche HSPCs from the circulating pool in humans.…”

Section: (Legend Continued On Next Page)mentioning

confidence: 59%

Human Intestinal Allografts Contain Functional Hematopoietic Stem and Progenitor Cells that Are Maintained by a Circulating Pool

Martínez

Shonts

et al. 2019

Cell Stem Cell

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Section: (Legend Continued On Next Page)mentioning

confidence: 59%

Human Intestinal Allografts Contain Functional Hematopoietic Stem and Progenitor Cells that Are Maintained by a Circulating Pool

Martínez

Shonts

et al. 2019

Cell Stem Cell

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“…In humans, the SP phenotype has been discovered in HSCs (hHSCs) isolated from the peripheral blood [34, 59], umbilical cord blood [35], and bone marrow [13].…”

Section: Side Population and Stem Cellsmentioning

confidence: 99%

“…The SP cells in hHSCs derived from peripheral blood were also reported to be CD34 + and CD38 − . These SP cells displayed greater proliferative capacity and generated more clonogenic progenitors during culturing and also showed greater engraftment capabilities in xenotransplantation studies [34, 59].…”

Section: Side Population and Stem Cellsmentioning

confidence: 99%

Side Population: Its Use in the Study of Cellular Heterogeneity and as a Potential Enrichment Tool for Rare Cell Populations

Wolmarans

Nel

Durandt

et al. 2018

Stem Cells International

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There is still much to learn about the cells used for cell- and gene-based therapies in the clinical setting. Stem cells are found in virtually all tissues in the human body. As a result, cells isolated from these tissues are a heterogeneous population consisting of various subpopulations including stem cells. Several strategies have been used to isolate and define the subpopulations that constitute these heterogeneous populations, one of which is the side population (SP) assay. SP cells are identified by their ability to efflux a fluorescent dye at a rate that is greater than the main cell population. This elevated rate of dye efflux has been attributed to the expression of members of the ATP-binding cassette (ABC) transporter protein family. SP cells have been identified in various tissues. In this review, we discuss the research to date on SP cells, focussing on SP cells identified in haematopoietic stem cells, adipose-derived stromal cells, and dental pulp.

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“…Nonetheless, despite the clinical efficiency of mPB in HCT and the global good tolerance of the mobilization regimen for the donor, PB mobilization can induce severe adverse effects in rare cases and nearly all donors experience inconveniences such as bone pain, headache, and nausea [5]. Therefore, as SSPB HSCs possess functional characteristics of real HSCs [6][7][8][9][10], they represent an easy and interesting source of HSCs for HCT, provided reliable ex vivo procedures to amplify HSC to numbers sufficient for an effective engraftment are developed. The ex vivo expansion of HSC without the loss of a marrow repopulating ability and long-term stem cell potential is a challenging question.…”

Section: Introductionmentioning

confidence: 99%

Alpha Lipoic-Acid Potentiates Ex Vivo Expansion of Human Steady-State Peripheral Blood Hematopoietic Primitive Cells

et al. 2022

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Steady state peripheral blood (SSPB) contains hematopoietic stem and progenitor cells (HSPCs) presenting characteristics of real hematopoietic stem cells, and thus represents an interesting alternative cell supply for hematopoietic cell transplantation. Development of ex vivo expansion strategies could overcome the low HSPC numbers usually rescued from SSPB. We investigated the effect of alpha lipoic acid (ALA) on ex vivo culture of SSPB CD34 positive (CD34pos) cells on primitive cell expansion, cell cycle, and oxidative metabolism as estimated by determining the ROS and GSH content. ALA increased the ex vivo expansion of total CD34pos cells and of phenotypically defined CD34pos HSPCs subpopulations that retained in vivo repopulating capacity, concomitantly to a decreased expansion of differentiating cells. ALA did not modify cell cycle progression nor the proliferation of ex vivo expanded CD34pos cells, and coherently did not affect the ROS level. On the contrary, ALA decreased the proliferation and disturbed cell cycle progression of cells reaching a differentiated status, a phenomenon that seems to be associated with a drop in ROS level. Nonetheless, ALA affected the redox status of hematopoietic primitive cells, as it reproducibly increased GSH content. In conclusion, ALA represents an interesting molecule for the improvement of ex vivo expansion strategies and further clinical application in hematopoietic cell transplantation (HCT).

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Steady state peripheral blood provides cells with functional and metabolic characteristics of real hematopoietic stem cells

Abstract: Hematopoietic stem cells (HSCs), which are located in the bone marrow, also circulate in cord and peripheral blood. Despite high availability, HSCs from steady state peripheral blood (SSPB) are little known and not used for research or cell therapy. We thus aimed to characterize and

Cited by 10 publications

References 33 publications

Human Intestinal Allografts Contain Functional Hematopoietic Stem and Progenitor Cells that Are Maintained by a Circulating Pool

Human Intestinal Allografts Contain Functional Hematopoietic Stem and Progenitor Cells that Are Maintained by a Circulating Pool

Side Population: Its Use in the Study of Cellular Heterogeneity and as a Potential Enrichment Tool for Rare Cell Populations

Alpha Lipoic-Acid Potentiates Ex Vivo Expansion of Human Steady-State Peripheral Blood Hematopoietic Primitive Cells

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