Steady-State Free Precession sequences for high and low field NMR spectroscopy in solution: Challenges and opportunities

Moraes, Tiago Bueno; Kock, Flávio Vinícius Crizóstomo; Salomé, Kahlil Schwanka; Barison, Andersson; Simpson, André J.; Colnago, Luiz Alberto

doi:10.1016/j.jmro.2022.100090

Cited by 1 publication

(3 citation statements)

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“…In order to improve the signal-to-noise ratio, the steady state free precession (SSFP) sequence [ 52 , 70 ] was used. This is a technique that has recently received renewed attention [ 52 , 71 ] and works by employing very short pulse-acquisition blocks, allowing for a much larger number of transients to be acquired. This can result in significant signal-to-noise improvements.…”

Section: Methodsmentioning

confidence: 99%

“…The standard spectrum was acquired using 10,240 scans, 32,768 time domain points and a recycle delay of 3 s, and compared to an SSFP spectrum For 13 C experiments, a single 13 C labelled broccoli seed (Brassica oleracea, IsoLife) was placed in water for one week in order to induce sprouting and was then placed in a thin-walled capillary and analyzed on the micro-coil system. In order to improve the signal-to-noise ratio, the steady state free precession (SSFP) sequence [52,70] was used. This is a technique that has recently received renewed attention [52,71] and works by employing very short pulse-acquisition blocks, allowing for a much larger number of transients to be acquired.…”

Section: Methodsmentioning

confidence: 99%

“…For 1D NMR this is commonly set to 3-5 times the T1 of the sample being studied, which can be 5-10 s or longer for 13 C experiments. On the other hand, SSFP works by pulsing faster (often with a small flip angle pulse) than the relaxation time of the sample, which results in a steady state of magnetization that can be measured [52]. In practice, the time between successive pulses can be on the order of 10s of milliseconds 13 C spectrum of a 13 C labelled broccoli seed acquired at 11.7 T (500 MHz 1 H).…”

Section: Ssfp Compared To Standard 13 C Nmrmentioning

confidence: 99%

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Exploring the Potential of Broadband Complementary Metal Oxide Semiconductor Micro-Coil Nuclear Magnetic Resonance for Environmental Research

Lysak,

Grisi,

Marable

et al. 2023

Molecules

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With sensitivity being the Achilles’ heel of nuclear magnetic resonance (NMR), the superior mass sensitivity offered by micro-coils can be an excellent choice for tiny, mass limited samples such as eggs and small organisms. Recently, complementary metal oxide semiconductor (CMOS)-based micro-coil transceivers have been reported and demonstrate excellent mass sensitivity. However, the ability of broadband CMOS micro-coils to study heteronuclei has yet to be investigated, and here their potential is explored within the lens of environmental research. Eleven nuclei including 7Li, 19F, 31P and, 205Tl were studied and detection limits in the low to mid picomole range were found for an extended experiment. Further, two environmentally relevant samples (a sprouting broccoli seed and a D. magna egg) were successfully studied using the CMOS micro-coil system. 13C NMR was used to help resolve broad signals in the 1H spectrum of the 13C enriched broccoli seed, and steady state free precession was used to improve the signal-to-noise ratio by a factor of six. 19F NMR was used to track fluorinated contaminants in a single D. magna egg, showing potential for studying egg–pollutant interactions. Overall, CMOS micro-coil NMR demonstrates significant promise in environmental research, especially when the future potential to scale to multiple coil arrays (greatly improving throughput) is considered.

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