StCaM2, a calcium binding protein, alleviates negative effects of salinity and drought stress in tobacco

Raina, Meenakshi; Kumar, Ashish; Yadav, Nikita; Kumari, Sumita; Yusuf, Mohammad; Mustafiz, Ananda; Kumar, Deepak

doi:10.1007/s11103-021-01131-1

Cited by 25 publications

(8 citation statements)

References 75 publications

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“…MDA, H 2 O 2 and ion leakage are also considered an indicator of oxidative damage and membrane injury (Hasegawa et al 2000). Following prior reports, our ndings also indicate that the EcCaM overexpression lines contain lesser MDA and show lesser ion leakage as reported for StCaM2 (Raina et al 2021) and another Ca 2+ sensor MsCBL4 (An et al 2020). We posit that overexpression of EcCaM leads to elevated proline associated osmotic potential balance under stress and lesser membrane damage and ion leakage thereby resulting in the observed tolerant phenotype.…”

Section: Discussionsupporting

confidence: 86%

Ectopic expression of finger millet calmodulin confers drought and salinity tolerance in Arabidopsis thaliana

et al. 2021

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Drought and salinity are major environmental stresses which affect crop productivity and therefore are major hindrance in feeding growing population world-wide. Calcium (Ca 2+ ) signalling plays a crucial role during the plant's response to these stress stimuli. Calmodulin (CaM), a crucial Ca 2+ sensor, is involved in transducing the signal downstream in various physiological, developmental and stress responses by modulating a plethora of target proteins. The role of CaM has been well established in the model plant Arabidopsis thaliana for regulating various developmental processes, stress signalling and ion transport. In the current study, we investigate the CaM of Eleusine coracana (common name nger millet, known especially for its drought tolerance and superior Ca 2+ content). In-silico analysis showed that Eleucine calmodulin (EcCaM) has greater similarity to rice CaM as compared to Arabidopsis CaM due to the presence of highly conserved four EF-hand domains. To decipher the in planta function of EcCaM, we have adopted the gain-of-function approach by generating the 35S::EcCaM over-expression transgenic in Arabidopsis. Overexpression of EcCaM in Arabidopsis makes the plant tolerant to polyethylene glycol (PEG) induced drought and salt stress (NaCl) as demonstrated by post-germination based phenotypic assay, ion leakage, MDA and proline estimation, ROS detection under stressed and normal conditions. Moreover, EcCaM-overexpression leads to hypersensitivity towards exogenously applied ABA at the seed germination stage. These ndings reveal that EcCaM mediates tolerance to drought and salinity stress. Also, our results indicate that EcCaM is involved in modulating ABA signalling. Summarizing our results, we report for the rst time that EcCaM is involved in modulating plants response to stress and this information can be used for the generation of future-ready crops that can tolerate a wide range of abiotic stresses.

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Section: Discussionsupporting

confidence: 86%

Ectopic expression of finger millet calmodulin confers drought and salinity tolerance in Arabidopsis thaliana

et al. 2021

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“…Accumulation of ROS during abiotic stress leads to the accumulation of MDA and damages to cellular membranes, making them leaky, which ultimately results in the outflow of ions that are vital for proper cell functioning (Garg et al, 2017; Raina et al, 2021). We observed lower amounts of MDA and ion leakage in the CB lines than in γ‐TMT , gly I , and WT lines under stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Pyramiding of γ‐TMT and gly I transgenes in Brassica juncea enhances salinity and drought stress tolerance

Kumar

Rajwanshi

Singh

et al. 2022

Physiologia Plantarum

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We previously generated Brassica juncea lines overexpressing either glyoxalase I (gly I) or γ-tocopherol methyltransferase (γ-TMT) involved in the glyoxalase system and tocopherol biosynthesis, respectively. These transgenic plants showed tolerance to multiple abiotic stresses. As tolerance is a complex trait that can be improved by pyramiding of several characteristics in a single genotype, we generated in this study B. juncea plants coexpressing gly I and γ-TMT by crossing the previously generated stable transgenic lines. The performance of the newly generated B. juncea lines coexpressing gly I and γ-TMT was compared with that of wild-type and the single transgenic lines under non-stressed and NaCl and mannitol stress conditions. Our results show a more robust antioxidant response of B. juncea plants coexpressing gly I and γ-TMT compared to the other lines in terms of higher chlorophyll retention, relative water content, antioxidant enzyme and proline levels, and photosynthetic efficiency and lower oxidative damage. The differences in response to the stress of the different lines were reflected in their yield parameters. Overall, we demonstrate that the pyramiding of multiple genes involved in antioxidant pathways could be a viable and useful approach for achieving higher abiotic stress tolerance in crop plants.

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“…Likewise, the application of ethephon and prohexadione-Ca 2+ significantly affects the vegetative growth, reproductive phase and productivity of young 'Northern Spy' and 'Jonagold' apple trees (Duyvelshoff & Cline, 2013). Furthermore, a recent study on transgenic tobacco plants grown under drought stress revealed that the Ca 2+ binding protein, calmodulin (StCaM2), mitigates the detrimental effects of drought stress by regulating osmolytes accumulation, enhancing cell membrane stability and reducing ROS concentration, thereby helping the plants maintain osmotic equilibrium and improve photosynthetic performance (Raina et al, 2021).…”

Section: Crosstalk Of Ethylene and Other Plant Signaling Moleculesmentioning

confidence: 99%

“…Among a variety of secondary signaling molecules, calcium (Ca 2+ ) ion is one of the primary participants in environmental stress responses, mediating numerous physiological processes (Yang et al, 2021c). There are indications that ethylene and Ca 2+ ‐dependent pathways are associated with drought stress mechanisms (Zhang et al, 2018b; Raina et al, 2021). For instance, seed priming with ethephon enhances the water stress tolerance of two kentucky bluegrass cultivars by maintaining plasma membrane permeability and elevating the levels of proline, soluble proteins, as well as root Ca 2+ and potassium (K + ) (Zhang et al, 2018b; Table 2).…”

Section: Crosstalk Of Ethylene With Other Plant Growth Regulators Und...mentioning

confidence: 99%

Plant hormone ethylene: A leading edge in conferring drought stress tolerance

Nazir,

Peter,

Gupta

et al. 2024

Physiologia Plantarum

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Agricultural sufficient productivity is of paramount importance for ensuring food security and conserving soil health to support the world's agronomy. Climatic abruptions have been emerging as one of the most nerve‐pressing issues for the sustainment of the planet Earth in the twenty‐first century. Among the various environmental constraints, drought stress stands out as a potent factor restricting crop growth and productivity. It triggers a myriad of intricate responses in plants to combat the underlying stress‐mediated adversities. Gaining a comprehensive understanding of the key physiological and molecular mechanisms that enable plants to withstand drought stress is crucial for developing effective strategies to enhance crop resilience. Ethylene, a gaseous plant hormone, influences the adaptive measures adopted by plants subjected to drought stress by regulating the drought stress‐mediated signal transduction‐associated responses. The present review article provides an in‐depth understanding of the critical roles of ethylene in enhancing plants' ability to restrain the severity of drought stress. It also highlights the significance of ethylene signaling components in regulating plant survival and drought stress tolerance. Additionally, we have illustrated the additive and antagonistic interactions of ethylene with other plant growth regulators, which instigate the tolerance responses. Conclusively, this review emphasizes the significance of complex networks involved in ethylene‐mediated drought tolerance, providing valuable insights for future research and uncovering novel studies in the field of ethylene biology.

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StCaM2, a calcium binding protein, alleviates negative effects of salinity and drought stress in tobacco

Cited by 25 publications

References 75 publications

Ectopic expression of finger millet calmodulin confers drought and salinity tolerance in Arabidopsis thaliana

Ectopic expression of finger millet calmodulin confers drought and salinity tolerance in Arabidopsis thaliana

Pyramiding of γ‐TMT and gly I transgenes in Brassica juncea enhances salinity and drought stress tolerance

Plant hormone ethylene: A leading edge in conferring drought stress tolerance

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