Stbd1-deficient mice display insulin resistance associated with enhanced hepatic ER-mitochondria contact

Kyriakoudi, Styliana; Theodoulou, Andria; Potamiti, Louiza; Schumacher, Fabian; Zachariou, Margarita; Papacharalambous, Revekka; Kleuser, Burkhard; Panayiotidis, Mihalis Ι.; Drousiotou, Anthi; Petrou, Petros

doi:10.1016/j.biochi.2022.06.003

Cited by 4 publications

(6 citation statements)

References 35 publications

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“…ERS could lead to the accumulation of unfolded proteins, contributing to myocardial apoptosis in diabetic animal models (Liu et al, 2021;Wu, Lu, et al, 2023;. (Kyriakoudi et al, 2022;Lytridou et al, 2020). Moreover, STBD1 is considered a selective autophagic receptor for glycogen and an important glycophagy medium.…”

Section: Discussionmentioning

confidence: 99%

“…STBD1 is a glycogen‐binding protein resident in the endoplasmic reticulum. STBD1 was identified as the downstream target of ERS response in C2C12 cells, specifically promoting the formation of endoplasmic reticulum‐related glycogen clusters and responding to ERS activation (Kyriakoudi et al, 2022; Lytridou et al, 2020). Moreover, STBD1 is considered a selective autophagic receptor for glycogen and an important glycophagy medium.…”

Section: Discussionmentioning

confidence: 99%

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D‐pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin‐mediated endoplasmic reticulum stress and glycophagy signaling pathway

Li,

Yu,

et al. 2024

Phytotherapy Research

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Diabetic cardiomyopathy (DCM) is an important complication resulting in heart failure and death of diabetic patients. However, there is no effective drug for treatments. This study investigated the effect of D‐pinitol (DP) on cardiac injury using diabetic mice and glycosylation injury of cardiomyocytes and its molecular mechanisms. We established the streptozotocin‐induced SAMR1 and SAMP8 mice and DP (150 mg/kg/day) intragastrically and advanced glycation end‐products (AGEs)‐induced H9C2 cells. H9C2 cells were transfected with optineurin (OPTN) siRNA and overexpression plasmids. The metabolic disorder indices, cardiac dysfunction, histopathology, immunofluorescence, western blot, and immunoprecipitation were investigated. Our results showed that DP reduced the blood glucose and AGEs, and increased the expression of heart OPTN in diabetic mice and H9C2 cells, thereby inhibiting the endoplasmic reticulum stress (GRP78, CHOP) and glycophagy (STBD1, GABARAPL1), and alleviating the myocardial apoptosis and fibrosis of DCM. The expression of filamin A as an interaction protein of OPTN downregulated by AGEs decreased OPTN abundance. Moreover, OPTN siRNA increased the expression of GRP78, CHOP, STBD1, and GABARAPL1 and inhibited the expression of GAA via GSK3β phosphorylation and FoxO1. DP may be helpful to treat the onset of DCM. Targeting OPTN with DP could be translated into clinical application in the fighting against DCM.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

D‐pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin‐mediated endoplasmic reticulum stress and glycophagy signaling pathway

Li,

Yu,

et al. 2024

Phytotherapy Research

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“…Thus, STBD1 may act as a novel resident protein for other subcellular organelles with untapped biological functions. Notably, STBD1 is also localized to the MAM, playing a novel role in glucose homeostasis (Kyriakoudi et al, 2022). STBD1 knockout increased ER‐mitochondria association and enhanced mitochondrial fragmentation in the liver.…”

Section: Discussionmentioning

confidence: 99%

“…STBD1 knockout mice showed a variety of insulin-resistant metabolic abnormalities, such as significantly reduced liver glycogen content during fasting, abnormally increased blood glucose levels after fasting and refeeding, reduced response to insulin, and enhanced gluconeogenesis. Overall, STBD1 maintains hepatic MAM integrity and plays a new role in controlling glucose metabolism (Kyriakoudi et al, 2022).…”

Section: Stbd1 Deficiency Is a Susceptibility Factor For Insulin Resi...mentioning

confidence: 99%

“…STBD1 is an ER resident protein that is also located at MAM (Demetriadou et al, 2017). Styliana et al observed the mitochondrial morphology and ER‐mitochondrial contact in the liver of STBD1 knockout mice, and found that the mitochondria appeared round and ER‐mitochondrial contact increased (Kyriakoudi et al, 2022). This suggests that STBD1 can maintain the normal morphology of MAM and mitochondria, while knockout of STBD1 induces MAM distortion and mitochondrial morphological changes.…”

Section: Biological Functionsmentioning

confidence: 99%

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Glycogen‐binding protein STBD1: Molecule and role in pathophysiology

Tang

Liu

Zhao

et al. 2023

Journal Cellular Physiology

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Starch‐binding domain‐containing protein 1 (STBD1) is a glycogen‐binding protein discovered in skeletal muscle gene differential expression that is pivotal to cellular energy metabolism. Recent studies have indicated that STBD1 is involved in many physiological processes, such as glycophagy, glycogen accumulation, and lipid droplet formation. Moreover, dysregulation of STBD1 causes multiple diseases, including cardiovascular disease, metabolic disease, and even cancer. Deletions and/or mutations in STBD1 promote tumorigenesis. Therefore, STBD1 has garnered considerable interest in the pathology community. In this review, we first summarized the current understanding of STBD1, including its structure, subcellular localization, tissue distribution, and biological functions. Next, we examined the roles and molecular mechanisms of STBD1 in related diseases. Based on available research, we discussed the novel function and future of STBD1, including its potential application as a therapeutic target in glycogen‐related diseases. Given the significance of STBD1 in energy metabolism, an in‐depth understanding of the protein is crucial for understanding physiological processes and developing therapeutic strategies for related diseases.

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Hepatic ChREBP orchestrates intrahepatic carbohydrate metabolism to limit hepatic glucose 6-phosphate and glycogen accumulation in a mouse model for acute Glycogen Storage Disease type Ib

Krishnamurthy,

Rutten,

Hoogerland

et al. 2024

Molecular Metabolism

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Stbd1-deficient mice display insulin resistance associated with enhanced hepatic ER-mitochondria contact

Cited by 4 publications

References 35 publications

D‐pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin‐mediated endoplasmic reticulum stress and glycophagy signaling pathway

D‐pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin‐mediated endoplasmic reticulum stress and glycophagy signaling pathway

Glycogen‐binding protein STBD1: Molecule and role in pathophysiology

Hepatic ChREBP orchestrates intrahepatic carbohydrate metabolism to limit hepatic glucose 6-phosphate and glycogen accumulation in a mouse model for acute Glycogen Storage Disease type Ib

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