Stavudine loaded gelatin liposomes for HIV therapy: Preparation, characterization and in vitro cytotoxic evaluation

Nayak, Debasis; Boxi, Ankita; Ashe, Sarbani; Thathapudi, Neethi Chandra; Nayak, Bismita

doi:10.1016/j.msec.2016.12.073

Cited by 53 publications

(26 citation statements)

References 46 publications

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“…A number of pharmacological components were detected by UPLC–QTOF‐MS. Some of them were increased significantly ( P < 0.05) during IF (Table 4) including stavudine (Nayak, Boxi, Ashe, Thathapudi, & Nayak, ), ketoprofen, d ‐biotin, baclofen, capecitabine, rosiglitazone, and tolbutamide. The health benefits of tea are improved due to pharmacological components produced in fermentation, which includes in improving rheumatoid arthritis (Zafar et al., ), arthrolithiasis (Takara et al., ; Zafar et al., ), anticancer (Leicher, Graal, Coers, Tascilar, & Groot, ) and diabetes treatment (Drebushchak, Drebushchak, Pankrushina, & Boldyreva, ).…”

Section: Resultsmentioning

confidence: 99%

LC–MS/MS‐based metabolomic analysis of caffeine‐degrading fungus Aspergillus sydowii during tea fermentation

Zhou

Xiaoying

et al. 2020

Journal of Food Science

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Natural microorganisms involved in solid-state fermentation (SSF) of Pu-erh tea have a significant impact on its chemical components. Aspergillus sydowii is a fungus with a high caffeine-degrading capacity. In this work, A. sydowii was inoculated into sun-dried green tea leaves for SSF. Metabolomic analysis was carried out by using UPLC-QTOF-MS method, and caffeine and related demethylated products were determined by HPLC. The results showed that A. sydowii had a significant (P < 0.05) impact on amino acids, carbohydrates, flavonoids, and caffeine metabolism. Moreover, A. sydowii could promote the production of ketoprofen, baclofen, and tolbutamide. Along with caffeine degradation, theophylline, 3methylxanthine, 1,7-dimethylxanthine, 1-methylxanthine, and 7-methylxanthine were increased significantly (P < 0.05) during inoculated fermentation, which showed that demethylation was the main pathway of caffeine degradation in A. sydowii secondary metabolism. The absolute quantification analysis showed that caffeine could be demethylated and converted to theophylline and 3-methylxanthine. Particularly, about 93.24% of degraded caffeine was converted to theophylline, 27.92 mg/g of theophylline was produced after fermentation.

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Section: Resultsmentioning

confidence: 99%

LC–MS/MS‐based metabolomic analysis of caffeine‐degrading fungus Aspergillus sydowii during tea fermentation

Zhou

Xiaoying

et al. 2020

Journal of Food Science

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“…Hemagglutination activity of lyo-secretome was evaluated following the procedure reported by [ 35 , 36 ] with slight modifications. Briefly, 100 μL of the RBCs pellet was mixed with 10 mL of PBS (pH 7.4) and used in the assay.…”

Section: Methodsmentioning

confidence: 99%

Pilot Production of Mesenchymal Stem/Stromal Freeze-Dried Secretome for Cell-Free Regenerative Nanomedicine: A Validated GMP-Compliant Process

Bari

Perteghella

Silvestre

et al. 2018

Cells

118

163

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In this paper, a pilot production process for mesenchymal stem/stromal freeze-dried secretome was performed in a validated good manufacturing practice (GMP)-compliant cell factory. Secretome was purified from culture supernatants by ultrafiltration, added to cryoprotectant, lyophilized and characterized. We obtained a freeze-dried, “ready-off-the-shelf” and free soluble powder containing extracellular vesicles and proteins. In the freeze-dried product, a not-aggregated population of extracellular vesicles was detected by nanoparticle tracking analysis; Fourier transform infrared spectra showed the simultaneous presence of protein and lipids, while differential scanning calorimetry demonstrated that lyophilization process successfully occurred. A proteomic characterization allowed the identification of proteins involved in immune response, response to stress, cytoskeleton and metabolism. Moreover, the product was not cytotoxic up to concentrations of 25 mg/mL (on human fibroblasts, chondrocytes and nucleus pulposus cells by MTT assay) and was blood compatible up to 150 mg/mL. Finally, at concentrations between 5 and 50 mg/mL, freeze-dried secretome showed to in vitro counteract the oxidative stress damage induced by H2O2 on nucleus pulposus cells by MTT assay.

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“…For instance, Mandal et al [69] reported that a conventional anti-retroviral drug, emtricitabine, was encapsulated in PLGA polymeric nanoparticles to circumvent the limitations associated with the large volume of distribution, short plasma life, low bioavailability, and cytotoxic nature of the drug to improve its efficacy for the therapeutic treatment of HIV infection. Similar studies have demonstrated the encapsulation of conventional anti-retroviral drugs such as zidovudine, a combination of three agents (zidovudine, efavirenz, and lamivudine); and stavudine in PF-68-coated alginate nanoparticles, and lactoferrin nanoparticles and gelatine liposomal nano-formulations have been reported as strategies for enhanced anti-HIV therapy [70][71][72]. Efficient microbicide action was displayed by a combination of a HIV reverse transcriptase-inhibiting drug (efavirenz) and an integrase inhibitor (elvitegravir) encapsulated in a graft copolymer of methoxypolyethylene glycol-polylysine with a fatty hydrophobic core.…”

Section: Sexually Transmitted Infections and Hivmentioning

confidence: 69%

Nanotechnology in reproductive medicine: Opportunities for clinical translation

Shandilya

Pathak

Lohiya

et al. 2020

Clin Exp Reprod Med

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In recent years, nanotechnology has revolutionized global healthcare and has been predicted to exert a remarkable effect on clinical medicine. In this context, the clinical use of nanomaterials for cancer diagnosis, fertility preservation, and the management of infertility and other pathologies linked to pubertal development, menopause, sexually transmitted infections, and HIV (human immunodeficiency virus) has substantial promise to fill the existing lacunae in reproductive healthcare. Of late, a number of clinical trials involving the use of nanoparticles for the early detection of reproductive tract infections and cancers, targeted drug delivery, and cellular therapeutics have been conducted. However, most of these trials of nanoengineering are still at a nascent stage, and better synergy between pharmaceutics, chemistry, and cutting-edge molecular sciences is needed for effective translation of these interventions from bench to bedside. To bridge the gap between translational outcome and product development, strategic partnerships with the insight and ability to anticipate challenges, as well as an in-depth understanding of the molecular pathways involved, are highly essential. Such amalgamations would overcome the regulatory gauntlet and technical hurdles, thereby facilitating the effective clinical translation of these nano-based tools and technologies. The present review comprehensively focuses on emerging applications of nanotechnology, which holds enormous promise for improved therapeutics and early diagnosis of various human reproductive tract diseases and conditions.

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Stavudine loaded gelatin liposomes for HIV therapy: Preparation, characterization and in vitro cytotoxic evaluation

Cited by 53 publications

References 46 publications

LC–MS/MS‐based metabolomic analysis of caffeine‐degrading fungus Aspergillus sydowii during tea fermentation

LC–MS/MS‐based metabolomic analysis of caffeine‐degrading fungus Aspergillus sydowii during tea fermentation

Pilot Production of Mesenchymal Stem/Stromal Freeze-Dried Secretome for Cell-Free Regenerative Nanomedicine: A Validated GMP-Compliant Process

Nanotechnology in reproductive medicine: Opportunities for clinical translation

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