Status Report on the Microbial Characterization of Halite and Groundwater Samples from the WIPP

Swanson, Juliet S.; Reed, Donald T.; David, A.; Norden, Diana M.; Simmons, Karen A.

doi:10.2172/1045985

Cited by 7 publications

(6 citation statements)

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“…4,17−20 This study focuses on Halobacterium noricense DSM 15987 T , which was originally isolated from a bore core of a salt mine in Altaussee, Austria. 17 Closely related strains and species were also isolated from other ancient rock salts, 4,20 suggesting this Halobacterium species would be a good representative of a rock salt indigenous archaeon.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In rock salt Haloarchaea represent a dominant part of the microbial community. 3,4 Microorganisms indigenous to those host rock environments have a potential impact on the mobility and retardation of radionuclides via, for example, biosorption, bioaccumulation, biotransformation, and biomineralization. 5−7 The formation of uranium-containing phosphate minerals by bacteria, fungi, and a few Archaea, was reported where metaautunite-like minerals were formed.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Therefore, the rock salt has a low intrinsic capacity to retard uranium. In rock salt Haloarchaea represent a dominant part of the microbial community. , Microorganisms indigenous to those host rock environments have a potential impact on the mobility and retardation of radionuclides via, for example, biosorption, bioaccumulation, biotransformation, and biomineralization. − The formation of uranium-containing phosphate minerals by bacteria, fungi, and a few Archaea, was reported where meta-autunite-like minerals were formed. − Thus far, only a limited number of studies have been reported about the biosorption of actinides on halophilic archaea. Sorption of Cm(III) onto cells of Halobacterium salinarum ATCC 19700 T was shown to be a fast process, which reached equilibrium after 20 min .…”

Section: Introductionmentioning

confidence: 99%

“…Extremely halophilic Archaea belonging to the genus Halobacterium were detected by both cultivation-dependent and cultivation-independent studies in rock salt worldwide. ,− This study focuses on Halobacterium noricense DSM 15987 T , which was originally isolated from a bore core of a salt mine in Altaussee, Austria . Closely related strains and species were also isolated from other ancient rock salts, , suggesting this Halobacterium species would be a good representative of a rock salt indigenous archaeon.…”

Section: Introductionmentioning

confidence: 99%

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Impact of Haloarchaea on Speciation of Uranium—A Multispectroscopic Approach

Bader

Roßberg

Steudtner

et al. 2018

Environ. Sci. Technol.

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Haloarchaea represent a predominant part of the microbial community in rock salt, which can serve as host rock for the disposal of high level radioactive waste. However, knowledge is missing about how Haloarchaea interact with radionuclides. Here, we used a combination of spectroscopic and microscopic methods to study the interactions of an extremely halophilic archaeon with uranium, one of the major radionuclides in high level radioactive waste, on a molecular level. The obtained results show that Halobacterium noricense DSM 15987 influences uranium speciation as a function of uranium concentration and incubation time. X-ray absorption spectroscopy reveals the formation of U(VI) phosphate minerals, such as meta-autunite, as the major species at a lower uranium concentration of 30 μM, while U(VI) is mostly associated with carboxylate groups of the cell wall and extracellular polymeric substances at a higher uranium concentration of 85 μM. For the first time, we identified uranium biomineralization in the presence of Halobacterium noricense DSM 15987 cells. These findings highlight the potential importance of Archaea in geochemical cycling of uranium and their role in biomineralization in hypersaline environments, offering new insights into the microbe-actinide interactions in highly saline conditions relevant to the disposal of high-level radioactive waste as well as bioremediation.

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Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of Haloarchaea on Speciation of Uranium—A Multispectroscopic Approach

Bader

Roßberg

Steudtner

et al. 2018

Environ. Sci. Technol.

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show abstract

“…A review of the literature relevant to the WIPP microbial colloid model has recently been performed and documented in Reed et al (2013a) and therefore is not repeated here. In that report, studies involving high-ionic-strength water are cited, including McGenity et al (2000), Gruber et al (2004), Ams et al (2013), Swanson et al (2012), , and Swanson and Simmons (2013). The studies pertain to the types of microbes expected in the WIPP environment, the potential attachment of actinides to those microbes, and the abundance of those microbes.…”

Section: Literature Reviewmentioning

confidence: 99%

Analysis report for WIPP colloid model constraints and performance assessment parameters

Mariner

Sassani

2014

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An analysis of the Waste Isolation Pilot Plant (WIPP) colloid model constraints and parameter values was performed. The focus of this work was primarily on intrinsic colloids, mineral fragment colloids, and humic substance colloids, with a lesser focus on microbial colloids. Comments by the US Environmental Protection Agency (EPA) concerning intrinsic Th(IV) colloids and Mg-Cl-OH mineral fragment colloids were addressed in detail, assumptions and data used to constrain colloid model calculations were evaluated, and inconsistencies between data and model parameter values were identified. This work resulted in a list of specific conclusions regarding model integrity, model conservatism, and opportunities for improvement related to each of the four colloid types included in the WIPP performance assessment.

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Plutonium interaction studies with the Mont Terri Opalinus Clay isolate Sporomusa sp. MT-2.99: changes in the plutonium speciation by solvent extractions

Moll

Cherkouk

Bok

et al. 2017

Environ Sci Pollut Res

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Since plutonium could be released from nuclear waste disposal sites, the exploration of the complex interaction processes between plutonium and bacteria is necessary for an improved understanding of the fate of plutonium in the vicinity of such a nuclear waste disposal site. In this basic study, the interaction of plutonium with cells of the bacterium, Sporomusa sp. MT-2.99, isolated from Mont Terri Opalinus Clay, was investigated anaerobically (in 0.1 M NaClO) with or without adding Na-pyruvate as an electron donor. The cells displayed a strong pH-dependent affinity for Pu. In the absence of Na-pyruvate, a strong enrichment of stable Pu(V) in the supernatants was discovered, whereas Pu(IV) polymers dominated the Pu oxidation state distribution on the biomass at pH 6.1. A pH-dependent enrichment of the lower Pu oxidation states (e.g., Pu(III) at pH 6.1 which is considered to be more mobile than Pu(IV) formed at pH 4) was observed in the presence of up to 10 mM Na-pyruvate. In all cases, the presence of bacterial cells enhanced removal of Pu from solution and accelerated Pu interaction reactions, e.g., biosorption and bioreduction.

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Status Report on the Microbial Characterization of Halite and Groundwater Samples from the WIPP

Cited by 7 publications

References 0 publications

Impact of Haloarchaea on Speciation of Uranium—A Multispectroscopic Approach

Impact of Haloarchaea on Speciation of Uranium—A Multispectroscopic Approach

Analysis report for WIPP colloid model constraints and performance assessment parameters

Plutonium interaction studies with the Mont Terri Opalinus Clay isolate Sporomusa sp. MT-2.99: changes in the plutonium speciation by solvent extractions

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