We describe the isolation and development of ten polymorphic microsatellite loci for western brook lamprey (Lampetra richardsoni). Two to nine alleles were observed per locus in a sample of 35 fish. Gene diversity ranged from 0.000 to 0.792 in six populations. Crossspecies amplification was successful as nine, seven and eight loci were polymorphic in Lampetra pacifica, Entosphenus hubbsi, and Entosphenus tridentatus, respectively. These markers will aid in evaluating the population structure of lamprey that are native to western North America.