Status of the executioner step of apoptosis in human with normal spermatogenesis and azoospermia

Bözec, Aline; Amara, Souheila; Guarmit, Basma; Selva, J.; Albert, Martine; Rollet, J; Sirkasi, Mohamed El; Vialard, François; Bailly, M.; Benahmed, Mohamed; Mauduit, Claire

doi:10.1016/j.fertnstert.2007.09.058

Cited by 18 publications

(16 citation statements)

References 30 publications

(56 reference statements)

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“…On the contrary, another study showed increased immunostaining of active caspase-3 in spermatocytes or spermatocytes and spermatids in cases of incomplete [4] and complete maturation arrest [14]. These discordant results might be due to differences in MA histology as well as to differences in germ cell quantification and analysis.…”

Section: Discussionmentioning

confidence: 96%

“…However, the apoptotic mechanisms involved are still poorly understood. Increased rates of DNA fragmentation and active caspase-3 were found in testes from patients with SCOS and maturation arrest [16] and decreased rates of TUNEL-positive germ cells were found in hypospermatogenesis, CBAVD and secondary obstruction cases [4]. However, different studies present discordant results [28].…”

Section: Discussionmentioning

confidence: 99%

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Caspase signalling pathways in human spermatogenesis

Almeida

Correia

Rocha

et al. 2013

J Assist Reprod Genet

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Purpose Little is known about the apoptotic mechanisms involved in abnormal spermatogenesis. In order to describe the significance of apoptosis in azoospermia, testicular tissue from abnormal spermatogenesis was analysed. Methods Testicular treatment biopsies were obtained from 27 men. Five presented oligozoospermia, 9 obstructive azoospermia (4 congenital bilateral absence of the vas deferens; 5 secondary azoospermia) and 13 nonobstructive azoospermia (5 hypospermatogenis; 3 maturation arrest; 5 Sertoli-cell-only syndrome). Immunohistochemical staining was performed for active caspases-3, −8 and −9. The presence of active caspases in Sertoli cells and germ cells was analyzed using stereological tools. Results Increased active caspase-3 was found in Sertoli-cellonly syndrome. No significant differences were found in maturation arrest. In hypospermatogenesis, primary spermatocytes were the germ cells with higher active caspases. Oligozoospermia and secondary obstruction showed significant differences among germ cells for the presence of all active caspases. In oligozoospermia, spermatogonia presented significant increased active caspase-9 in relation to active caspase-8. In primary obstruction and hypospermatogenesis, germ cells presented significant increased active caspases-3 and −9. Conclusions Results suggest that increased active caspase-3 might be involved in Sertoli-cell-only syndrome etiology. In cases of hypospermatogenesis, intrinsic lesions at the meiotic stage seem to be related to the pathology. In secondary obstruction apoptosis is suggested to be initiated due to extrinsic and intrinsic lesions, whereas in primary obstruction only the intrinsic apoptotic pathway seems to be present. Finally, in oligozoospermic patients spermatogonia death by mitochondrial damage additionally to meiosis malfunctioning, might be on the origin of the decreased sperm output.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Caspase signalling pathways in human spermatogenesis

Almeida

Correia

Rocha

et al. 2013

J Assist Reprod Genet

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“…Accordingly, altered expression patterns of a panoply of apoptosis-related genes have been described in human testes with defective spermatogenesis. The association of death receptors and the extrinsic pathway of apoptosis with male infertility have been suggested by several studies, which showed enhanced expression of FasL in cases of maturation arrest and Sertoli cell-only syndrome, the later characterised by the absence of germ cell in the seminiferous epithelium ( Refs 5,34,35,36,37,38). Cavalcanti et al found an increased expression of tumour necrosis factor alpha (TNF-α) family member 10 (also known as TNF-α-related apoptosis inducing ligand, TRAIL) in the testes of subfertile men comparatively with potentially fertile (Ref.…”

Section: Clinical Findings Of Deregulated Apoptosis In the Testis Of mentioning

confidence: 99%

Oestrogens as apoptosis regulators in mammalian testis: angels or devils?

Correia

Cardoso

Cavaco

et al. 2015

Expert Rev. Mol. Med.

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In the mammalian testis, spermatogenesis is a highly coordinated process of germ cell development, which ends with the release of 'mature' spermatozoa. The fine regulation of spermatogenesis is strictly dependent on sex steroid hormones, which orchestrate the cellular and molecular events underlying normal development of germ cells. Sex steroids actions also rely on the control of germ cell survival, and the programmed cell death by apoptosis has been indicated as a critical process in regulating the size and quality of the germ line. Recently, oestrogens have emerged as important regulators of germ cell fate. However, the beneficial or detrimental effects of oestrogens in spermatogenesis are controversial, with independent reports arguing for their role as cell survival factors or as apoptosis-inducers. The dual behaviour of oestrogens, shifting from 'angels to devils' is supported by the clinical findings of increased oestrogens levels in serum and intratesticular milieu of idiopathic infertile men. This review aims to discuss the available information concerning the role of oestrogens in the control of germ cell death and summarises the signalling mechanisms driven oestrogen-induced apoptosis. The present data represent a valuable basis for the clinical management of hyperoestrogenism-related infertility and provide a rationale for the use of oestrogen-target therapies in male infertility.

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“…The role of executioner caspases in impaired spermatogenesis is well documented (9). Further, the expression of p53 was always a question of debate because its expression was increased in early spermatogenesis (10), and p53 knockout exhibited more undifferentiated spermatogonia compared with wild-type mice (11).…”

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confidence: 99%