Status of the current vitivirus taxonomy

Maree, Hans J.; Blouin, Arnaud; Diaz‐Lara, Alfredo; Mostert, Ilani; Rwahnih, Maher Al; Candresse, Thierry

doi:10.1007/s00705-019-04500-w

Cited by 22 publications

(28 citation statements)

References 44 publications

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“…In our GLD_S++ data, the total number of GVB reads represented more than 62% of all viral reads. This increased vitivirus titer may be the reason why vitivirus transmission is facilitated when the donor plant is also infected by a leafroll virus [26][27][28]. Virus distribution will vary between generative and vegetative organs as well as with respect to the berry developmental stage, and this analysis only relied on sequencing data of veraison and mid-ripening berries.…”

Section: Discussionmentioning

confidence: 99%

Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years

Reynard

Brodard

Zufferey

et al. 2022

Viruses

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Grapevine leafroll disease (GLD) is one of the most economically damaging virus diseases in grapevine, with grapevine leafroll-associated virus 1 (GLRaV-1) and grapevine leafroll-associated virus 3 (GLRaV-3) as the main contributors. This study complements a previously published transcriptomic analysis and compared the impact of two different forms of GLD to a symptomless control treatment: a mildly symptomatic form infected with GLRaV-1 and a severe form with exceptionally early leafroll symptoms (up to six weeks before veraison) infected with GLRaV-1 and GLRaV-3. Vine physiology and fruit composition in 17-year-old Pinot noir vines were measured and a gradient of vigor, yield, and berry quality (sugar content and berry weight) was observed between treatments. Virome composition, confirmed by individual RT-PCR, was compared with biological indexing. Three divergent viromes were recovered, containing between four to seven viruses and two viroids. They included the first detection of grapevine asteroid mosaic-associated virus in Switzerland. This virus did not cause obvious symptoms on the indicators used in biological indexing. Moreover, the presence of grapevine virus B (GVB) did not cause the expected corky bark symptoms on the indicators, thus underlining the important limitations of the biological indexing. Transmission of GLRaV-3 alone or in combination with GVB by Planococcus comstocki mealybug did not reproduce the strong symptoms observed on the donor plant infected with a severe form of GLD. This result raises questions about the contribution of each virus to the symptomatology of the plant.

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Section: Discussionmentioning

confidence: 99%

Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years

Reynard

Brodard

Zufferey

et al. 2022

Viruses

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“…Although Vitis spp. is recognized as the main host associated with the genus Vitivirus, vitiviruses have been identified in other perennial hosts, the majority of which are woody plants [9]. For example, vitiviruses have been reported in blackberry, mint, agave and recently in blueberry.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, a field survey was conducted of known vitivirus-infected grapevines. Following the first reports of vitiviruses in grapevine, several vitiviruses have been discovered in other hosts [9]; consequently, we investigated if the universal assay can detect these vitiviruses.…”

Section: Introductionmentioning

confidence: 99%

Development of a universal RT-PCR assay for grapevine vitiviruses

et al. 2020

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The genus Vitivirus in the family Betaflexiviridae includes eleven viruses known to infect grapevine: grapevine vitiviruses A, B, D, E, F, G, H, I, J, L and M (GVA-GVM). Three of these viruses, GVA, GVB and GVD, have been associated with the etiology of rugose wood disease in grapevine and cause agronomically significant losses. The other vitiviruses were more recently discovered and their effects on grapevine are undetermined. To certify grape material for propagation as virus tested, an updated reverse transcription PCR (RT-PCR) assay to detect all known vitiviruses is desirable. To accomplish this, multiple grapevine vitivirus sequences were aligned at the amino acid level to search for conserved motifs. Two highly conserved motifs were found at an ideal distance for RT-PCR detection in the RNAdependent RNA polymerase region of the replicase protein. The amino acid motifs were back translated to create degenerate primers and used to successfully amplify all eleven grapevine vitiviruses. The RT-PCR primers were used to test a panel of vitivirus-infected vines for inclusivity as well as vines infected with closely related viruses in the Betaflexiviridae family (i.e. grapevine pinot gris virus and grapevine rupestris stem pitting-associated virus) for exclusivity. Broader use of these primers to detect vitiviruses in other plant hosts was investigated. In summary, an end-point RT-PCR assay that detects all the known grapevine vitiviruses and potentially other members of the genus Vitivirus has been developed. The universal assay represents an alternative to individual assays to reduce the work associated with the diagnosis of vitiviruses, including for regulatory purposes.

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“…The genus Vitivirus was initially created to accommodate grapevine viruses sharing similar genome features and organization. It now also includes viruses infecting other hosts and today counts 15 species mostly identi ed from woody or perennial hosts [2]. Additional species in the genus have also been reported that have yet to be recognized by the International Committee on Taxonomy of Viruses (ICTV; [2]).…”

Section: Annotated Sequence Recordmentioning

confidence: 99%