Status of Sensitivity of Northern German Botrytis Populations to the New Sdhi Fungicide Fluopyram Prior to its Release as a Commercial Fungicide

Weber, Roland W.S.; Entrop, Alfred-Peter; Goertz, Andreas; Mehl, Andreas

doi:10.1007/bf03356535

Cited by 23 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our main conclusions are that MR strains are likely to possess a high fitness in the field and that they are essentially immune to sprays with any of the current botryticides, with the possible exception of the recently released fluopyram (Weber et al, 2015). This has obvious implications for practical horticultural production.…”

Section: Discussionmentioning

confidence: 91%

“…Active ingredients were obtained by using single-compound fungicides, as follows: fenhexamid from Teldor (Bayer CropScience, Monheim, Germany); trifloxystrobin from Flint (Bayer) or azoxystrobin from Ortiva (Syngenta, Basel, Switzerland); boscalid from Cantus (BASF, Limburgerhof, Germany); cyprodinil from Chorus (Syngenta); fludioxonil from Saphire (Syngenta); iprodione from Rovral WG (BASF) and thiophanate-methyl from Cercobin FL (BASF). The SDHI compound fluopyram was also sometimes tested but data are not reported here because this compound was not registered until the 2015 season (Weber et al, 2015). …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Spread of Botrytis cinerea Strains with Multiple Fungicide Resistance in German Horticulture

Rupp

Weber

Rieger³

et al. 2017

Front. Microbiol.

140

View full text Add to dashboard Cite

Botrytis cinerea is a major plant pathogen, causing gray mold rot in a variety of cultures. Repeated fungicide applications are common but have resulted in the development of fungal populations with resistance to one or more fungicides. In this study, we have monitored fungicide resistance frequencies and the occurrence of multiple resistance in Botrytis isolates from raspberries, strawberries, grapes, stone fruits and ornamental flowers in Germany in 2010 to 2015. High frequencies of resistance to all classes of botryticides was common in all cultures, and isolates with multiple fungicide resistance represented a major part of the populations. A monitoring in a raspberry field over six seasons revealed a continuous increase in resistance frequencies and the emergence of multiresistant Botrytis strains. In a cherry orchard and a vineyard, evidence of the immigration of multiresistant strains from the outside was obtained. Inoculation experiments with fungicide-treated leaves in the laboratory and with strawberry plants cultivated in the greenhouse or outdoors revealed a nearly complete loss of fungicide efficacy against multiresistant strains. B. cinerea field strains carrying multiple resistance mutations against all classes of site-specific fungicides were found to show similar fitness as sensitive field strains under laboratory conditions, based on their vegetative growth, reproduction, stress resistance, virulence and competitiveness in mixed infection experiments. Our data indicate an alarming increase in the occurrence of multiresistance in B. cinerea populations from different cultures, which presents a major threat to the chemical control of gray mold.

show abstract

Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Spread of Botrytis cinerea Strains with Multiple Fungicide Resistance in German Horticulture

Rupp

Weber

Rieger³

et al. 2017

Front. Microbiol.

140

View full text Add to dashboard Cite

show abstract

“…The most common mutations detected in sdhB were at codon 272, of which the majority resulted in H272R, followed by H272Y, both of which confer resistance to boscalid and sensitivity to fluopyram (Veloukas et al, 2013;Weber et al, 2015). However, we also detected N230I and P225F, which confer resistance to both boscalid and fluopyram (Veloukas et al, 2013;Weber et al, 2015). b Spore germination test, with resistance categories resistant (R), moderately resistant (mR), less sensitive (s) and highly sensitive (ss), according to Weber and Hahn (2011).…”

Section: Discussionmentioning

confidence: 99%

Pervasive fungicide resistance in Botrytis from strawberry in Norway: Identification of the grey mould pathogen and mutations

et al. 2022

View full text Add to dashboard Cite

Control of grey mould, caused by Botrytis spp., is a major challenge in open field strawberry production. Botrytis was isolated from plant parts collected from 19 perennial strawberry fields with suspected fungicide resistance in the Agder region of Norway in 2016. Resistance to boscalid, pyraclostrobin and fenhexamid was high and found in 89.1%, 86.0% and 65.4% of conidia samples, respectively. Multiple fungicide resistance was common; 69.6% of conidia samples exhibited resistance to three or more fungicides. Botrytis group S and B. cinerea sensu stricto isolates were obtained from 19 and 16 fields, respectively. The sdhB, cytb, erg27 and mrr1 genes of a selection of isolates were examined for the presence of mutations known to confer fungicide resistance to boscalid, pyraclostrobin, fenhexamid and pyrimethanil plus fludioxonil, respectively. Allele-specific PCR assays were developed for efficient detection of resistance-conferring mutations in cytb. Among B. cinerea isolates, 84.7%, 86.3% and 61.3% had resistance-conferring mutations in sdhB, cytb and erg27, respectively. A triplet deletion in mrr1, resulting in ΔL497, commonly associated with the multidrug resistance phenotype MDR1h, was detected in 29.2% of Botrytis group S isolates.High frequencies of resistance to several fungicides were also detected in Botrytis from both imported and domestically produced strawberry transplants. Fungicide resistance frequencies were not different among fields grouped by level of grey mould problem assessed by growers, indicating factors other than fungicide resistance contributed to control failure, a fact that has important implications for future management of grey mould.

show abstract

“…In relation to P. xanthii , similar frequencies were described for boscalid in the American and Japanese populations of this pathogen, with 44 and 45.96% resistant isolates, respectively [ 30 , 58 ]. Different studies on B. cinerea showed approximately 50% boscalid-resistant isolates for the total population in Greece, Germany, and Spain [ 23 , 27 , 59 ]. With regard to a study developed in several strawberry fields in Spain, an increase from 5.3% to 10.4% in fluopyram-resistant isolates was observed in the B. cinerea population collected between 2015 and 2016 [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Resistance to the SDHI Fungicides Boscalid and Fluopyram in Podosphaera xanthii Populations from Commercial Cucurbit Fields in Spain

Vielba-Fernández

Polonio

Ruiz-Jiménez

et al. 2021

JoF

View full text Add to dashboard Cite

Powdery mildew is caused by Podosphaera xanthii, and is one of the most important diseases that attacks Spanish cucurbit crops. Fungicide application is the primary control tool; however, its effectiveness is hampered by the rapid development of resistance to these compounds. In this study, the EC50 values of 26 isolates were determined in response to the succinate dehydrogenase inhibitor (SDHI) fungicides boscalid and fluopyram. From these data, the discriminatory doses were deduced and used for SDHI resistance monitoring during the 2018 and 2019 growing seasons. Of the 298 isolates analysed, 37.9% showed resistance to boscalid and 44% to fluopyram. Although different phenotypes were observed in leaf disc assays, the resistant isolates showed the same phenotype in plant assays. Compared to sensitive isolates, two amino acid changes were found in the SdhC subunit, A86V and G151R, which are associated mostly with resistance patterns to fluopyram and boscalid, respectively. Furthermore, no significant differences were observed in terms of fitness cost between the selected sensitive and resistant isolates analysed here. Lastly, a loop-mediated isothermal amplification (LAMP) assay was developed to detect A86V and G151R mutations using conidia obtained directly from infected material. Our results show that growers could continue to use boscalid and fluopyram, but resistance management practices must be implemented.

show abstract

Status of Sensitivity of Northern German Botrytis Populations to the New Sdhi Fungicide Fluopyram Prior to its Release as a Commercial Fungicide

Cited by 23 publications

References 28 publications

Spread of Botrytis cinerea Strains with Multiple Fungicide Resistance in German Horticulture

Spread of Botrytis cinerea Strains with Multiple Fungicide Resistance in German Horticulture

Pervasive fungicide resistance in Botrytis from strawberry in Norway: Identification of the grey mould pathogen and mutations

Resistance to the SDHI Fungicides Boscalid and Fluopyram in Podosphaera xanthii Populations from Commercial Cucurbit Fields in Spain

Contact Info

Product

Resources

About