Status of orchid viruses in India and management strategies for them

Pant, R. P.; Rashmi, E. R.; Manjunath, N.; Baranwal, V. K.

doi:10.1016/b978-0-12-818654-1.00051-7

Cited by 7 publications

(3 citation statements)

References 133 publications

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“…Historically, transmission electron microscopy was the first method used to study MNPs [206,207]. Subsequently, researchers conducted direct size measurements of MNPs in the magnetic fluid using scanning electron microscopy [208,209], atomic force microscopy [210,211], and magneto-force microscopy [212].…”

Section: Experimental Methods For Studying the Structure Properties A...mentioning

confidence: 99%

Magnetic Fluids: The Interaction between the Microstructure, Macroscopic Properties, and Dynamics under Different Combinations of External Influences

Ryapolov,

Vasilyeva,

Kalyuzhnaya

et al. 2024

Nanomaterials

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Magnetic fluids were historically the first active nano-dispersion material. Despite over half a century of research, interest in these nano-objects continues to grow every year. This is due to the impressive development of nanotechnology, the synthesis of nanoscale structures, and surface-active systems. The unique combination of fluidity and magnetic response allows magnetic fluids to be used in engineering devices and biomedical applications. In this review, experimental results and fundamental theoretical approaches are systematized to predict the micro- and macroscopic behavior of magnetic fluid systems under different external influences. The article serves as working material for both experienced scientists in the field of magnetic fluids and novice specialists who are just beginning to investigate this topic.

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Section: Experimental Methods For Studying the Structure Properties A...mentioning

confidence: 99%

Magnetic Fluids: The Interaction between the Microstructure, Macroscopic Properties, and Dynamics under Different Combinations of External Influences

Ryapolov,

Vasilyeva,

Kalyuzhnaya

et al. 2024

Nanomaterials

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“…CymMV has flexuous filamentous particle morphology with a single species of linear positive sense single stranded RNA. The first complete sequence of CymMV genome came from Singapore [ 10 , 13 ]. The nucleic acid length of genome is about 6 kb coding for five proteins, including the RNA-dependant RNA polymerase ( RdRp ), triple gene block 1 ( TGB1 ), triple gene block 2 ( TGB2 ), triple gene block 3 ( TGB3 ) and Coat protein ( CP ) [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Annual Accumulation of CymMV May Lead to Loss in Production of Asymptomatic Vanilla Propagated by Cuttings

Gu,

Xu,

et al. 2024

Plants

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Vanilla (Vanilla planifolia Andrews) is a valuable orchid spice cultivated for its highly priced beans. Vanilla has been planted in Hainan province of China via cutting propagation for about 40 years. The yield has been decreasing annually for the past ten years due to pod numbers declining significantly even though it seems to grow normally without disease symptoms, while the reason is still unknown. In this study, we found that Cymbidium mosaic virus (CymMV), one of the most devastating viruses causing losses in the vanilla industry, massively presented within the pods and leaves of vanilla plants, so the virus infecting the vanilla seems to be a highly probable hypothesis of the main contributions to low yield via decreasing the number of pods. This represents the first speculation of CymMV possibly affecting the yield of vanilla in China, indicating the important role of virus elimination in restoring high yield in vanilla. This research can also serve as a warning to important economic crops that rely on cuttings for propagation, demonstrating that regular virus elimination is very important for these economically propagated crops through cuttings.

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“…Orchids are significant ornamental plants from the Orchidaceae family, representing economically popular ornamental cut-flowers and potted floricultural crops worldwide (Ali R. N. et al, 2014;Yusop et al, 2022). However, more than 50 viruses are known to infect orchids globally, and cymbidium mosaic virus (CymMV) and odontoglossum ringspot virus (ORSV) are the two most prevalent and economically essential orchid viruses (Wong et al, 1994;Pant et al, 2020;Jia et al, 2004) that achieved a worldwide distribution, infecting numerous commercially significant orchid genera (Zettler et al, 1990;Jia et al 2004). Cymbidium ringspot virus (CymRSV) is a virus of serious concern that infects orchids, primarily in Europe, North America, South America, and other locations (Medzihradszky et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Establishment of a triplex TaqMan quantitative real-time PCR assay for simultaneous detection of Cymbidium mosaic virus, Odontoglossum ringspot virus and Cymbidium ringspot virus

et al. 2023

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Orchids are significant ornamental plants whose viral infection results in substantial economic damage. Cymbidium mosaic virus (CymMV), Odontoglossum ringspot virus (ORSV), and Cymbidium ringspot virus (CymRSV) represent three important and prevalent orchid viruses. The detection system proposed in this study uses a triplex TaqMan quantitative real-time PCR assay to identify CymMV, ORSV, and CymRSV in a simultaneous manner. We designed specific primers and probes for CymMV, ORSV, and CymRSV, with amplified sequences of 156 bp, 148 bp, and 145 bp, respectively. The minimum detection limit of the triplex qRT-PCR assay for CymMV and CymRSV was 1 copy/assay, and the minimum detection limit was 10 copies/assay for ORSV. The minimum stable detection limits for CymMV, ORSV, and CymRSV were 10, 102, and 102 copies/assay, respectively. Therefore, this system exhibited higher sensitivity (approximately 10 to 104-fold) than RT–PCR. The intra-and interassay CVs of Cq values are less than 0.55 and 0.95%, respectively, indicating that the triplex assay is highly reliable and accurate. In addition, 66 samples from five different orchid genera were analyzed using the established assay and gene chip. The detection results demonstrated that the triplex probe qRT–PCR demonstrated higher sensitivity than the gene chip, indicating that the triplex real-time PCR assay could be used for the detection of field samples. Our findings suggest that the triplex real-time RT–PCR detection system represents a rapid, simple, and accurate tool for detecting CymMV, ORSV, and CymRSV on orchids.

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Status of orchid viruses in India and management strategies for them

Cited by 7 publications

References 133 publications

Magnetic Fluids: The Interaction between the Microstructure, Macroscopic Properties, and Dynamics under Different Combinations of External Influences

Magnetic Fluids: The Interaction between the Microstructure, Macroscopic Properties, and Dynamics under Different Combinations of External Influences

Annual Accumulation of CymMV May Lead to Loss in Production of Asymptomatic Vanilla Propagated by Cuttings

Establishment of a triplex TaqMan quantitative real-time PCR assay for simultaneous detection of Cymbidium mosaic virus, Odontoglossum ringspot virus and Cymbidium ringspot virus

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