Status of methods for assessing bacterial cell surface charge properties based on zeta potential measurements

Wilson, W. William; Wade, Mary Margaret; Holman, Steven C.; Champlin, Franklin R.

doi:10.1016/s0167-7012(00)00224-4

Cited by 508 publications

(339 citation statements)

References 75 publications

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“…This was caused by domination of well-dissociated acidic functional groups (e.g., carboxyl, phosphoryl or sulfhydryl groups of carbohydrates and proteins) on the surface and the location of counterions far from the bacteria/liquid interface (Rutter and Vincent 1984;Wilson et al 2001). In the case of R. leguminosarum bv.…”

Section: Discussionmentioning

confidence: 99%

“…In the case of R. leguminosarum bv. trifolii, sources of the net negative electrical charge of cells are mainly phosphoryl and 3-deoxy-D-mannooctulosonic acid (Kdo) carboxylate groups of lipopolysaccharide located in the outer bacterial membrane (Bushby 1990;Wilson et al 2001). Also, other polysaccharides synthesized by rhizobia (e.g., EPS, gel-forming polysaccharide and glucomannan) could modify their surface charge and, in consequence, attachment to different materials, but this has not been investigated so far.…”

Section: Discussionmentioning

confidence: 99%

“…The methodology used in this study was consistent with that presented in the paper by Cieśla et al (2011). The zeta potential values were calculated by the software on the basis of the Henry equation with the use of the Smoluchowski approximation (Delgado et al 2007;Wilson et al 2001). All measurements were done at 20°C (±0.1).…”

Section: Determination Of the Electrokinetic Potential Of Rhizobial Smentioning

confidence: 99%

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Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties

et al. 2014

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Background and aims The acidic exopolysaccharide (EPS) produced by Rhizobium leguminosarum bv. trifolii is required for the establishment of effective symbiosis with compatible host plants (Trifolium spp.). In the rhizobium-legume interaction, early stages of root infection and nodule development have been well studied from a genetic standpoint. However, factors important for colonization of several surfaces by rhizobia, including soil particles and roots, have not yet been thoroughly investigated. The aim of this study was establishing of environmental factors affecting production of EPS by R. leguminosarum bv. trifolii strain 24.2 and the role of this polysaccharide in bacterial surface properties and attachment ability. Methods Besides the wild-type strain, its derivatives differing in the level of EPS produced were used to these analyses. The ability of attachment to abiotic and biotic surfaces of these strains were established using CFU counting experiments. Three-dimensional structure and other parameters of biofilms formed were characterized in confocal laser scanning microscopy. Electrokinetic (zeta) potential of rhizobial cells were determined using Laser Doppler Velocimetry. Results It was evidenced that the ability of R. leguminosarum bv. trifolii to produce EPS significantly affected bacterial attachment and biofilm formation on both abiotic and biotic surfaces. In addition, the presence of this polysaccharide influenced the zeta potential of rhizobial cells. Mutant strains having a mutation in genes involved in EPS synthesis were significantly impaired in attachment, whereas strains overproducing this polysaccharide showed higher adhesion efficiency to all of the tested materials. EPS facilitated attachment of bacterial cells to the tested surfaces most probably due to hydrophobic interactions and heterogeneity of the envelope surface. Conclusions EPS produced by R. leguminosarum bv. trifolii plays a significant role in attachment and biofilm formation to both abiotic and biotic surfaces as well as bacterial surface properties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Determination Of the Electrokinetic Potential Of Rhizobial Smentioning

confidence: 99%

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Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties

et al. 2014

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“…However, most of these studies investigated the use of the technique for detecting and/or quantifying the food-borne bacteria based on the electrophysiological and impedance properties of biological cells. Besides, the conductivity of bacterial suspensions has also been reported to study the electrical properties of bacterial cell surface and related cell surface interfacial physiology [15,16], but fewer studies have been reported to quantify the concentration of bacterial cells in the suspensions using the impedance-based method. Wheeler and Goldschmidt [17] found a positive relationship between the cell concentration of E. coli and the voltage changes in the suspension.…”

mentioning

confidence: 99%

Development of a novel conductance-based technology for environmental bacterial sensing

Shen

Tan

et al. 2013

Chin. Sci. Bull.

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In this study, a simple impedance based technology for measuring bacterial concentrations was developed. The measurement system includes the signal amplification, copper probes and a sample loader. During the experiments, the conductance of Bacillus subtilis var niger, Pseudomonas fluorescens, and Escherichia coli were measured using the combination of a pre-amplifier and a lock-in amplifier. The conductance data were modeled verses the bacterial concentrations. Results indicated that the relationship between the conductance of bacterial suspensions and their concentrations follows a generic model: Y=C 1 + C 2 × e (−X/C 3 ) , where Y is the conductance (S), X is the bacterial concentration (Number/mL: abbreviated to N/mL) for all species tested, and C 1−3 are constants. Gram negative P. fluorescens and E. coli assumed similar conductance curves, which were flatter than that of gram positive B. subtilis var niger. For P. fluorescens and E. coli the culturing technique resulted in higher concentration levels (statistically significant) from 2 to 4 times that measured by the impedance based technology. For B. subtilis var niger, both methods resulted in similar concentration levels. These differences might be due to membrane types, initial culturability and the obtained conductance curves. The impedance based technology here was shown to obtain the bacterial concentration instantly, holding broad promise in realtime monitoring biological agents.

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“…Multivariate methods were used to identify relationships between bacterial attachment, water transport, and the surface properties of modified polysulfone (MPS) membranes, which showed that the interrelations were quite complicated [137]. In summary, the research into modification of surface topography to achieve antifouling is still at the experimental stage.…”

Section: Antifouling By Modification Of Surface Topography and Hydropmentioning

confidence: 99%

Progress of marine biofouling and antifouling technologies

et al. 2010

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Status of methods for assessing bacterial cell surface charge properties based on zeta potential measurements

Cited by 508 publications

References 75 publications

Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties

Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties

Development of a novel conductance-based technology for environmental bacterial sensing

Progress of marine biofouling and antifouling technologies

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