Status of carbohydrate, protein and lipid profile in the mosquito larvae treated with certain phytoextracts

Sharma, Preeti; Mohan, Lalit; Dua, Kamal; Srivastava, C.N.

doi:10.1016/s1995-7645(11)60090-4

Cited by 45 publications

(29 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Sharma et al . (), the decrease in the protein levels in the P. nigrispinus nymphs caused by the botanical insecticide is probably due to the interference to the hormones that regulate protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…According to Sharma et al (2011), the decrease in the protein levels in the P. nigrispinus nymphs caused by the botanical insecticide is probably due to the interference to the hormones that regulate protein synthesis. Therefore, the decrease in sugar and protein levels in the P. nigrispinus nymphs treated with azadirachtin shows that this insecticide is able to modify the biochemical profile of this predator.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Immune and nutritional responses of Podisus nigrispinus (Hemiptera: Pentatomidae) nymphs sprayed with azadirachtin

et al. 2019

View full text Add to dashboard Cite

Azadirachtin is an efficient biopesticide, and it is considered safe for natural enemies because its mode of action is mainly by ingestion. The predator, Podisus nigrispinus Dallas, is found in various agroecosystems as a potential tool for biological control. However, there is much debate on the safety of this stinkbug if azadirachtin is used. Thus, we evaluated the cellular and humoral responses as well as biochemical parameters of the fifth‐instar nymphs of P. nigrispinus treated with azadirachtin. The insects were divided into three treatment groups and sprayed with azadirachtin (Azamax®), deltamethrin (Decis®), and water (control). After 24 h, total and differential hemocyte counts were performed. The analysis of phenoloxidase activity and quantification of nitric oxide, lipids, sugar, glycogen, and protein were performed after an interval of 24 and 72 h. Azadirachtin was found to affect the immune system of this stinkbug and decrease the number of hemocytes, especially pro‐hemocytes and oenocytoids, and it may have caused a reduction in phenoloxidase activity and nitric oxide level. In addition, the decrease in protein levels indicated the interference of azadirachtin in protein synthesis. We conclude that damage caused by the botanical insecticide has been less harmful than that caused by the synthetic insecticide, but use of azadirachtin may still interfere with biological control due to undesirable immune system damage reducing predator efficiency.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immune and nutritional responses of Podisus nigrispinus (Hemiptera: Pentatomidae) nymphs sprayed with azadirachtin

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The Artemisia annua extract negatively affects the growth and the development of C. quinquefasciatus (Sharma et al, 2011). Plant extracts tested against larvae, and adults of An.…”

Section: Discussionmentioning

confidence: 99%

“…However, changes in the synthesis of proteins and total lipids after the exposure of the larvae of C. quinquefasciatus to imidacloprid have been observed (Rao et al 2008). Moreover, it was observed a significant change in the biochemical profile of the larvae of Anopheles stephensi and C. quinquefasciatus exposed to certain phytoextracts (Sharma et al 2011).…”

Section: Introductionmentioning

confidence: 97%

The Influence of Fatty Acid Methyl Esters (FAMEs) in the Biochemistry and the Na+/K+-ATPase Activity of Culex quinquefasciatus Larvae

et al. 2016

View full text Add to dashboard Cite

Culex quinquefasciatus is the main vector of lymphatic filariasis and combating this insect is of great importance to public health. There are reports of insects that are resistant to the products currently used to control this vector, and therefore, the search for new products has increased. In the present study, we have evaluated the effects of fatty acid methyl esters (FAMEs) that showed larvicidal activity against C. quinquefasciatus, on glucose, total protein, and triacylglycerol contents and Na(+)/K(+)-ATPase activity in mosquito larvae. The exposure of the fourth instar larvae to the compounds caused a decrease in the total protein content and an increase in the activity of the Na(+)/K(+)-ATPase. Furthermore, the direct effect of FAMEs on cell membrane was assessed on purified pig kidney Na(+)/K(+)-ATPase membranes, erythrocyte ghost membranes, and larvae membrane preparation. No modifications on total phospholipids and cholesterol content were found after FAMEs 20 min treatment on larvae membrane preparation, but only 360 µg/mL FAME 2 was able to decrease total phospholipid of erythrocyte ghost membrane. Moreover, only 60 and 360 µg/mL FAME 3 caused an activation of purified Na(+)/K(+)-ATPase, that was an opposite effect of FAMEs treatment in larvae membrane preparation, and caused an inhibition of the pump activity. These data together suggest that maybe FAMEs can modulate the Na(+)/K(+)-ATPase on intact larvae for such mechanisms and not for a direct effect, one time that the direct effect of FAMEs in membrane preparation decreased the activity of Na(+)/K(+)-ATPase. The biochemical changes caused by the compounds were significant and may negatively influence the development and survival of C. quinquefasciatus larvae.

show abstract

“…Furthermore, the carbohydrate reduction may be due to prohibiting effects of the tested IGRs on glycogen and/or trehalose or interference with the glycolytic path-way. On the other hand, it is suggested that this carbohydrate depletion may be due to the utilization of the reserved glucose sources of the larval tissues as a result of IGRs' stresses (Sharma et al, 2011).…”

Section: Disturbed Carbohydrate Content In P Unionalis By Igrsmentioning

confidence: 99%

Disturbing Effects of Three Insect Growth Regulators on General Body Metabolism of the Olive Leaf Moth, Palpita unionalis (Hübner) (Lepidoptera: Pyralidae)

Hamadah

Ghoneim

Aز

et al. 2017

Egyptian Academic Journal of Biological Sciences. C, Physiology

View full text Add to dashboard Cite

show abstract

Status of carbohydrate, protein and lipid profile in the mosquito larvae treated with certain phytoextracts

Cited by 45 publications

References 17 publications

Immune and nutritional responses of Podisus nigrispinus (Hemiptera: Pentatomidae) nymphs sprayed with azadirachtin

Immune and nutritional responses of Podisus nigrispinus (Hemiptera: Pentatomidae) nymphs sprayed with azadirachtin

The Influence of Fatty Acid Methyl Esters (FAMEs) in the Biochemistry and the Na+/K+-ATPase Activity of Culex quinquefasciatus Larvae

Disturbing Effects of Three Insect Growth Regulators on General Body Metabolism of the Olive Leaf Moth, Palpita unionalis (Hübner) (Lepidoptera: Pyralidae)

Contact Info

Product

Resources

About