Status of  and #945;-tocopherol concentration and oxidative stress in infertile females of Vijayapur District, northern Karnataka

Jargar, Jameel G.; Dhundasi, Salim A; Punekar, MeherDarakshan

doi:10.5455/njppp.2017.7.0516819052017

Cited by 4 publications

(3 citation statements)

References 20 publications

(36 reference statements)

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“…Vitamin E concentrations in serum samples were determined according to the existing protocol [ 34 ]. First, absolute ethanol was used to denature serum proteins and Xylene was used to separate the supernatant from proteins.…”

Section: Methodsmentioning

confidence: 99%

Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study

et al. 2023

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Objective: The present study aimed to evaluate the effects of enriched hen egg consumption on endothelium-dependent vasodilation (EDV) and the role of cyclooxygenases in EDV in the microcirculation of young healthy individuals. This study hypothesizes that Nutri4 eggs will improve endothelial function, which will be manifested by changes in microcirculatory flow measured by a laser Doppler flowmeter (LDF) during reactive hyperemia in response to vascular occlusion, in which n-3 PUFA plays an important role as well as its degradation pathway by cyclooxygenases. Materials and Methods: Participants consumed three eggs per day for three weeks: The control group (CTRL, n = 14) consumed regular hen eggs (approximately 0.330 mg of lutein, 1.785 mg of vitamin E, 0.054 mg of selenium and 438 mg of n-3 PUFAs daily) and Nutri4 group (n = 20) consumed enriched eggs (approximately 1.85 mg of lutein, 0.06 mg of selenium, 3.29 mg of vitamin E, and 1026 mg of n-3 PUFAs daily). Skin microvascular blood flow in response to EDV (post-occlusive reactive hyperemia (PORH) and iontophoresis of acetylcholine (AChID)) and sodium nitroprusside (SNPID; endothelium-independent) was assessed by laser Doppler flowmetry before and after dietary protocol and in a separate group of participants who were administered perorally 100 mg of indomethacin before microvascular response assessment. Arterial blood pressure, heart rate, serum lipid, and liver enzymes, anthropometric measurements, protein expression of cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), neuronal nitric oxide synthases (nNOS), inducible nitric oxide synthases (iNOS), and endothelial nitric oxide synthases (eNOS) were measured before and after dietary protocol. Results: PORH and AChID were significantly enhanced, and SNPID remained unchanged in the Nutri4 group, while none was changed in the CTRL following a respective diet. PORH decreased after administration of indomethacin in Nutri4 after dietary protocol. Protein expression of COX-2 was significantly higher in the Nutri4 group compared to the CTRL after the dietary protocol. Conclusion: Consumption of enriched eggs improves microvascular EDV in healthy young subjects. Results suggest an element of n-3 PUFAs metabolites via the cyclooxygenases pathway in enhanced reactive hyperemia.

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Section: Methodsmentioning

confidence: 99%

Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study

et al. 2023

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“…The controversy is on the differences in lifestyles and OS status of fertile and infertile women and the relationship between lifestyles and OS status in female infertility. A study [17] found no significant difference in body mass index (BMI) in contrast to another study findings [18]. In addition, two studies [19,20] found no significant differences in OS status while some studies [21][22][23] found significant differences between the two groups.…”

Section: Introductionmentioning

confidence: 93%

Lifestyle and oxidative stress status in infertile women in Dar es Salaam, Tanzania: comparative cross-sectional study

Kasililika

Odukogbe

Dairo

et al. 2021

Middle East Fertil Soc J

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Background Female infertility is a public health problem, contributing to 65.9% of infertility in Tanzania. Lifestyle and oxidative stress (OS) have been suggested to affect female fertility; however, there are limited and controversial reports. This study aimed to determine the lifestyle and OS status of fertile and infertile women and relate lifestyle and OS to female infertility. The study was conducted among randomly selected 48 fertile and 48 infertile women aged 15–49 years attending a gynaecological clinic at Muhimbili National Hospital. Lifestyle information was collected using a proforma, and a sandwich ELISA method was used to analyse OS biomarkers (malondialdehyde and total antioxidant capacity). Chi-square, independent t test and correlation coefficient were used for data analysis at p < 0.05. Results Infertile women were significantly older (33.4 versus 30.3 years, p = 0.03), got married at later ages (25.0 versus 22.8 years, p = 0.02), were in employment (62.5% versus 29.2%, p = 0.001), had attained university degree (56.3% versus 27.1%, p = 0.004) and were more obese, more likely to consume alcoholic drinks (p = 0.02) and less likely to use supplements (p = 0.000) than fertile women. Infertile women had statistically significant higher malondialdehyde (p = 0.000) and lower total antioxidant capacity levels (p = 0.000) than fertile women. The total antioxidant capacity level had a statistically significant negative correlation with alcohol consumption (r = −0.27, p = 0.008) and obesity (r = −0.32, p = 0.002) and a positive correlation with supplement use (r = 0.24, p = 0.02). Also, malondialdehyde level had a statistically significant positive correlation with alcohol consumption (r = 0.31, p = 0.002) and obesity (r = 0.28, p = 0.007). Conclusions The differences in socio-demographic characteristics, lifestyle and oxidative stress between fertile and infertile women may account for difficulties in achieving pregnancy among infertile women in Tanzania. Lifestyle factors significantly affect oxidative stress. Health education on appropriate lifestyles that support female fertility and emphasis on supplement use during the preconception period may be beneficial in the management of female infertility.

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“…Vitamin E concentrations in serum samples were determined according to the protocol by Jargar et al [25]. Serum samples were slowly added to absolute ethanol with gentle shaking to denature the proteins.…”

Section: Measurements Of Fatty Acids Profile Vitamin E Selenium and L...mentioning

confidence: 99%

Consumption of Hen Eggs Enriched with n-3 Polyunsaturated Fatty Acids, Selenium, Vitamin E and Lutein Incites Anti-Inflammatory Conditions in Young, Healthy Participants — A Randomized Study

Šušnjara¹,

Kolobarić²,

Matić³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: Dietary supplementation with compounds that possess antioxidant and anti-inflammatory properties (n-3 polyunsaturated fatty acids (PUFAs), selenium, vitamin E, lutein), has been shown to positively correlate with improvements in chronic conditions, although understanding of these combined effects in healthy humans is limited. The study aimed to evaluate the effects of enriched eggs consumption on oxidative status and inflammatory conditions in healthy volunteers. We hypothesized that a three-week diet containing enriched eggs can alter the immune response of healthy adults towards anti-inflammatory conditions. Methods: 34 participants consumed 3 hard-boiled hen eggs per day (21 days): Control group-regular hen eggs (n-3 PUFAs = 438 mg, selenium = 0.054 mg, lutein = 0.330 mg and vitamin E = 1.785 mg) (N = 14); 4Nutri group-hen eggs enriched with 4 nutrients (n-3 PUFAs = 1026 mg, selenium = 0.06 mg, lutein = 1.85 mg and vitamin E = 3.29 mg) (N = 20). Samples were taken before and after the protocol. Serum concentrations of lipid mediators and cytokines were measured with enzyme-linked immunosorbent assay (ELISA) and antibody-based, magnetic bead reagent kits on the Luminex platform, respectively. Serum oxidative stress and antioxidant capacity were measured using standardized methods, while gene expression in peripheral blood mononuclear cells (PBMCs) was measured via real-time PCR. Results: Decreased serum levels of pro-inflammatory interleukin 17A (IL-17A) and an increased neuronal nitric oxide synthase (nNOS) expression in the 4Nutri group, together with alteration of metabolites produced via cyclooxygenase (COX) pathways in the Control group, suggest a shift towards anti-inflammatory conditions in participants who consumed enriched hen eggs. Conclusions: Present results suggest that the combined action of n-3 PUFAs and antioxidants may have a protective role in resting, non-inflammatory conditions. Clinical Trial Registration: NCT04564690.

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Status of and #945;-tocopherol concentration and oxidative stress in infertile females of Vijayapur District, northern Karnataka

Cited by 4 publications

References 20 publications

Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study

Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study

Lifestyle and oxidative stress status in infertile women in Dar es Salaam, Tanzania: comparative cross-sectional study

Consumption of Hen Eggs Enriched with n-3 Polyunsaturated Fatty Acids, Selenium, Vitamin E and Lutein Incites Anti-Inflammatory Conditions in Young, Healthy Participants — A Randomized Study

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