Status of alternaria leaf blight and leaf spot diseases of makhana in North Bihar and their management strategies under integrated approach

Kumar, Santosh; Akhtar, Md. Nadeem; Patel, Deepak Kumar; Saha, Tamoghna; Sah, S. B.

doi:10.1007/s42360-022-00497-6

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…The inoculation of P. maculans isolates on the young leaves of susceptible variety (Serpentina), revealed the consequential variation in incubation period and lesion development, indicating varied pathogenicity and virulence. Disease development on injured-and uninjured-leaves substantiated the pathogen entry through the natural openings (stomata) [5,14,24,25,26]. The infection was more on injured leaves inoculated on the undersurface containing more number of stomata that facilitated the entry of the pathogen [27,28].…”

Section: Discussionmentioning

confidence: 99%

“…Pathogenicity and pathogenic variability of the pathogen isolates was determined by detached leaf technique on healthy young leaves of susceptible variety (Serpentina) of mulberry [14]. Inoculations were carried out with mycelial bit of 5-millimetre (mm) diameter on upper and lower surfaces of leaves with and without injury.…”

Section: Pathogenicity Tests and Pathological Variabilitymentioning

confidence: 99%

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Morpho-cultural, pathological and molecular variability in Phloeospora maculans causing leaf spot of mulberry (Morus species) in India

Saif,

Ashraf,

Shah

et al. 2023

Mol Biol Rep

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Background:Leaf spot disease (LSD) of mulberry caused by Phloeospora maculans is a major threat to the silk industry of Jammu and Kashmir, India, therefore, it was necessary to study the population structure of the pathogen for successful management of the disease. Methods and ResultsTo understand the diversity in the Phloeospora maculans, a combination of conventional (morphological, cultural and pathological) and molecular (ISSR markers) approaches were employed to discern the variability in 27 isolates collected from Srinagar, Bandipora, and Baramulla districts of Jammu and Kashmir, India. The studies revealed a high level of variability in the pathogen. Based on the morphocultural and pathological studies, the pathogen isolates were grouped into different categories based on colony growth, texture, margin and colour besides changes in colour of medium, incubation period, leaf area infected, etc.A high level of polymorphism was observed in different isolates of P. maculans using ISSR markers, which indicated that these markers are suitable for studying the genetic diversity in this pathogen. All the isolates (27) of P. maculans were clustered into two groups or populations as indicated by mean delta K value. Analysis of molecular variance revealed the low genetic variation among the populations (1.08%) and a high level of genetic variation within the populations (98.91%). F st value was found to be 0.01 indicating smaller amount of genetic differentiation between the populations against calculated P-value of 0.29 ConclusionA high level of diversity based on morphological, cultural, pathological and molecular levels was observed in Phloeospora maculans collected from various districts of Kashmir valley, which indicates that the study of population structure is necessary for successful management of the disease.

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Section: Discussionmentioning

confidence: 99%

Section: Pathogenicity Tests and Pathological Variabilitymentioning

confidence: 99%

Morpho-cultural, pathological and molecular variability in Phloeospora maculans causing leaf spot of mulberry (Morus species) in India

Saif,

Ashraf,

Shah

et al. 2023

Mol Biol Rep

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Morpho-cultural, pathological and molecular variability in Phloeospora maculans causing leaf spot of mulberry (Morus species) in India

Saif,

Kirmani,

Shah

et al. 2023

Preprint

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Background: Leaf spot disease (LSD) of mulberry caused by Phloeospora maculans is a major threat to the silk industry of Jammu and Kashmir, India, therefore, it was necessary to study the population structure of the pathogen for successful management of the disease.Methods and Results To understand the diversity in the Phloeospora maculans, a combination of conventional (morphological, cultural and pathological) and molecular (ISSR markers) approaches were employed to discern the variability in 27 isolates collected from Srinagar, Bandipora, and Baramulla districts of Jammu and Kashmir, India. The studies revealed a high level of variability in the pathogen. Based on the morpho-cultural and pathological studies, the pathogen isolates were grouped into different categories based on colony growth, texture, margin and colour besides changes in colour of medium, incubation period, leaf area infected, etc.A high level of polymorphism was observed in different isolates of P. maculans using ISSR markers, which indicated that these markers are suitable for studying the genetic diversity in this pathogen. All the isolates (27) of P. maculans were clustered into two groups or populations as indicated by mean delta K value. Analysis of molecular variance revealed the low genetic variation among the populations (1.08%) and a high level of genetic variation within the populations (98.91%). Fst value was found to be 0.01 indicating smaller amount of genetic differentiation between the populations against calculated P-value of 0.29Conclusion A high level of diversity based on morphological, cultural, pathological and molecular levels was observed in Phloeospora maculans collected from various districts of Kashmir valley, which indicates that the study of population structure is necessary for successful management of the disease.

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Status of alternaria leaf blight and leaf spot diseases of makhana in North Bihar and their management strategies under integrated approach

Cited by 2 publications

References 6 publications

Morpho-cultural, pathological and molecular variability in Phloeospora maculans causing leaf spot of mulberry (Morus species) in India

Morpho-cultural, pathological and molecular variability in Phloeospora maculans causing leaf spot of mulberry (Morus species) in India

Morpho-cultural, pathological and molecular variability in Phloeospora maculans causing leaf spot of mulberry (Morus species) in India

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