Status in molluscan cell line development in last one decade (2010–2020): impediments and way forward

Balakrishnan, Soumya; Singh, I. S. Bright; Puthumana, Jayesh

doi:10.1007/s10616-022-00539-x

Cited by 9 publications

(18 citation statements)

References 152 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As seen from the many reported examples, several fish viruses are not cultivable in cell cultures; in fact, some of them can only hardly or very slowly replicate in vitro; furthermore, the use of virus isolation in cell cultures is barred from the study of shellfish diseases due to the lack of suitable cell cultures. As a matter of fact, despite decades of attempts, not even a single cell line of marine molluscs or crustaceans is available [ 165 , 166 ]. Cell cultures are systems with a sensitive balance that are often subjected to toxic samples.…”

Section: Discussionmentioning

confidence: 99%

Advances in Viral Aquatic Animal Disease Knowledge: The Molecular Methods’ Contribution

Volpe

Errani

Mandrioli

et al. 2023

Biology

View full text Add to dashboard Cite

Aquaculture is the fastest-growing food-producing sector, with a global production of 122.6 million tonnes in 2020. Nonetheless, aquatic animal production can be hampered by the occurrence of viral diseases. Furthermore, intensive farming conditions and an increasing number of reared fish species have boosted the number of aquatic animals’ pathogens that researchers have to deal with, requiring the quick development of new detection and study methods for novel unknown pathogens. In this respect, the molecular tools have significantly contributed to investigating thoroughly the structural constituents of fish viruses and providing efficient detection methods. For instance, next-generation sequencing has been crucial in reassignment to the correct taxonomic family, the sturgeon nucleo-cytoplasmic large DNA viruses, a group of viruses historically known, but mistakenly considered as iridoviruses. Further methods such as in situ hybridisation allowed objectifying the role played by the pathogen in the determinism of disease, as the cyprinid herpesvirus 2, ostreid herpesvirus 1 and betanodaviruses. Often, a combination of molecular techniques is crucial to understanding the viral role, especially when the virus is detected in a new aquatic animal species. With this paper, the authors would critically revise the scientific literature, dealing with the molecular techniques employed hitherto to study the most relevant finfish and shellfish viral pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Advances in Viral Aquatic Animal Disease Knowledge: The Molecular Methods’ Contribution

Volpe

Errani

Mandrioli

et al. 2023

Biology

View full text Add to dashboard Cite

show abstract

“…Most reports of successful attempts to culture molluscan cells indicated that primary cell cultures originating from various tissues, including hemolymph (hemocytes), heart, mantle, digestive gland and gill, can persist for extended periods in vitro, making them suitable systems for basic cell research (reviewed in [2]). Both short-and longer-term molluscan cell cultures have been exploited for advancing the understanding of complex biological phenomena and physiological systems [3]. One biological phenomenon specific to bivalve mollusks that could greatly benefit from cell culture experiments to be better understood is their unique mitochondrial transmission system.…”

Section: Introductionmentioning

confidence: 99%

“…are largely distributed, widely studied as environmental sentinels, extensively cultured in commercial mariculture (or mytiliculture) and they exhibit DUI. Cell cultures from different Mytilus species have been used in both applied and basic research [3]. For example, hemocytes and gill cells of Mytilus galloprovincialis have been used to assess cytotoxicity in several assays [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

In vitro proliferation of Mytilus edulis male germ cell progenitors

Hosseini Khorami,

Breton,

Angers

2024

PLoS ONE

View full text Add to dashboard Cite

Our understanding of basic cellular processes has mostly been provided by mammalian cell culture, and by some non-mammalian vertebrate and few invertebrate cell culture models. Developing reliable culture conditions for non-model organisms is essential to allow investigation of more unusual cellular processes. Here, we investigate how cells isolated from different tissues of the marine mussel Mytilus edulis thrive and survive in vitro in the hope of establishing a suitable laboratory model for the investigation of cellular mechanisms specific to these bivalve mollusks. We found that cells dissociated from mantle tissue attached to the culture vessels and proliferated well in vitro, whereas cells isolated from gills, although remaining viable, did not maintain divisions over three to four weeks in culture. We used antibodies against the germ-line marker DEAD-box helicase 4 (DDX4), also known as VASA, and the epithelial cell marker cytokeratin to distinguish different cell types in culture. DDX4-positive cells were predominant in 25-day-old cultures from male mantles. Cells from other tissues remained in low numbers and did not seem to change in composition over time. Overall, the culture conditions described here allow an efficient selection of male germ cells that could be used to study specific cellular mechanisms in vitro.

show abstract

“…Since the 1960s, over 500 peer-reviewed publications have been published on this topic, with the main focus being on six phyla, which are Porifera, Cnidaria, Crustacea, Mollusca, Echinodermata, and Urochordata [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. However, until recently [ 8 ], not a single continuous cell line was established for any aquatic invertebrate taxon, as all efforts to obtain lasting proliferating cultures from marine invertebrates have inexplicably failed [ 1 , 2 , 9 ], despite the increasing demand of these cultures for a wide range of applications [ 5 , 6 , 7 , 8 , 10 , 11 ]. Further, it has been repeatedly shown that primary cultures of marine invertebrate cells cease dividing 24–72 h from onset [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…It is worth noting that a substantial number of unsuccessful attempts to establish primary cell cultures from marine invertebrates are not reported in the scientific literature, resulting in incomplete or fragmented knowledge of effective in vitro protocols [ 7 , 12 ], and researchers often end up revisiting unsuccessful methods and futile experimental protocols [ 1 , 2 ]. Considering the above discussion, a recent advancement in sponge cell culture has emphasized the significance of developing optimized nutrient media for the development of primary cell cultures [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ], a research approach that has led to the succeeded establishment of long-lasting cultures from several sponge species [ 8 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Improved Media Formulations for Primary Cell Cultures Derived from a Colonial Urochordate

Qarri

Kültz

Gardell

et al. 2023

Cells

View full text Add to dashboard Cite

The cultivation of marine invertebrate cells in vitro has garnered significant attention due to the availability of diverse cell types and cellular potentialities in comparison to vertebrates and particularly in response to the demand for a multitude of applications. While cells in the colonial urochordate Botryllus schlosseri have a very high potential for omnipotent differentiation, no proliferating cell line has been established in Botryllus, with results indicating that cell divisions cease 24–72 h post initiation. This research assessed how various Botryllus blood cell types respond to in vitro conditions by utilizing five different refinements of cell culture media (TGM1–TGM5). During the initial week of culture, there was a noticeable medium-dependent increase in the proliferation and viability of distinct blood cell types. Within less than one month from initiation, we developed medium-specific primary cultures, a discovery that supports larger efforts to develop cell type-specific cultures. Specific cell types were easily distinguished and classified based on their natural fluorescence properties using confocal microscopy. These results are in agreement with recent advances in marine invertebrate cell cultures, demonstrating the significance of optimized nutrient media for cell culture development and for cell selection.

show abstract

Status in molluscan cell line development in last one decade (2010–2020): impediments and way forward

Cited by 9 publications

References 152 publications

Advances in Viral Aquatic Animal Disease Knowledge: The Molecular Methods’ Contribution

Advances in Viral Aquatic Animal Disease Knowledge: The Molecular Methods’ Contribution

In vitro proliferation of Mytilus edulis male germ cell progenitors

Improved Media Formulations for Primary Cell Cultures Derived from a Colonial Urochordate

Contact Info

Product

Resources

About