We describe the construction and calibration of a multi-channel liquid time projection chamber filled with Tetramethylsilane (TMS). Its charge readout system consists of 8 wires each in the X and Y directions. The chamber is also equipped with a Cherenkov light readout system consisting of a 5-inch photomultiplier tube (PMT) coupled to the liquid volume through a viewport. The energy scale of the detector is calibrated using positron-electron pairs produced by 4.4 MeV gamma rays emitted from an AmBe source, using an external trigger on the positron annihilation gammas. The external trigger is then reconfigured to tag cosmic ray muons passing through the active TMS volume, which are used to measure the stopping power in TMS and the electron lifetime in the detector. We find a most-probable energy loss from minimum ionising particles (MIPs) of ∆p/ds = (0.60±0.01) MeV/cm. We also derive an electron lifetime of 43 +680 −21 µs by measuring the mostprobable energy loss as a function of drift time. For both fast electron and muon signals, the PMT detects prompt Cherenkov light, demonstrating the possibility of random triggering. The roomtemperature organic target medium, together with the self-triggering capabilities and long electron lifetimes reported in this work, make this an attractive technology to further explore for rare event detectors or other applications in the area of radiation measurements.