Statistically significant correlation between normal sperm morphology (NSM) and sperm binding potential to an immobilized monolayer of hyaluronan using the HYDAK(r) sperm hyaluronan-binding assay (HBA)

Worrilow, K.C.; Belcak, N.; Maher, Nancy; Bower, J.B.; Peters, Albert J.; Johnston, J. H.

doi:10.1016/s0015-0282(03)01913-7

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“…Previous studies have shown that fertile human spermatozoa bind to glass slides coated with hyaluronic acid (Worrilow et al, 2003;Huszar et al, 2004;Roudebush et al, 2004). A binding assay (HBA; Medicult, Denmark) is commercially available whereby the binding of intact spermatozoa to glass slides coated with recombinant hyaluronic acid (hyaluronan) is thought to be indicative of the potential fertility of a human semen sample in subsequent IVF.…”

Section: Introductionmentioning

confidence: 99%

New Methods for Selecting Stallion Spermatozoa for Assisted Reproduction

Colleoni

Lagutina

Lazzari

et al. 2011

Journal of Equine Veterinary Science

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Improved sperm selection techniques are needed to increase the efficiency of equineassisted reproduction. Single layer centrifugation (SLC) of spermatozoa has been shown to improve the quality of stallion sperm samples. In this study, the functionality of selected stallion spermatozoa was tested by intracytoplasmic sperm injection of equine oocytes after selection by SLC through Androcoll-E or by discontinuous density gradient centrifugation (DGC) through Redigrad and Tyrode's medium with added albumin, lactate, and pyruvate. The mean cleavage rates of the injected oocytes from SLC-and DGC-selected spermatozoa were 67% and 66%, respectively, whereas the proportion of blastocysts developing from cleaved oocytes was 28% and 22%, respectively (P > .05, not significant). An incidental finding was that there was a tendency for SLC-selected spermatozoa to have a higher percentage of spermatozoa with normal morphology than DGC (70% ± 22% vs. 58% ± 38%) and for more blastocysts to be obtained from subfertile ejaculates (21 [19.6%] vs. 15 [14.4%], respectively). In further experiments, stallion spermatozoa bound to hyaluronan, although binding may depend on the semen extender and sperm treatment as well as incubation time. In conclusion, SLC-selected stallion spermatozoa function normally when injected into oocytes. SLC may potentially be better than DGC at selecting spermatozoa from subfertile ejaculates, but this effect needs rigorous investigation with a much larger sample size. Use of the hyaluronanbinding assay for assessing the potential fertility of stallion spermatozoa may be useful but requires further evaluation.

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Section: Introductionmentioning

confidence: 99%