Khanal, R., Navabi, A. and Lukens, L. 2015. Linkage map construction and quantitative trait loci (QTL) mapping using intermated vs. selfed recombinant inbred maize line (Zea mays L.). Can. J. Plant Sci. 95: 1133Á1144. Intermating of individuals in an F 2 population increases genetic recombination between markers, which is useful for linkage map construction and quantitative trait loci (QTL) mapping. The objectives of this study were to compare the linkage maps and precision of QTL detection in an intermated recombinant inbred line (IRIL) population and a selfed recombinant inbred line (RIL) population. Both, IRIL and RIL, populations were developed from Zea mays inbred lines CG60 and CG102. The populations were grown in two environments to evaluate traits, and inbred lines from each population were genotyped with SSR and SNP markers for linkage map construction and QTL identification. In addition, we simulated RIL and IRIL populations from two inbred parents to compare the precision of QTL detection between simulated RIL and IRIL populations. In the empirical study, the linkage map was longer in RIL as compared with IRIL, and the average QTL support interval was reduced by 1.37-fold in the IRIL population compared with the RIL population. We detected 16 QTL for flowering time, plant height, leaf number, and stay green in at least one recombinant inbred line population. Two out of 16 QTL were shared between two recombinant inbred line populations. In the simulation study, the QTL support interval was reduced by 1.66-fold in the IRIL population as compared with the RIL population and linked QTL were identified more frequently in IRIL population as compared with RIL population. This study supports the utility of intermated RIL populations for precise QTL mapping. Croiser les individus de la F 2 accroıˆt la recombinaison des marqueurs, ce qui facilite la construction de cartes ge´ne´tiques et la situation des locus quantitatifs (QTL) sur ces cartes. La pre´sente e´tude devait comparer les cartes ge´ne´tiques e´tablies avec une population de ligne´es recombinantes hybrides (IRIL) ou pures (RIL) et la pre´cision avec laquelle ces populations permettent de de´tecter les QTL. Les populations IRIL et RIL ont e´te´obtenues a`partir des ligne´es autogames de Zea mays CG60 et CG102. Elles ont e´te´cultive´es dans deux environnements, ce qui a permis d'en e´valuer les caracte`res, et on a e´tabli le ge´notype des ligne´es autogames de chaque population avec des marqueurs SSR et SNP pour baˆtir une carte ge´ne´tique et identifier les QTL. Les auteurs ont aussi simule´les populations RIL et IRIL a`partir de deux parents autogames en vue de comparer la pre´cision avec laquelle ces populations permettent de de´tecter les QTL. Lors de l'e´tude empirique, la carte ge´ne´tique de la population RIL e´tait plus grande que celle de la population IRIL et l'intervalle de support moyen des QTL e´tait plus court de 1,37 fois chez la population IRIL. Les auteurs ont de´tecte´16 QTL correspondant au moment de la floraison, a`la hauteur du p...