Statistical power for detecting epistasis QTL effects under the F-2 design

Mao, Yongcai; Da, Yang

doi:10.1051/gse:2004041

Cited by 2 publications

(2 citation statements)

References 11 publications

(25 reference statements)

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“…Although an F 2 intercross with 310 animals is underpowered to identify epistatic loci (Mao and Da 2005), the DobzhanskyMuller model suggests that the hybrid male sterility that we observed is indeed caused by multi-locus incompatibilities.…”

Section: Dobzhansky-muller Incompatibilities With the X Chromosomementioning

confidence: 97%

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

et al. 2011

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Reproductive isolation between species is often caused by deleterious interactions among loci in hybrids. Finding the genes involved in these incompatibilities provides insight into the mechanisms of speciation. With recently diverged subspecies, house mice provide a powerful system for understanding the genetics of reproductive isolation early in the speciation process. Although previous studies have yielded important clues about the genetics of hybrid male sterility in house mice, they have been restricted to F 1 sterility or incompatibilities involving the X chromosome. To provide a more complete characterization of this key reproductive barrier, we conducted an F 2 intercross between wild-derived inbred strains from two subspecies of house mice, Mus musculus musculus and Mus musculus domesticus. We identified a suite of autosomal and X-linked QTL that underlie measures of hybrid male sterility, including testis weight, sperm density, and sperm morphology. In many cases, the autosomal loci were unique to a specific sterility trait and exhibited an effect only when homozygous, underscoring the importance of examining reproductive barriers beyond the F 1 generation. We also found novel two-locus incompatibilities between the M. m. musculus X chromosome and M. m. domesticus autosomal alleles. Our results reveal a complex genetic architecture for hybrid male sterility and suggest a prominent role for reproductive barriers in advanced generations in maintaining subspecies integrity in house mice.

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Section: Dobzhansky-muller Incompatibilities With the X Chromosomementioning

confidence: 97%

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

et al. 2011

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“…This apparent contradiction between our results and those of previous studies is likely to be the result of our low power to detect epistasis. Detecting epistasis is far more difficult than detecting single QTL and requires relatively big samples sizes (n > 400), especially in the case of interactions involving dominance effects (Mao and Da 2005; Wei et al 2010). Dominance is an important component of variance in beak length in soapberry bugs (Carroll et al 2001; Carroll 2007).…”

Section: Resultsmentioning

confidence: 99%

Genetic Architecture of Contemporary Adaptation to Biotic Invasions: Quantitative Trait Locus Mapping of Beak Reduction in Soapberry Bugs

Andrés

2014

G3 Genes|Genomes|Genetics

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Biological invasions can result in new selection pressures driven by the establishment of new biotic interactions. The response of exotic and native species to selection depends critically on the genetic architecture of ecologically relevant traits. In the Florida peninsula, the soapberry bug (Jadera haematoloma) has colonized the recently introduced Chinese flametree, Koelreuteria elegans, as a host plant. Driven by feeding efficiency, the populations associated with this new host have differentiated into a new bug ecomorph characterized by short beaks more appropriate for feeding on the flattened pods of the Chinese flametree. In this study, we have generated a three-generation pedigree from crossing the long-beaked and short-beaked ecomorphs to construct a de novo linkage map and to locate putative quantitative trait locus (QTL) controlling beak length and body size in J. haematoloma. Using amplified fragment-length polymorphism markers and a two-way pseudo-testcross design, we have produced two parental maps in six linkage groups, covering the known number of chromosomes. QTL analysis revealed one significant QTL for beak length on a maternal linkage group and the corresponding paternal linkage group. Three QTL were found for body size. Through single marker regression analysis, nine single markers that could not be placed on the map were also found to be significantly associated with one or both of the two traits. Interestingly, the most significant body size QTL co-localized with the beak length QTL, suggesting linkage disequilibrium or pleiotropic effects of related traits. Our results suggest an oligogenic control of beak length.

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Statistical power for detecting epistasis QTL effects under the F-2 design

Cited by 2 publications

References 11 publications

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

Genetic Architecture of Contemporary Adaptation to Biotic Invasions: Quantitative Trait Locus Mapping of Beak Reduction in Soapberry Bugs

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